A proteomics research of apoptosis of leukemia cells induced by arsenic trioxide

L. Wang; Q. Guo; W. Liu; Y. Bai

doi:10.14715/cmb/2017.63.3.2

Issue

Vol. 63 No. 3: Issue 3

Issue Published : May 3, 2017

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A proteomics research of apoptosis of leukemia cells induced by arsenic trioxide

https://doi.org/10.14715/cmb/2017.63.3.2

L. Wang

Chengdu Women's and Children's Central Hospital, Chengdu, Sichuan Province, China

Q. Guo

Department of Paediatrics, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China

W. Liu

Department of Paediatrics, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China

Y. Bai

Department of Paediatrics, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China

Corresponding Author(s) : W. Liu

wenjunliu1@126.com

Cellular and Molecular Biology, Vol. 63 No. 3: Issue 3
Article Published : March 31, 2017

Abstract

Leukemia is a malignant hyperplasia disease of hematopoietic system caused by a kind of hematopoietic cells detaining at a certain stage. In this study, we used HL-60 cell line to detect the effect of apoptosis induction by various concentrations of As2O3. Percentage of apoptotic cells was increased in the presence of As2O3, detected through flow cytometry. Mass spectrometric identification indicated that there were 102 differential proteins before and after intervention. Among them, expression of CH60, AT1A1, ANXA6, and EF2 increased and expression of ATPA, MYO1G, RS3 decreased. Those genes played roles in promoting HL-60 cell apoptosis induced by As2O3. This study could lay the foundation of deciphering mechanisms of HL-60 cell apoptosis and give a new possible target to the clinical application for arsenic trioxide treatment for leukemia.

Keywords

HL-60 cells Arsenic trioxide Apoptosis Differences protein.

Wang, L., Guo, Q., Liu, W., & Bai, Y. (2017). A proteomics research of apoptosis of leukemia cells induced by arsenic trioxide. Cellular and Molecular Biology, 63(3), 7–11. https://doi.org/10.14715/cmb/2017.63.3.2