Effects of life cycle and leaves location on gene expression and glycoside biosynthesis pathway in Stevia rebaudiana Bertoni

Matin Ghaheri; Elaheh Adibrad; Seyed Mehdi Safavi; Danial Kahrizi; Ali Soroush; Saare Muhammadi; Tayebeh Ghorbani; Ali Sabzevari; Zahra Ansarypour; Elham Rahmanian

doi:10.14715/cmb/2018.64.2.4

Issue

Vol. 64 No. 2: New biotechnological aspects in Stevia rebaudiana Bertoni

Issue Published : February 11, 2018

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Effects of life cycle and leaves location on gene expression and glycoside biosynthesis pathway in Stevia rebaudiana Bertoni

https://doi.org/10.14715/cmb/2018.64.2.4

Matin Ghaheri

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Elaheh Adibrad

Department of Medicinal Plants, Institute of Higher Education, Jahad-e-Daneshgahi, Kermanshah Unit, Iran

Seyed Mehdi Safavi

Department of Agronomy and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran

Danial Kahrizi

Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Ali Soroush

Department of Rehabilitative and sports medicine. Kermanshah University of Medical Sciences, Kermanshah, Iran

Saare Muhammadi

Department of Cardiology, Kermanshah University of Medical Sciences, Kermanshah, Iran

Tayebeh Ghorbani

Zagros Bioidea Company, Kermanshah, Iran

Ali Sabzevari

Faculty of Paramedical Sciences, Shahrekord University of Medical Sciences, Shahrekord, Iran

Zahra Ansarypour

Isfahan University of Technology, College of Agriculture, Department of Biotechnology, Isfahan, Iran

Elham Rahmanian

The Rhazes Center for Research in Family Health and Sexual Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Corresponding Author(s) : Danial Kahrizi

dkahrizi@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 2: New biotechnological aspects in Stevia rebaudiana Bertoni
Article Published : February 10, 2018

Abstract

Stevia rebaudiana Bertoni is One of the most important biologically sourced and low-calorie sweeteners that known as "Sweet Weed”. It contains steviol glycosides that they are about 200-300 times sweeter than sucrose. Tissue culture is the best method with high efficiency that can overcome to problems of traditional methods, and it is the most useful tools for studying stress tolerance mechanisms under in vitro conditions to obtain drought tolerance. In the present research, we investigated the impact of life cycle, leaves location and the harvesting time on expression of UGT74G1 and UGT76G1 as well as steviol glycosides accumulation. The highest gene expression of both UGT74G1 and UGT76G1 (207.677 and 208.396 Total Lab unit, respectively) was observed in young leaves in the second vegetative year. Also, the highest amount of stevioside accumulation (13.04) was due to the old leaves in vegetative stage which had significant differences with other effects whereas the lowest accumulation (7.47) was seen at young leaves at vegetative stage. Interestingly, the highest level of rebaudioside a production (15.74) was occurred at the young leaves at vegetative stage. There was significant differences between life cycle and leaves location on steviol glycoside production in stevia.

Keywords

Stevia rebaudiana Bertoni Semi-quantitative RT- PCR HPLC Life cycle Leaves location UGT76G1 UGT74G1.

Ghaheri, M., Adibrad, E., Safavi, S. M., Kahrizi, D., Soroush, A., Muhammadi, S., Ghorbani, T., Sabzevari, A., Ansarypour, Z., & Rahmanian, E. (2018). Effects of life cycle and leaves location on gene expression and glycoside biosynthesis pathway in Stevia rebaudiana Bertoni. Cellular and Molecular Biology, 64(2), 17–22. https://doi.org/10.14715/cmb/2018.64.2.4

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References

Anbazhagan M, Kalpana M, Rajendran R, Natarajan V, Dhanavel D. In vitro production of Stevia rebaudiana Bertoni. Emir J food agric 2010; 22:216-222.
Singh S, Garg V, Yadav D, Beg MN, Sharma N. In-vitro antioxidative and antibacterial activities of various parts of stevia rebaudiana (Bertoni). Int J Pharm Pharm Sci 2012; 4(3):468-473.
Prakash I, Markosyan A, Bunders C. Development of Next Generation Stevia Sweetener: Rebaudioside M. Foods 2014; (3):162-175
Mondaca RL, Glvez AV, Bravo LZ, Hen KA. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem 2012; 132:1121-1132.
Chalapathi MV, Thimmegowda S. Natural non-calorie sweetener stevia (Stevia rebaudiana Bertoni): A future crop of India. Crop Res Hisar 1997; 14 (2):347-350
Liu J, Li SFY. Separation and determination of stevia sweeteners by capillary electrophoresis and high performance liquid chromatography. J Liq Chromatogr 1995; 18 (9):1703-1719
Jaroslav P, Barbora H, Tuulia H. Characterization of Stevia rebaudiana by comprehensive two-dimensional liquid chromatography time-of-flight mass spectrometry. J Pharm Pharm Sci 2006; 4(3):468-473.
Yadav P, Kumari P, Arya A, Tripathi S, Kumar S. Effect of nitrogen sources on rooting of in vitro culture of Stevia rebaudiana Bertoni. J Biotechnol. 2013; 4:41-46.
Verma R, Gena DD, Lal Jat B. In vitro propagation of Stevia rebaudiana Bertoni (a sweeting plant). J Pharm Res 2016; 5666-685.
Soufi S, D'Urso G, Pizza C, Rezgui S, Bettaieb T, Montoro. Steviol glycosides targeted analysis in leaves of Stevia rebaudiana (Bertoni) from plants cultivated under chilling stress conditions. J Food Chem 2016; 190:572–580.
Debnath M. Propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. J Med Plant Res 2008;2: 45–51.
AL-Taha HAK. Effect of shock and gradual drought by PEG on callus growth and proline accumulation in sour orange (Citrus aurantium). Adv Agric Bot Int J Bio Soc 2013; 5(2):77-83.
Abdul Razak UNA, Ong CB, Yu TS, Lau LK. In vitro Micropropagation of Stevia rebaudiana Bertoni in Malaysia. Braz Arch Biol Technol 2014; 1:23-28.
Raina R, Bhandari SK, Chand R, Sharma Y. Strategies to improve poor seed germination in Stevia rebaudiana, a low calorie sweetener. J Med Plants Res 2013; 7:1793-1799.
Savita S, Sheela K, Sunanda S, Shankar A, Ramakrishna P. Stevia rebaudiana–a functional component for food industry. J Hum Ecol 2004; 15:261–264.
Kamran Khan M, Misra P, Sharma P, Shukla PK, Ramteke PW. Effect of adenine sulphate on in vitro mass propagation of Stevia rebaudiana Bertoni. J Med Plants Res 2014; 8:543-549.
Mukundan U, Sivaram L. In vitro culture studies on Stevia rebaudiana. J In Vitro Cell Dev Biol 2003; 5:520-523.
Kinghorn AD, Soejarto DD. Current status of stevioside as a sweetening agent for human use. In: Wagner, H., Hikino, H., Farnsworth, N.R. (Eds.), In J Eco Med Plant Res 1985; (1):1–52.
Tavarini S, Angelini LG. Stevia rebaudiana Bertoni as a source of bioactive compounds: the effect of harvest time, experimental site and crop age on steviol glycoside content and antioxidant properties. J Sci Food Agr 2013; 93(9):2121-9.
Kumar H, Kaul K, Bajpai-Gupta S, Kumar Kaul V, Kumar S. A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway in Stevia rebaudiana (Bertoni). Gene. 2011; 9(4):37-55.
Richman A, Swanson A, Humphrey T, Chapman R, McGarvey B, Pocs R, et al. Functional genomics uncovers three glucosyltransferases involved in the synthesis of the major sweet glucosides of Stevia rebaudiana. Plant J 2005; 41:56-67.
Brandle J, Starratt A, Gijzen M. Stevia rebaudiana: its agricultural, biological, and chemical properties. Canadian J Plant Sci 1998; 78(4):527-36.
Marone M, Mozzetti S, Ritis DD, Pierelli L, Scambia G. Semi quantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol Proced Online 2001; 3(1):19-25.
Jaitak V, Singh BB, Kaul VK. An efficient microwave-assisted extraction process of stevioside and rebaudioside-A from Stevia rebaudiana (Bertoni). Phytochem Anal 2009; 20:240–245.

References

Anbazhagan M, Kalpana M, Rajendran R, Natarajan V, Dhanavel D. In vitro production of Stevia rebaudiana Bertoni. Emir J food agric 2010; 22:216-222.

Singh S, Garg V, Yadav D, Beg MN, Sharma N. In-vitro antioxidative and antibacterial activities of various parts of stevia rebaudiana (Bertoni). Int J Pharm Pharm Sci 2012; 4(3):468-473.

Prakash I, Markosyan A, Bunders C. Development of Next Generation Stevia Sweetener: Rebaudioside M. Foods 2014; (3):162-175

Mondaca RL, Glvez AV, Bravo LZ, Hen KA. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem 2012; 132:1121-1132.

Chalapathi MV, Thimmegowda S. Natural non-calorie sweetener stevia (Stevia rebaudiana Bertoni): A future crop of India. Crop Res Hisar 1997; 14 (2):347-350

Liu J, Li SFY. Separation and determination of stevia sweeteners by capillary electrophoresis and high performance liquid chromatography. J Liq Chromatogr 1995; 18 (9):1703-1719

Jaroslav P, Barbora H, Tuulia H. Characterization of Stevia rebaudiana by comprehensive two-dimensional liquid chromatography time-of-flight mass spectrometry. J Pharm Pharm Sci 2006; 4(3):468-473.

Yadav P, Kumari P, Arya A, Tripathi S, Kumar S. Effect of nitrogen sources on rooting of in vitro culture of Stevia rebaudiana Bertoni. J Biotechnol. 2013; 4:41-46.

Verma R, Gena DD, Lal Jat B. In vitro propagation of Stevia rebaudiana Bertoni (a sweeting plant). J Pharm Res 2016; 5666-685.

Soufi S, D'Urso G, Pizza C, Rezgui S, Bettaieb T, Montoro. Steviol glycosides targeted analysis in leaves of Stevia rebaudiana (Bertoni) from plants cultivated under chilling stress conditions. J Food Chem 2016; 190:572–580.

Debnath M. Propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. J Med Plant Res 2008;2: 45–51.

AL-Taha HAK. Effect of shock and gradual drought by PEG on callus growth and proline accumulation in sour orange (Citrus aurantium). Adv Agric Bot Int J Bio Soc 2013; 5(2):77-83.

Abdul Razak UNA, Ong CB, Yu TS, Lau LK. In vitro Micropropagation of Stevia rebaudiana Bertoni in Malaysia. Braz Arch Biol Technol 2014; 1:23-28.

Raina R, Bhandari SK, Chand R, Sharma Y. Strategies to improve poor seed germination in Stevia rebaudiana, a low calorie sweetener. J Med Plants Res 2013; 7:1793-1799.

Savita S, Sheela K, Sunanda S, Shankar A, Ramakrishna P. Stevia rebaudiana–a functional component for food industry. J Hum Ecol 2004; 15:261–264.

Kamran Khan M, Misra P, Sharma P, Shukla PK, Ramteke PW. Effect of adenine sulphate on in vitro mass propagation of Stevia rebaudiana Bertoni. J Med Plants Res 2014; 8:543-549.

Mukundan U, Sivaram L. In vitro culture studies on Stevia rebaudiana. J In Vitro Cell Dev Biol 2003; 5:520-523.

Kinghorn AD, Soejarto DD. Current status of stevioside as a sweetening agent for human use. In: Wagner, H., Hikino, H., Farnsworth, N.R. (Eds.), In J Eco Med Plant Res 1985; (1):1–52.

Tavarini S, Angelini LG. Stevia rebaudiana Bertoni as a source of bioactive compounds: the effect of harvest time, experimental site and crop age on steviol glycoside content and antioxidant properties. J Sci Food Agr 2013; 93(9):2121-9.

Kumar H, Kaul K, Bajpai-Gupta S, Kumar Kaul V, Kumar S. A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway in Stevia rebaudiana (Bertoni). Gene. 2011; 9(4):37-55.

Richman A, Swanson A, Humphrey T, Chapman R, McGarvey B, Pocs R, et al. Functional genomics uncovers three glucosyltransferases involved in the synthesis of the major sweet glucosides of Stevia rebaudiana. Plant J 2005; 41:56-67.

Brandle J, Starratt A, Gijzen M. Stevia rebaudiana: its agricultural, biological, and chemical properties. Canadian J Plant Sci 1998; 78(4):527-36.

Marone M, Mozzetti S, Ritis DD, Pierelli L, Scambia G. Semi quantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol Proced Online 2001; 3(1):19-25.

Jaitak V, Singh BB, Kaul VK. An efficient microwave-assisted extraction process of stevioside and rebaudioside-A from Stevia rebaudiana (Bertoni). Phytochem Anal 2009; 20:240–245.