Issue

Vol. 64 No. 9: Issue 9

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Influence of abiotic elicitors on improvement production of artemisinin in cell culture of Artemisia annua L.

https://doi.org/10.14715/cmb/2018.64.9.1
Alireza Zebarjadi
Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran
Saeideh Dianatkhah
Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran
Payam Pour Mohammadi
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ramin University of Agriculture and Natural Resources, Ahwaz, Iran
Ardeshir Qaderi
Medicinal Plant Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

Corresponding Author(s) : Alireza Zebarjadi

zebarjadiali@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 9: Issue 9

Abstract

A significant sesquiterpene lactone used as a drug is artemisinin. It is definitely an anti-parasitic drug isolated from field-grown Artemisia annua L. a plant from Asteraceae family. It is the best treatment for Plasmodium falciparum malaria. Unfortunately, artemisinin content in A. annua is extremely low (0.01-0.8% dry weight). So, some researchers focused on enhancing artemisinin content either in tissue/cell culture or the whole plant of A. annua sp. The aims of the current study were the effect of plant growth regulators on callus production and improvement of artemisinin content in cell suspension culture of A. annua, an alternative to the whole plant using abiotic elicitors. For callus induction, an experiment was laid out as a factorial experiment with three factors (explant type, different concentrations of BAP and 2,4-D) based on completely randomized design with three replications. The maximum frequency of callus induction (100%) was found in leaf explant on MS medium with a combination of 2, 4-D (3 mg/l) and BAP (1.5 mg/l). Therefore, the best calli were used for cell suspension culture and the effects of GA3 and ABA as abiotic elicitors were evaluated on the improvement of artemisinin production. The results indicated that both ABA and GA3 increased artemisinin content (2.02 fold and 1.67 fold in comparison to control respectively) in cell suspension culture.

Keywords

Artemisinin Cell suspension culture Plant growth regulators Artemisia annua.
Zebarjadi, A., Dianatkhah, S., Pour Mohammadi, P., & Qaderi, A. (2018). Influence of abiotic elicitors on improvement production of artemisinin in cell culture of Artemisia annua L. Cellular and Molecular Biology, 64(9), 1–5. https://doi.org/10.14715/cmb/2018.64.9.1
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Tripathi L, Tripathi JN. Role of biotechnology in medicinal plants. Trop J Pharm Res 2003; 2(2): 243-253.
  2. Grech-Baran M, Pietrosiuk A. Artemisia species in vitro cultures for production of biologically active secondary metabolites. Bio Technologia 2012; 93(4): 371-380.
  3. Liu CZ, Murch SJ, El-Demerdash M, Saxena PK. Artemisia judaica L. micropropagation and antioxidant activity. J Biotechnol 2004; 110: 63-71.
  4. Stojanowska A. In vitro cultures of some plants from the Asteraceae family as a source of bioactive molecules. International Conference of Perspectives Cytostatic Compounds Production Using Biotechnological Methods, Warsaw 13 April 2010; 2010: 152.
  5. Kazemi M, Dakhili M, Dadkhah A, Yasrebifar Z, Larijani K. Composition, antimicrobial and antioxidant activities of the essential oil of Artemisia kermanensis Podl., an endemic species from Iran. J Med Plants Res 2011; 5(18): 4481-4486.
  6. Nakase I, Lai H, Singh NP, Sasaki T. Anticancer properties of artemisinin derivatives and their targeted delivery by transferrin conjugation. Int J Pharm 2008; 342(1-2): 28-33.
  7. Klayman DL. Qinghaosu (Artemisinin) an anti-malarial drug from China. Science 1985; 228: 1049-1055.
  8. Liu CZ, Zhao Y, Wang YC. Artemisinin: current state and perspectives for biotechnological production of an antimalarial drug. Appl Microbiol Biotechnol 2006; 72: 11-20.
  9. Weathers PJ, Elkholy S, Wobbe KK. Artemisinin: the biosynthetic pathway and its regulation in Artemisia annua, a terpenoid-rich species. In Vitro Cell Dev BiolPlant 2006; 42: 309-317.
  10. Hommel M. The future of artemisinins: natural, synthetic or recombinant. J Biol Sci 2008; 7: 38-42.
  11. WHO. World Malaria Report. http://www.who.int/malaria/publications/world-malaria-report-2016/report/en/ ed: http://www.who.int/malaria/publications/world-malaria-report-2016/report/en/; 2016.
  12. Abdin MZ, Israr M, Rehman RU, Jain SK. Artemisinin, a novel antimalarial drug: biochemical and molecular approaches for enhanced production. Planta Medica 2003; 69: 289-229.
  13. Xu XX, Zhu J, Huang DZ, Zhou WS. Total synthesis of artemisinin and deoxyartemisinin. Tetrahedron 1986; 42: 819-828.
  14. Zhao Y, Hantan WK, Lee KH. Antimalarial agents, artesunate, an inhibitor of cytochrome oxidase activity in Plasmodium berghei. J Nat Prod 1986; 49: 139-142.
  15. Van Geldre E, Vergauwe A, Van den E. State of the art of the production of the antimalarial compound artemisinin in plants. Plant Mol Biol 1997; 33: 199–209
  16. Ramachandra Rao S, Ravishankar GA. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol Adv 2002; 20(2): 101-153.
  17. Soomro R, Memon RA. Establishment of callus and suspension culture in Jatropha curcas. Pakistan Journal of Botany 2007; 39(7): 2431-2441.
  18. Hippolyte I, Marin B, Baccou J, Jonard R. Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L. Plant Cell Rep 1992; 11(3): 109-112.
  19. Baldi A, Dixit VK. Yield enhancement strategies for artemisinin production by suspension cultures of Artemisia annua. Cur Trends Biotechnol Pharm 2008; 99: 4609-4614.
  20. Murashige T, Skoog FK. Revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 1962; 15(3): 473-497.
  21. Dangash A, Ram M, Niranjan R, Bharillya A, Misra H. In vitro Selection and Hormonal Regulation in Cell Culture of Artemisia annua L. Plant. 2015; 3(1).
  22. Aslam N, Zia M, Chaudchary MF. Callogenesis and direct organogenesis of Artemisia scoparia. Pak J Biol Sci 2006; 9: 1783-1786..
  23. Ganesan CM, Paulsamy S. Standardized protocol for the in vitro culture of Artemisia annua L. – A medicinal plant at high altitudes of Nilgiris, the Western Ghats. J Res Biol 2011; 1(3): 173-178.
  24. Zare- Mehrjerdi M, Bihamta M, Omidi M, Naghavi M, Soltanloo H. Effects of exogenous methyl jasmonate and 2 isopentenyladenine on artemisinin production and gene expression in Artemisia annua. Tubitak 2013; 37: 499-505.
  25. Jing F, Zhang L, Li M et al. Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway. Biologia 2009; 64(2): 319-323.
  26. Smet ID, Zhang H, Inze D, Beeckman T. A novel role for abscisic acid emerges from underground. Trends Plant Sci 2006; 11: 434-439.
  27. Smith JI, Smart NJ, Kurz WG, Misawa M. Stimulation of indole alkaloid production in cell suspension cultures of Catharanthus roseus by abscisic acid. Planta Med 1987; 53: 470-474.
  28. Jing F, Zhang L, Li M et al. Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway. Biologia 2009; 64(2): 319-323.
  29. Fulzele DP, Heble MR, Rao PS. Production of terpenoid from Artemisia annua L. plantlet cultures in bioreactor. J Biotechnol 1995; 40: 139-143.
  30. Weathers PJ, Bunk G, Mccoy MC. The effect of phytohormones on growth and artemisinin production in Artemisia annua hairy roots. In Vitro CellDev Biol Plant 2005; 41: 47-53.
  31. Zhang YS, Ye HC, Liu BY, Wang H, Li GF, Russian J. Exogenous GA and flowering induce the conversion of artemisinic acid to artemisinin in Artemisia annua plants. Plant Physiol 2005; 52: 58-62.
  32. Woerdenbag HJ, Pras N, Chan NG et al. Artemisinin, related sesquiterpenes, and essential oil in Artemisia annua during a vegetation period in Vietnam. Planta Med 1994; 60: 272-275.
  33. Smith TC, Weathers PJ, Cheetham RC. Effect of gibberellic acid on hair root cultures of Artemisia annua growth and artemisinin production. In Vitro Cell Dev Biol Plant 1997; 33: 75-73.
  34. Crozier A, Kamiya Y, Bishop G, Yokota T. Biosynthesis of Hormones and Elicitor Molecules. In: Biochemistry and Molecular Biology of Plants. Buchanan, B., Guissem, W., and R. Jones.eds. . American Society of Plant Physiologist, Rockville, MD 2000: 850-929.
  35. Bunk GJ. Hormone influences on growth and secondary metabolite production in A. annua. M.S. Thesis. Worcester Polytechnic Institute, Worcester, MA USA 1997.
  36. Morales MR, Charles DJ, Simon JE. Seasonal accumulation of artemisinin in Artemisia annua L. Acta Hortic 1993; 344: 416-420.
  37. Ferreira JFS, Simon JE, Janick J. Developmental studies of Artemisia annua: Flowering and artemisinin production under greenhouse and field conditions. Planta Med 1995; 61: 167-170.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 1229 Download : 479