The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
In vitro antiproliferative effect and induction of apoptosis by Retama monosperma L. extract in human cervical cancer cells
Corresponding Author(s) : M. El mzibri
elmzibri@cnesten.org.ma
Cellular and Molecular Biology,
Vol. 57 No. 2: General Papers
Abstract
The antiproliferative effect of different extracts obtained from Retama monosperma L. was investigated on human SiHa and HeLa cervical cancer cell lines using a MTT colorimetric assay. The Retama monosperma L. dichloromethane fraction (Rm-DF) was the most active extract, exhibiting a significant cytotoxic activity on both cell lines in a dose-dependent manner, after 72h of treatment. IC50 values obtained were 14.57±4.15µg/ml and 21.33±7.88µg/ml, for SiHa and HeLa cell lines respectively. The morphological features assessment of apoptosis in Rm-DF-treated cells showed a condensation of chromatin and apoptotic bodies, accompanied by a decrease in mitochondrial membrane potential (ΔΨm) and an increase in reactive oxygen species in both cell lines. The induction of apoptosis was further confirmed by Western blotting pro-caspase 3, Bcl2 and PARP; caspase 3 activity assay; and Annexin V labelling. Analysis of Rm-DF by CG/MS revealed the presence of five known quinolizidine alkaloids as well as, sparteine (10,97%), L- methyl cytisine (9.11%), 17- oxosparteine (3.49%), lupanine (0.93%) and anagyrine (39.63%). This study shows that Retama monosperma L. extract exhibits a potential anticancer activity against cervical cancer cell lines in vitro through the inhibition of proliferation and induction of apoptosis, which may involve a mitochondria-mediated signaling pathway.
Keywords
Retama monosperma L.
in vitro cytotoxicity
apoptosis
cervical cancer cell lines
quinolizidine alkaloids.
Merghoub, N., Benbacer, L., El btaouri, H., Ait benhassou, H., Terryn, C., Attaleb, M., Madoulet, C., Benjouad, A., El mzibri, M., Morjani, H., & Amzazi, S. (2011). In vitro antiproliferative effect and induction of apoptosis by Retama monosperma L. extract in human cervical cancer cells. Cellular and Molecular Biology, 57(2), 1581–91. Retrieved from http://mail.cellmolbiol.org/index.php/CMB/article/view/944
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX