The influence of dimethyl sulfoxide (DMSO) on metabolic activity and morphology of melanoma cell line WM-266-4

Polonca Ferk; Barbara Dariš

doi:10.14715/cmb/2018.64.11.8

Issue

Vol. 64 No. 11: Issue 11

Issue Published : August 31, 2018

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The influence of dimethyl sulfoxide (DMSO) on metabolic activity and morphology of melanoma cell line WM-266-4

https://doi.org/10.14715/cmb/2018.64.11.8

Polonca Ferk

Institute for Biostatistics and Medical Informatics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia-EU

Barbara Dariš

Department of Cell Biology, Faculty of Medicine, University of Maribor, Taborska 8, SI-2000 Maribor, Slovenia-EU

Corresponding Author(s) : Polonca Ferk

barbara.daris@um.si

Cellular and Molecular Biology, Vol. 64 No. 11: Issue 11
Article Published : August 30, 2018

Abstract

Dimethyl sulphoxide (DMSO) is widely used as a solvent in biomedical research, regularly in concentrations up to 1%. Nevertheless, little is known about the effect of different DMSO concentration on WM-266-4 metastatic melanoma cells, which are often used in melanoma research. Due to resistance of melanoma cells high concentrations of cytotoxic substances soluble in DMSO are used in vitro tests. Consequently, total DMSO concentration often exceeds 1%. The aim of our study was to test the metabolic activity and morphology of WM-266-4 cells exposed to selected DMSO concentrations for different period of time. Cells were incubated in selected ethanol concentrations for comparison. MTT test was performed to determine proliferation activity of the cells and morphological analysis was carried out by phase-contrast microscopy. Our results show inhibitory effect of DMSO on WM-266-4 cells' metabolic activity. Morphology of the cells changed progressively with the time of exposure. Ethanol showed little effect on metabolic activity of the cells and no effect on cell morphology after selected period of time. According to our study, for specific in vitro tests concentrations of DMSO up to 1.5% may be appropriate for WM-266-4 cell line experiments.

Keywords

Dimethyl sulphoxide Melanoma cell line Metabolic activity Morphology MTT.

Ferk, P., & Dariš, B. (2018). The influence of dimethyl sulfoxide (DMSO) on metabolic activity and morphology of melanoma cell line WM-266-4. Cellular and Molecular Biology, 64(11), 41–43. https://doi.org/10.14715/cmb/2018.64.11.8

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References

Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. Multidisciplinary utilization of dimethyl sulfoxide: Pharmacological, cellular, and molecular aspects. Biochem Pharmacol 2003; 65:1035-41.
Qi W., Ding D, Salvi RJ. Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures. Hear Res 2008; 236: 52-60.
Hebling J, Bianchi L, Basso FG, Scheffel DL, Soares DG, Carrilho MRO et al. Cytotoxicity of dimethyl sulfoxide (DMSO) in direct contact with odontoblast-like cells. Dent Mater 2015; 31: 399-405.
Hollebeeck S, Raas T, Piront N, Schneider YJ, Toussaint O, Larondelle Y et al. Dimethyl sulfoxide (DMSO) attenuates the inflammatory response in the in vitro intestinal Caco-2 cell model. Toxicol Lett 2011; 206: 268-75.
Galvao J, Davis B, Tilley M, Normando E, Duchen MR, Cordeiro MF. Unexpected low-dose toxicity of the universal solvent DMSO. FASEB J 2014; 28: 1317-30.
Yi X, Liu M, Luo Q, Zhuo H, Cao H, Wang J et al. Toxic effects of dimethyl sulfoxide on red blood cells, platelets, and vascular endothelial cells in vitro. FEBS Open Bio 2017; 7: 485-94.
Barretina J, Caponigro G, Stransky N, Venkatesan K, Margolin AA, Kim S et al. The cancer cell line encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature 2012; 483: 603-7.
Li J, Zhao W, Akbani R, Liu W, Ju Z, Ling S et al. Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays. Cancer Cell 2017; 31: 225-239.
Sennoune SR, Santos JM, Hussain F, Martínez-Zaguilán R. Sodium calcium exchanger operates in the reverse mode in metastatic human melanoma cells. Cell Mol Biol 2015; 61: 40-9.
Somasundaram R, Zhang G, Fukunaga-Kalabis M, Perego M, Krepler C, Xu X, et al. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance. Nat Commun 2017; 8: 607.
Prunk Zdravkovic T, Zdravkovic B, Zdravkovic M, Daris B, Lunder M, Ferk P. In vitro study of the influence of octocrylene on a selected metastatic melanoma cell line. G Ital Dermatol Venereol 2017; Jul 11.
Lee H and Park JB. Evaluation of the effects of dimethylsulphoxide on morphology, cellular viability, mRNA, and protein expression of stem cells culture in growth media. Biomed Rep 2017; 7: 291-6.

References

Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. Multidisciplinary utilization of dimethyl sulfoxide: Pharmacological, cellular, and molecular aspects. Biochem Pharmacol 2003; 65:1035-41.

Qi W., Ding D, Salvi RJ. Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures. Hear Res 2008; 236: 52-60.

Hebling J, Bianchi L, Basso FG, Scheffel DL, Soares DG, Carrilho MRO et al. Cytotoxicity of dimethyl sulfoxide (DMSO) in direct contact with odontoblast-like cells. Dent Mater 2015; 31: 399-405.

Hollebeeck S, Raas T, Piront N, Schneider YJ, Toussaint O, Larondelle Y et al. Dimethyl sulfoxide (DMSO) attenuates the inflammatory response in the in vitro intestinal Caco-2 cell model. Toxicol Lett 2011; 206: 268-75.

Galvao J, Davis B, Tilley M, Normando E, Duchen MR, Cordeiro MF. Unexpected low-dose toxicity of the universal solvent DMSO. FASEB J 2014; 28: 1317-30.

Yi X, Liu M, Luo Q, Zhuo H, Cao H, Wang J et al. Toxic effects of dimethyl sulfoxide on red blood cells, platelets, and vascular endothelial cells in vitro. FEBS Open Bio 2017; 7: 485-94.

Barretina J, Caponigro G, Stransky N, Venkatesan K, Margolin AA, Kim S et al. The cancer cell line encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature 2012; 483: 603-7.

Li J, Zhao W, Akbani R, Liu W, Ju Z, Ling S et al. Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays. Cancer Cell 2017; 31: 225-239.

Sennoune SR, Santos JM, Hussain F, Martínez-Zaguilán R. Sodium calcium exchanger operates in the reverse mode in metastatic human melanoma cells. Cell Mol Biol 2015; 61: 40-9.

Somasundaram R, Zhang G, Fukunaga-Kalabis M, Perego M, Krepler C, Xu X, et al. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance. Nat Commun 2017; 8: 607.

Prunk Zdravkovic T, Zdravkovic B, Zdravkovic M, Daris B, Lunder M, Ferk P. In vitro study of the influence of octocrylene on a selected metastatic melanoma cell line. G Ital Dermatol Venereol 2017; Jul 11.

Lee H and Park JB. Evaluation of the effects of dimethylsulphoxide on morphology, cellular viability, mRNA, and protein expression of stem cells culture in growth media. Biomed Rep 2017; 7: 291-6.

Author biographies is not available.