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Hydro-thermal priming enhance seed germination capacity and seedling growth in sugar beet
Corresponding Author(s) : Mansour Taghvaei
Cellular and Molecular Biology,
Vol. 65 No. 4: Issue 4
Abstract
Seed priming improves seed performance in many crop species. In this study, the influence of hydrothermal priming on seed parameters of sugar beet is investigated in both laboratory and field conditions. In the laboratory, the treatments consist of a combination of cultivars (Arya and Shokoofa), hydro-priming at two temperatures (10 and 15 °C) for 6, 10, 14, 18, 22 hours. Germination traits and seedling growth were measured for determination of optimum hydro-thermal priming. Also, the protein pattern in the optimum hydro-thermal priming treatments and unprimed seeds were compared by electrophoresis. In the field experiment, the percentage and rate of emergence of primed and unprimed seeds were measured. Results showed that hydro-thermal priming had a positive effect on final germination percentage, mean germination time and uniformity of germination. Optimum hydro-thermal priming time and the temperature were 6 and 10 hours at 15 °C for Shokoofa and Arya cultivars respectively. Hydro-thermal priming increased the seed emergence percentage in the field by 15%. There was no significant difference in protein pattern between primed and unprimed seeds. In general, hydro-thermal priming not only increases sugar beet seed germination in the laboratory but also has a more positive effect on the emergence in the field condition.
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- Capron I, Corbineau F, Dacher F, Job C, Come D, Job D. Sugar beet seed priming: Effects of priming conditions on germination. Solubilization of 11-S globulin accumulation of LEA proteins. Seed Science Research 2000; 10:243-254.
- Tolman B, Stout M. Toxic effect on germinating sugar beet seed of water soluble substances in the seed ball. J Agric Res 1940; 61(11): 817-830.
- Dell Aquila A, Tritto V. Germination and biochemical activities in wheat seeds following delayed harvesting, ageing and osmotic priming. Seed Sci. and Technol 1991; 19:73-82.
- Donaldson E, Schillinger WF, Stephen MD. Straw production and grain yield relationships in winter wheat. Crop Sci 2001; 41:100 106.
- Bewley JD, Black M. Physiology and biochemistry of seeds in relation to germination. Springer-Verlag, Berlin, Germany 1982.
- Mubshar H, Farooq M, Basra SMA, Ahmad N. Influence of seed priming techniques on the seedling establishment, yield and quality of hybrid Sunflower. Int. J. Agri. Biol 2006; 8(1): 14-18.
- Bewley JD. Seed germination and dormancy. Plant Cell 1997; 9:1055-1066.
- Varier A, Vari AK, Dadlani M. The subcellular basis of seed priming. Curr. Sci 2010; 99(4): 450-456.
- Longden PC. Washing sugar beet seed. Journal of International Institute for Sugar Beet Research 1973; 6:154-162.
- Heydecker W, Chetram RS, Heydecker JC. Water relations of beet root seed germination. II. Effects of the ovary cap and of the endogenous inhibitors. Annals of Botany 1971; 35:31-42.
- Longden PC. Advanced sugar beet seed. Journal of Agricultural Science. Cambridge. 1971; 77: 43-46.
- Akeson WR, Henson MA, Ferytag AH, Westfall DG. Sugar beet fruit germination and emergence under moisture and temperature stress. Crop Sci 1980; 20: 735-739.
- Lanteri S, Belletii P, Marzach C, Nada E, Quagliotii L, Bino RJ. Priming- induced nuclear replication activity in pepper (Capsicum annum L.) seeds. Effect on germination and storability. In: Basic and Applied Aspects of Seed Biology, Ellis RH, Black M, Hong TD. (eds). Kluwer Academic Publisher, Dodrecht 1997; 451-459.
- McDonald MB. Seed priming. In Black M and Bewley JD (eds.), Seed technology and its biological basis. Sheffield Academic Press Ltd., Sheffield, England 2000; pp: 287-325.
- Gurusinghe SH, Cheng Z, Bradford KJ. Cell cycle activity during seed priming is not essential for germination advancement in tomato. Journal of Experimental Botany 1999; 50:101-106.
- Sliwinska E, Sadowski H. Comparison of the effects of bioactive compounds and hydration treatments on germination and cell cycle activity of sugar-beet seeds. Seed Sci. and Technol 2004; 32(3): 847-850.
- Camps G, Vernetti Fde-J, Augustin E, Irigon D. Morphological and electrophoretical characterization of twenty soybean cultivars. Pesqui. Agropecu. Bras 1994; 29(11):1779-1787.
- Jha SS, Ohri D. Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeon pea) and its wild relatives based on seed protein profiles. Genet. Resour. Crop Evol 1996; 43:275–281.
- Kakaei M, Zebarjadi AR, Mostafaie A. Study of protein pattern in Brassica napus genotypes under non-stress and drought stress conditions. Agricultural Biotechnology 2010; 9(2): 49-57.
- Bushehri SAA, Abd-Mishani C, Yazdi-Samadi B, Sayed-Tabatabaei BE. Variety specific electrophoretic profiles of soybean cultivars. Iranian J. Agric. Sci 2000; 31(1):55-61.
- Chiu KY, Chen CL, Sung JM. Effect of priming temperature on storability of primed sh-2 sweet corn seed. Crop Sci 2002; 42:1996-2003.
- Rush CM. Stand establishment of sugar beet seedling in pathogen-infested soils as influenced by cultivar and seed-priming technique. Plant Dis 1992; 76: 800-805.
- Thornton JM, Powell AA. Short-term aerated hydration for the improvement of seed quality in Brassica oleracea L. Seed Sci. Res 1992; 2: 41-49.
- ISTA. International rules for seed testing. International Seed Testing Association (ISTA), Seed Sci. and Technol 1999; 27, Supplement.
- ISTA. Hand book for seedling evaluation. International Seed Testing Association, Zurich, Switzerland 1993.
- Foti S, Cosentino SL, Patane C, Dagosta GM. Effect of osmo conditioning upon seed germination sorghum (Sorghom bicolor) Moench under low temperatures. Seed Sci. and Technol 2002; 30:521-533.
- Jalink H, Van Der Schoor IR. Seed Calcular 2.2 User's Guide. Report10. Plant Research International, Wageningen 2000; p. 12.
- Li CC. Introduction to experimental statistics. McGraw Hill Book Company. New York, USA 1964.
- Association of Official Seed Analysts (AOSA). Seed Vigor Testing Handbook. Contribution No.32, Lincoln, NE, USA: Association of Official Seed Analysts 1983.
- Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680-685.
- Mostafaie A. Theoretical and practical guide protein electrophoresis in gel. Kermanshah: Yadavaran Press 2003.
- Wang HY, Chen CL, Sung JM. Both warm water soaking and solid priming treatments enhance anti-oxidation of bitter gourd seeds germinated at sub-optimal temperature. Seed Sci. and Technol 2003; 31: 47-56.
- Bose B, Mishra T. Response of wheat seed to pre sowing seed treatments with Mg (NO3). Ann. Agric. Res. 1992; 13: 132-136.
- Jowkar M, Ghanbari A, Moradi F, Heidari M. Alterations in seed vigor and antioxidant enzymes activities in Silybum marianum under seed priming with KNO3. J. Med. Plants Res 2012; 6(7): 1176-1180.
- Taylor AG, Harman GE. Concepts and technologies of selected seed treatments. Annu Rev Phytopathol 1990; 28: 321-339.
- Ahmadi A, Mardeh AS, Poustini K, Jahromi ME. Influence of osmo and hydropriming on seed germination and seedling growth in wheat (Triticum aestivum L.) cultivars under different moisture and temperature conditions. Pak. J. Biol. Sci 2007; 10(22): 4043-4049.
- Noorbakhshian SJ, Nabipour M, Meskarbashee M, Amooaghaie R. Evaluation of germination and biochemical indexes of sainfoin hydroprimed seed. Am-Euras. J. Agric. Environ. Sci 2011a; 10(5): 671-678.
- Penalosa A, Eira M. Hydration dehydration treatment on tomato seeds (Lycopersicon esculentum Mill). Seed Sci. and Technol 1993; 21: 309-316.
- Wattanakulpakin P, Photchanachai S, Ratanakhanokchai K, Kyu KL, Ritthichai P, Miyagawa S. Hydropriming effects on carbohydrate metabolism, antioxidant enzyme activity and seed vigor of maize (Zea mays L.). Afr. J. Biotechnol 2012; 11(15): 3537-3547.
- Judi M, Sharifzadeh F. Investigation of hydro-priming Effects on barley cultivars. Desert 2006; 11:99-109. (In Farsi).
- Ghasemi- Golezani K, Sheikhzadeh- Mosaddegh P, Valizadeh M. Effects of hydropriming duration and limited irrigation on field performance of chickpea. Res. J. Seed Sci 2008; 1(1): 34-40.
- Abbasdokht H. The effect of hydro-priming and halo-priming on germination and early germination stage of wheat (Triticum aestivum L.). Desert 2011; 16:61-68.
- Rebetzke GJ, Botwright TL, Moore CS, Richardsand RA, Condon AG. Genotypic variation in specific leaf area for genetic improvement of early vigor in wheat. Field Crops Res 2004; 88(2): 179–189.
- Richards RA, Lukacs Z. Seedling vigour in wheat: Sources of variation for genetic and agronomic improvement. Aust. J. Agric Research Science 2002; 53: 41-50.
- Wang YR, Yu L, Nan ZB. Use of seed vigor tests to predict field emergence of lucerne (Medicago sativa). New Zeal J Agr Res 1996; 39: 255-262.
- Rehman HU, Basra SMA, Farooq M. Field appraisal of seed priming to improve the growth, yield, and quality of direct seeded rice. Turk J. Agric 2011; 35: 357-365.
- Li Y, Huang SZ, Fu JR. One and two-dimensional polyacrylamide gel electrophoretic analysis of seed polypeptide composition of peanut cultivars. Acta Botanica Sinica 1998; 40(6): 534-541.
- Zarei I, Sohrabi S, Kahrizi D, Yari K. Effect of different hydropriming times on the quantitative and qualitative characteristics of chickpea (Cicer arietinum L.). Afr J Biotechnol 2011; 10(66): 14844-14850.
References
Capron I, Corbineau F, Dacher F, Job C, Come D, Job D. Sugar beet seed priming: Effects of priming conditions on germination. Solubilization of 11-S globulin accumulation of LEA proteins. Seed Science Research 2000; 10:243-254.
Tolman B, Stout M. Toxic effect on germinating sugar beet seed of water soluble substances in the seed ball. J Agric Res 1940; 61(11): 817-830.
Dell Aquila A, Tritto V. Germination and biochemical activities in wheat seeds following delayed harvesting, ageing and osmotic priming. Seed Sci. and Technol 1991; 19:73-82.
Donaldson E, Schillinger WF, Stephen MD. Straw production and grain yield relationships in winter wheat. Crop Sci 2001; 41:100 106.
Bewley JD, Black M. Physiology and biochemistry of seeds in relation to germination. Springer-Verlag, Berlin, Germany 1982.
Mubshar H, Farooq M, Basra SMA, Ahmad N. Influence of seed priming techniques on the seedling establishment, yield and quality of hybrid Sunflower. Int. J. Agri. Biol 2006; 8(1): 14-18.
Bewley JD. Seed germination and dormancy. Plant Cell 1997; 9:1055-1066.
Varier A, Vari AK, Dadlani M. The subcellular basis of seed priming. Curr. Sci 2010; 99(4): 450-456.
Longden PC. Washing sugar beet seed. Journal of International Institute for Sugar Beet Research 1973; 6:154-162.
Heydecker W, Chetram RS, Heydecker JC. Water relations of beet root seed germination. II. Effects of the ovary cap and of the endogenous inhibitors. Annals of Botany 1971; 35:31-42.
Longden PC. Advanced sugar beet seed. Journal of Agricultural Science. Cambridge. 1971; 77: 43-46.
Akeson WR, Henson MA, Ferytag AH, Westfall DG. Sugar beet fruit germination and emergence under moisture and temperature stress. Crop Sci 1980; 20: 735-739.
Lanteri S, Belletii P, Marzach C, Nada E, Quagliotii L, Bino RJ. Priming- induced nuclear replication activity in pepper (Capsicum annum L.) seeds. Effect on germination and storability. In: Basic and Applied Aspects of Seed Biology, Ellis RH, Black M, Hong TD. (eds). Kluwer Academic Publisher, Dodrecht 1997; 451-459.
McDonald MB. Seed priming. In Black M and Bewley JD (eds.), Seed technology and its biological basis. Sheffield Academic Press Ltd., Sheffield, England 2000; pp: 287-325.
Gurusinghe SH, Cheng Z, Bradford KJ. Cell cycle activity during seed priming is not essential for germination advancement in tomato. Journal of Experimental Botany 1999; 50:101-106.
Sliwinska E, Sadowski H. Comparison of the effects of bioactive compounds and hydration treatments on germination and cell cycle activity of sugar-beet seeds. Seed Sci. and Technol 2004; 32(3): 847-850.
Camps G, Vernetti Fde-J, Augustin E, Irigon D. Morphological and electrophoretical characterization of twenty soybean cultivars. Pesqui. Agropecu. Bras 1994; 29(11):1779-1787.
Jha SS, Ohri D. Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeon pea) and its wild relatives based on seed protein profiles. Genet. Resour. Crop Evol 1996; 43:275–281.
Kakaei M, Zebarjadi AR, Mostafaie A. Study of protein pattern in Brassica napus genotypes under non-stress and drought stress conditions. Agricultural Biotechnology 2010; 9(2): 49-57.
Bushehri SAA, Abd-Mishani C, Yazdi-Samadi B, Sayed-Tabatabaei BE. Variety specific electrophoretic profiles of soybean cultivars. Iranian J. Agric. Sci 2000; 31(1):55-61.
Chiu KY, Chen CL, Sung JM. Effect of priming temperature on storability of primed sh-2 sweet corn seed. Crop Sci 2002; 42:1996-2003.
Rush CM. Stand establishment of sugar beet seedling in pathogen-infested soils as influenced by cultivar and seed-priming technique. Plant Dis 1992; 76: 800-805.
Thornton JM, Powell AA. Short-term aerated hydration for the improvement of seed quality in Brassica oleracea L. Seed Sci. Res 1992; 2: 41-49.
ISTA. International rules for seed testing. International Seed Testing Association (ISTA), Seed Sci. and Technol 1999; 27, Supplement.
ISTA. Hand book for seedling evaluation. International Seed Testing Association, Zurich, Switzerland 1993.
Foti S, Cosentino SL, Patane C, Dagosta GM. Effect of osmo conditioning upon seed germination sorghum (Sorghom bicolor) Moench under low temperatures. Seed Sci. and Technol 2002; 30:521-533.
Jalink H, Van Der Schoor IR. Seed Calcular 2.2 User's Guide. Report10. Plant Research International, Wageningen 2000; p. 12.
Li CC. Introduction to experimental statistics. McGraw Hill Book Company. New York, USA 1964.
Association of Official Seed Analysts (AOSA). Seed Vigor Testing Handbook. Contribution No.32, Lincoln, NE, USA: Association of Official Seed Analysts 1983.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680-685.
Mostafaie A. Theoretical and practical guide protein electrophoresis in gel. Kermanshah: Yadavaran Press 2003.
Wang HY, Chen CL, Sung JM. Both warm water soaking and solid priming treatments enhance anti-oxidation of bitter gourd seeds germinated at sub-optimal temperature. Seed Sci. and Technol 2003; 31: 47-56.
Bose B, Mishra T. Response of wheat seed to pre sowing seed treatments with Mg (NO3). Ann. Agric. Res. 1992; 13: 132-136.
Jowkar M, Ghanbari A, Moradi F, Heidari M. Alterations in seed vigor and antioxidant enzymes activities in Silybum marianum under seed priming with KNO3. J. Med. Plants Res 2012; 6(7): 1176-1180.
Taylor AG, Harman GE. Concepts and technologies of selected seed treatments. Annu Rev Phytopathol 1990; 28: 321-339.
Ahmadi A, Mardeh AS, Poustini K, Jahromi ME. Influence of osmo and hydropriming on seed germination and seedling growth in wheat (Triticum aestivum L.) cultivars under different moisture and temperature conditions. Pak. J. Biol. Sci 2007; 10(22): 4043-4049.
Noorbakhshian SJ, Nabipour M, Meskarbashee M, Amooaghaie R. Evaluation of germination and biochemical indexes of sainfoin hydroprimed seed. Am-Euras. J. Agric. Environ. Sci 2011a; 10(5): 671-678.
Penalosa A, Eira M. Hydration dehydration treatment on tomato seeds (Lycopersicon esculentum Mill). Seed Sci. and Technol 1993; 21: 309-316.
Wattanakulpakin P, Photchanachai S, Ratanakhanokchai K, Kyu KL, Ritthichai P, Miyagawa S. Hydropriming effects on carbohydrate metabolism, antioxidant enzyme activity and seed vigor of maize (Zea mays L.). Afr. J. Biotechnol 2012; 11(15): 3537-3547.
Judi M, Sharifzadeh F. Investigation of hydro-priming Effects on barley cultivars. Desert 2006; 11:99-109. (In Farsi).
Ghasemi- Golezani K, Sheikhzadeh- Mosaddegh P, Valizadeh M. Effects of hydropriming duration and limited irrigation on field performance of chickpea. Res. J. Seed Sci 2008; 1(1): 34-40.
Abbasdokht H. The effect of hydro-priming and halo-priming on germination and early germination stage of wheat (Triticum aestivum L.). Desert 2011; 16:61-68.
Rebetzke GJ, Botwright TL, Moore CS, Richardsand RA, Condon AG. Genotypic variation in specific leaf area for genetic improvement of early vigor in wheat. Field Crops Res 2004; 88(2): 179–189.
Richards RA, Lukacs Z. Seedling vigour in wheat: Sources of variation for genetic and agronomic improvement. Aust. J. Agric Research Science 2002; 53: 41-50.
Wang YR, Yu L, Nan ZB. Use of seed vigor tests to predict field emergence of lucerne (Medicago sativa). New Zeal J Agr Res 1996; 39: 255-262.
Rehman HU, Basra SMA, Farooq M. Field appraisal of seed priming to improve the growth, yield, and quality of direct seeded rice. Turk J. Agric 2011; 35: 357-365.
Li Y, Huang SZ, Fu JR. One and two-dimensional polyacrylamide gel electrophoretic analysis of seed polypeptide composition of peanut cultivars. Acta Botanica Sinica 1998; 40(6): 534-541.
Zarei I, Sohrabi S, Kahrizi D, Yari K. Effect of different hydropriming times on the quantitative and qualitative characteristics of chickpea (Cicer arietinum L.). Afr J Biotechnol 2011; 10(66): 14844-14850.