Issue

Vol. 66 No. 5: Issue 5

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Importance of miR-125a-5p and miR-122-5p expression in patients with HBV infection

https://doi.org/10.14715/cmb/2020.66.5.1
Ramin Lak
Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran.
Ramin Yaghobi
Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Masood Garshasbi
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Corresponding Author(s) : Ramin Yaghobi

rayaviro@yahoo.com

Cellular and Molecular Biology, Vol. 66 No. 5: Issue 5

Abstract

MicroRNAs (miRNAs) as a small RNA and post-transcriptional modulator are shown to have regulatory effects for different cellular activities and pathways, such as metabolism, virus replication and also cell growth. In addition, miRNAs can regulate the replication of the hepatitis B virus (HBV). Therefore, the expression profile of miRNAs was evaluated in HBV infected patient groups and healthy controls. The expression levels of following microRNAs (as noninvasive biomarkers) were compared in two experimental (those with various stages of HBV infection) and control groups to evaluate their diagnosis ability: miR-122-5p, miR-125a-5p, miR-199a-3p, miR-210-5p, miR-205-5p, miR-155-5p, miR-372-5p, and miR-1-5p. RNA extraction was performed for 45 serum samples. The miRCURY LNAâ„¢ Universal RT-miRNA-PCR system and miRNA PCR panels were used for measuring microRNA expression profiles. To normalize quantitative values, the endogenous reference by UniSp6 expression was used.Serum miR-125a-5p and miR-122-5p were significantly higher in patients in different stages of HBV infection (p<0.001) than in controls (p<0.001). Receiver operating characteristic (ROC) curve analyses suggested that serum miR-125a-5p and miR-122-5p have significant diagnostic value for HBV infection. A significant difference was not found in terms of serum levels of other miRs (miR-199a-3p, miR-210-5p, miR-205-5p, miR-155-5p, miR-372-5p and miR-1-5p). Results suggest that miR-125a-5p and miR-122-5p can be used as possible noninvasive biomarkers for monitoring of HBV infection need to confirm in future completed studies.

Keywords

MicroRNAs Hepatitis B Virus Biomarker
Lak, R., Yaghobi, R., & Garshasbi, M. (2020). Importance of miR-125a-5p and miR-122-5p expression in patients with HBV infection. Cellular and Molecular Biology, 66(5), 1–8. https://doi.org/10.14715/cmb/2020.66.5.1
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. MacLachlan JH, Cowie BC. Hepatitis B virus epidemiology. Cold Spring Harb Perspect Med 2015;5.
  2. Liang TJ. Hepatitis B: The virus and disease. Hepatology 2009;49: S13–21.
  3. Farokhizadeh Z, Dehbidi S, Geramizadeh B, Yaghobi R, Malekhosseini SA, Behmanesh M, et al. Association of MicroRNA Polymorphisms With Hepatocellular Carcinoma in an Iranian Population. Ann Lab Med 2019;39:58.
  4. Wang G, Dong F, Xu Z, Sharma S, Hu X, Chen D, et al. MicroRNA profile in HBV-induced infection and hepatocellular carcinoma. BMC Cancer 2017;17:805.
  5. Khoshtinat Nikkhoi S, Dorostkar R, Ranjbar S, Heydarzadeh H, Tat M, Ghalavand M, Farasat A, Hashemzadeh MS. Synergistic Effect of Expressed miR-128 and Puma Protein on Targeted Induction of Tumor Cell Apoptosis. Iran J Biotechnol. 2016; 14(3): 185-191.
  6. Skalsky RL, Cullen BR. Viruses, microRNAs, and Host Interactions. Annu Rev Microbiol. 2010;64:123–41.
  7. Naqvi AR, Shango J, Seal A, Shukla D, Nares S. Viral miRNAs alter host cell miRNA profiles and modulate innate immune responses. Front Immunol 2018;9:433.
  8. Bernier A, Sagan SM. The diverse roles of microRNAs at the host-virus interface. Viruses. 2018; 10:440.
  9. Onorato L, Russo A, Sagnelli C, Sagnelli E, Potenza N, Coppola N. Micro-RNAs in hepatitis B virus-related chronic liver diseases and hepatocellular carcinoma. World J Hepatol 2018;10:558–70.
  10. Wang Z, Wu Z, Huang P. The function of miRNAs in hepatocarcinogenesis induced by hepatitis B virus X protein. Oncol Rep 2017;38:652–64.
  11. Zhang G ling, Li Y Xuan, Zheng S qi, Liu M, Li X, Tang H. Suppression of hepatitis B virus replication by microRNA-199a-3p and microRNA-210. Antiviral Res 2010;88:169–75.
  12. Zhang X, Zhang E, Ma Z, Pei R, Jiang M, Schlaak JF, et al. Modulation of hepatitis B virus replication and hepatocyte differentiation by MicroRNA-1. Hepatology 2011;53:1476–1485.
  13. Jiang X, Kanda T, Wu S, Nakamura M, Miyamura T, Nakamoto S, et al. Regulation of microRNA by hepatitis B virus infection and their possible association with control of innate immunity. World J Gastroenterol 2014;20:7197–06.
  14. Zhu H, Geng Y, He Q, Li M. miRNAs regulate immune response and signaling during hepatitis C virus infection. Eur J Med Res 2018;23:19.
  15. Kitab B, Alj Hs, Ezzikouri S, Benjellou S. MicroRNAs as Important Players in Host-hepatitis B Virus Interactions. J Clin Transl Hepatol 2015;3:141-61.
  16. Fu Y, Wei X, Tang C, Li J, Liu R, Shen A, et al. Circulating microRNA-101 as a potential biomarker for hepatitis B virus-related hepatocellular carcinoma. Oncol Lett 2013;6:1811–15.
  17. Su C, Hou Z, Zhang C, Tian Z, Zhang J. Ectopic expression of microRNA-155 enhances innate antiviral immunity against HBV infection in human hepatoma cells. Virol J 2011;8:354.
  18. Zamor P, Delemos A, Gastrointestinal MR-J of, 2017 U. Viral hepatitis and hepatocellular carcinoma: Etiology and Management. J Gastrointest Oncol 2017;8:229–42.
  19. Behzad-Behbahani A, Mojiri A, Tabei SZ, Farhadi-Andarabi A, Pouransari R, Yaghobi R, et al. Outcome of hepatitis B and C virus infection on graft function after renal transplantation. Transplant Proc 2005;37:3045–47.
  20. Potenza N, Papa U, Mosca N, Zerbini F, Nobile V, Russo A. Human microRNA hsa-miR-125a-5p interferes with expression of hepatitis B virus surface antigen. Nucleic Acids Res 2011;39:5157–63.
  21. Liu Y, Wang C-M, Han P, Wang L, Cheng Y-Q, Xu D-P, et al. Altered expression profiles of microRNAs in a stable hepatitis B virus-expressing cell line. Chin Med J (Engl) 2009;122:10–4.
  22. Park SO, Kumar M, Gupta S. TGF-β and Iron Differently Alter HBV Replication in Human Hepatocytes through TGF-β/BMP Signaling and Cellular MicroRNA Expression. Bouchard M, editor. PLoS One 2012;7:e39276.
  23. Le Duff Y, Blanchet M, Sureau C. The Pre-S1 and Antigenic Loop Infectivity Determinants of the Hepatitis B Virus Envelope Proteins Are Functionally Independent. J Virol 2009;83:12443–51.
  24. Musaddaq G, Shahzad N, Ashraf MA, Arshad MI. Circulating liver-specific microRNAs as noninvasive diagnostic biomarkers of hepatic diseases in human. Biomarkers 2019;24:103–9.
  25. Ebrahimifard M, Zandi M, Moradi A. Evaluation of miR-122 level in chronic HBV infection and liver cirrhosis patients. Arch Med Lab Sci 2016;2:108-10.
  26. Zhou J, Yu L, Gao X, Hu J, Wang J, Dai Z, et al. Plasma microRNA panel to diagnose hepatitis B virus-related hepatocellular carcinoma. J Clin Oncol 2011;29:4781–8.
  27. Bandopadhyay M, Sarkar N, Datta S, Das D, Pal A, Panigrahi R, et al. Hepatitis B virus X protein mediated suppression of miRNA-122 expression enhances hepatoblastoma cell proliferation through cyclin G1-p53 axis. Infect Agent Cancer 2016;11:40.
  28. Oda S, Takeuchi M, Akai S, Shirai Y, Tsuneyama K, Yokoi T. miRNA in Rat Liver Sinusoidal Endothelial Cells and Hepatocytes and Application to Circulating Biomarkers that Discern Pathogenesis of Liver Injuries. Am J Pathol 2018;188:916–28.
  29. Thirion M, Ochiya T. Roles of microRNAs in the hepatitis B virus infection and related diseases. Viruses 2013;5:2690–703.
  30. Mizuguchi Y, Takizawa T, Uchida E. Host cellular microRNA involvement in the control of hepatitis B virus gene expression and replication. World J Hepatol 2015;7:696–02.
  31. Ghanbari R, Ravanshad M, Hosseini SY, Yaghobi R, Shahzamani K. Genotyping and infection rate of GBV-C among Iranian HCV-infected patients. Hepat Mon 2010;10:80–7.
  32. Zhang T, Zhang J, Cui M, Liu F, You X, Du Y, et al. Hepatitis B Virus X Protein Inhibits Tumor Suppressor miR-205 through Inducing Hypermethylation of miR-205 Promoter to Enhance Carcinogenesis. Neoplasia 2015;15:1282-91.
  33. Sarkar N, sciences RC-I journal of molecular, undefined. Hepatitis B virus infection, microRNAs and liver disease. Int J Mol Sci 2015;16:17746–62.
  34. Guo H, Liu H, Mitchelson K, Rao H, Luo M, Xie L, et al. MicroRNAs-372/373 promote the expression of hepatitis B virus through the targeting of nuclear factor I/B. Hepatology 2011;54:808–19.
  35. Bordbar M, Darvishzadeh R, Pazhouhandeh M, Kahrizi D. An overview of genome editing methods based on endonucleases. Modern Genetics J 2020; 15(2): 75-92.
  36. Ghaheri M, Kahrizi D, Bahrami G, Mohammadi-Motlagh HR. Study of gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni under various mannitol concentrations. Mol Biol Rep 2019; 46(1): 7-16.
  37. Eruygur N, Ucar E, Akpulat HA, Shahsavari K, Safavi SM, Kahrizi D. In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium. Mol Biol Rep 2019; 46(2): 2121-9.
  38. Esmaeili F, Ghaheri M, Kahrizi D, Mansouri M, Safavi SM, Ghorbani T, Muhammadi S, Rahmanian E, Vaziri S. Effects of various glutamine concentrations on gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 1-5.
  39. Akbari F, Arminian A, Kahrizi D, Fazeli A, Ghaheri M. Effect of nitrogen sources on gene expression of Stevia rebaudiana (Bertoni) under in vitro conditions. Cell Mol Biol, 64(2): 11-16.
  40. Ghaheri M, Adibrad E, Safavi SM, Kahrizi D, Soroush A, Muhammadi S, Ghorbani T, Sabzevari A, Ansarypour Z, Rahmanian E. Effects of life cycle and leaves location on gene expression and glycoside biosynthesis pathway in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 17-22.
  41. Kahrizi D, Ghaheri M, Yari Z, Yari K, Bahraminejad S. Investigation of different concentrations of MS media effects on gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 23-27.
  42. Hashempoor S, Ghaheri M, Kahrizi D, Kazemi N, Muhammadi S, Safavi SM, Ghorbani T, Rahmanian E, Heshmatpanaah M. Effects of different concentrations of mannitol on gene expression in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 28-31.
  43. Akbarabadi A, Ismaili A, Kahrizi D, Firouzabadi FN. Validation of expression stability of reference genes in response to herbicide stress in wild oat (Avena ludoviciana). Cell Mol Biol 2018; 64(4). 113-118.
  44. Ghorbani T, Kahrizi D, Saeidi M, Arji I. Effect of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression. Cell Mol Biol, 63(11): 32-36.
  45. Kahrizi D, Ghari SM, Ghaheri M, Fallah F, Ghorbani T, Beheshti AA, Kazemi E, Ansarypour Z. Effect of KH2PO4 on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol, 63(7): 107-111.
  46. Fallah F, Nokhasi F, Ghaheri M, Kahrizi D, Beheshti Ale AA, Ghorbani T, Kazemi E, Ansarypour Z. Effect of salinity on gene expression, morphological and biochemical characteristics of Stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol 2017; 63(7): 102-106.
  47. Fatemi SS, Yari K, Tavallaei M, Kahrizi D. Expression of recombinant Hc domain of Clostridium botulinum neurotoxin A in E. coli and its purification as a vaccine candidate against botulism. Sci J Kurdistan Univ Med Sci 2011 Aug 10;16(2):36-44.
  48. Motamedi MJ, Shahsavandi S, Amani J, Kazemi R, Takrim S, Jafari M, Salmanian AH. Immunogenicity of the Multi-Epitopic Recombinant Glycoproteins of Newcastle Disease Virus: Implications for the Serodiagnosis Applications. Iran J Biotechnol. 2018; 16(4): e1749.
  49. Ali S, Ahmad Nasir I, Rafiq M, Javed Butt S, Ihsan F, Qayyum Rao A, Husnain T. Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana. Iran J Biotechnol. 2017; 15(4): 260-267.
  50. Chen T, Zou X, Xu Z, Wang Y, Wang P, Peng H, Liu D, Lin J, Luo R, Wang Y, Chen Q, Chen D, Cai M, Li M. Molecular Characterization of the Epstein-Barr Virus BGLF2 Gene, its Expression, and Subcellular Localization. Iran J Biotechnol. May 2018; 16(2): e1610.
  51. Rao X, Sun J. Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus. Iran J Biotechnol. Sep 2015; 13(3): 20-24.
  52. Savardashtaki A, Sharifi Z, Hamzehlou S, Farajollahi MM. Analysis of Immumoreactivity of Heterologously Expressed Non-structural Protein 4B (NS4B) from Hepatitis C Virus (HCV) Genotype 1a. Iran J Biotechnol. Dec 2015; 13(4): 32-37.
  53. Shafaghi M, Maktoobian S, Rasouli R, Howaizi N, Ofoghi H, Ehsani P. Transient Expression of Biologically Active Anti-rabies Virus Monoclonal Antibody in Tobacco Leaves. Iran J Biotechnol. Apr 2018; 16(1): e1774.
  54. Shibaei N, Majidi J, Bashir NS, Karkhaneh A, Razavi K. Production and Partial Purification of the Grapevine Fanleaf Virus Coat Protein 42 Polyclonal Antibody Against Inclusion Body Expressed in Escherichia coli. Iran J Biotechnol. Dec 2018; 16(4): e1525.
  55. Singh A, Permar V, Basavaraj, Tomar BS, Praveen S. Effect of Temperature on Symptoms Expression and Viral RNA Accumulation in Groundnut Bud Necrosis Virus Infected Vigna unguiculata. Iran J Biotechnol. Aug 2018; 16(3): e1846.
  56. Sokhandan Bashir N, Poorsmaile M, Hajizadeh M. Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187. Iran J Biotechnol. Dec 2015; 13(4): 48-52.
  57. Vafaei SH, Mahmoodi M. Presence of Recombinant Strain of Cucurbit Aphid Borne Yellows Virus in Iran. Iran J Biotechnol. 2017; 15(4): 289-295.
  58. Venkataravanappa V, Reddy CNL, Shankarappa KS, Reddy MK. Association of Tomato Leaf Curl New Delhi Virus, Betasatellite, and Alphasatellite with Mosaic Disease of Spine Gourd (Momordica dioica Roxb. Willd) in India. Iran J Biotechnol. Jan 2019; 17(1): e2134.
  59. Yan Y, Jiang W, Liu J, Xu W, Qian H. Expression of Recombinant Phosphodiesterases 3A and 3B Using Baculovirus Expression System. Iran J Biotechnol. Dec 2016; 14(4): 236-242.
  60. Zhang J, Jin Z, Sun T, Jiang Y, Han Q, Song Y, Chen Q, Xia X. Prokaryotic Expression, Purification, and Polyclonal Antibody Production of a Truncated Recombinant Rabies Virus L Protein. Iran J Biotechnol. Jun 2015; 13(2): 18-24.
  61. Zou X, Xu Z, Wang Y, Ou X, Li Y, Liu D, Gan W, Lu M, Chen Q, Peng H, Hong G, Lin J, Li M, Cai M. Expression, Purification, and Antiserum Production of the Truncated UL31 Protein of Herpes Simplex Virus 1. Iran J Biotechnol. Jan 2019; 17(1): e1609.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 998 Download : 557

Most read articles by the same author(s)