Influence of acupuncture on the expression of VIP, SP, NKA and NKB, cAMP/cGMP and HE content and treatment of bronchial asthma in rats

Yujuan Chen; Yanlu Gao; Wenwen Lu; Wei Gao

doi:10.14715/cmb/2020.66.5.6

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Vol. 66 No. 5: Issue 5

Issue Published : August 6, 2020

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Influence of acupuncture on the expression of VIP, SP, NKA and NKB, cAMP/cGMP and HE content and treatment of bronchial asthma in rats

https://doi.org/10.14715/cmb/2020.66.5.6

Yujuan Chen

Yanlu Gao

Wenwen Lu

Wei Gao

Corresponding Author(s) : Wei Gao

chinaacademicauthor2020@yahoo.com

Cellular and Molecular Biology, Vol. 66 No. 5: Issue 5
Article Published : July 31, 2020

Abstract

This research was set up to explore the neural mechanisms of acupuncture in the treatment of bronchial asthma in rats by detecting the content of substance P(SP), vasoactive intestinal peptide (VIP), neurokinin A(NKA), neurokinin B (NKB), cyclic adenosine monophosphate/cyclic guanosine monophosphate ratio (cAMP/cGMP) and hematoxylin-eosin (HE) staining for the pathological changes of lung tissue, in order to Institute Certain Experimental and Theoretical Foundation for Traditional Chinese Medicine (TCM) Prevention and Treatment of Bronchial Asthma. For this purpose, fifty healthy adult Wistar male rats, weighing 200-250 g, were randomly divided into 5 groups: normal control group A, asthma control group B, asthma acupuncture group C, adrenalectomy (ADX)-asthma group D, adrenalectomy (ADX)-asthma acupuncture group E. Group A was raised with other groups at the same period; Group B was induced asthma by ovalbumin; Group C was induced asthma as Group B and then acupunctured five acupoints (bilateral Feishu, bilateral Fengmen, and Dazhui); Group D was induced asthma after adrenalectomy; group E was treated with acupuncture on the basis of group D. HE staining was performed in the lung tissue of rats from each group, and histopathologic changes were observed. SP, VIP, NKA, NKB in each rat lung tissue were measured by immunohistochemistry. cAMP/cGMP was measured with ELISA to speculate the neural mechanisms of acupuncture in the treatment of bronchial asthma. The results were as: decrease of cAMP/cGMP and VIP and increase of SP, NKA, NKB in the lung tissue are the neural mechanisms of an asthma attack. The increase of cAMP/cGMP and decrease of NKA, NKB, SP and VIP in the lung tissue of group C indicated the improvement of bronchial asthma symptoms. It is possible that the decrease of NKA and NKB, increase of cAMP/cGMP and a slight change of SP and VIP in group E were related to the reduction of glucocorticoid after ADX which influenced the effect of acupuncture. The neural regulation mechanisms of acupuncture in the treatment of bronchial asthma were related to bronchiectasis caused by stimulation of adrenergic nerve and inhibition of the vagus nerve function by acupuncture, and related to the release of inflammatory mediators.

Keywords

Acupuncture Bronchial asthma Neural mechanism

Chen, Y., Gao, Y., Lu, W., & Gao, W. (2020). Influence of acupuncture on the expression of VIP, SP, NKA and NKB, cAMP/cGMP and HE content and treatment of bronchial asthma in rats. Cellular and Molecular Biology, 66(5), 29–35. https://doi.org/10.14715/cmb/2020.66.5.6

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References

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Li M, Yin K, Dong J. Pharmacotherapy asthma. Beijing: People's Medical Publishing House, 2001; 2-3, 6.
Van der Velden VH, Hulsmann AR. Autonomic innervation of human airways: structure, function, and pathophysiology in asthma. Neuroimmunomodulation, 1999; 6l145-159.
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Hou Z, Flash cupping Fengmen for treating repeated and long Coughing. Chin Trad Chin Med 2014; 21(5): 1-2.
Cao Xijun, Z, Zhaohe LW. Clinical application of Fengmen. J Bee,2007, 8(8): 45.
Yu S, Guo Y. Experimental Acupuncturology.Shanghai: Shanghai Sci Technol Publishing House 2009; 150-151.
Zhang Y, Chen Y, Yu H, Study on the time-effect of acupuncture on Feishu to improve lung function. Chinese Acupuncture & Moxibustion,1997, 17(10): 581-583.
Li W, Tan L, Miao L, Li P, Chen J. Effect of Electro-acupuncture of BL13 on Symptoms and Pulmonary Function in Asthma. J Clinic Acupuncture Moxibustion 2010; 26(1): 4-6.
Wang X. Discussion on the neural regulation mechanism of acupuncture and moxibustion in the treatment of bronchial asthma. Chin Famous Doctor Forum 2005; 3: 13-14.
Bordbar M, Darvishzadeh R, Pazhouhandeh M, Kahrizi D. An overview of genome editing methods based on endonucleases. Modern Genetics J 2020; 15(2): 75-92.

References

Ni W. Internal Medicine. Bei Jing: Chinese Medicine Publishing House, 2012; 8 28-33.

Li M, Yin K, Dong J. Pharmacotherapy asthma. Beijing: People's Medical Publishing House, 2001; 2-3, 6.

Van der Velden VH, Hulsmann AR. Autonomic innervation of human airways: structure, function, and pathophysiology in asthma. Neuroimmunomodulation, 1999; 6l145-159.

Qiu C. Modern Molecular Biological Technology and Asthma. Beijing: People's Medical Publishing House, 2012; 9, 19-20.

Said SI, VIP as a modulator of lung inflammation and airway constriction.Am Rev Respir Dis,1991; 143:522.

Barnes PJ. Airway neuropeptides and asthma.Trends Pharmol Sci 1987; 8:24.

Zhang X, Xia Y. Research on pathogenesis of bronchial asthma. Chin J Coal Indust Med 2010; 13(8): 1252-1254.

Chen W, Kong Q. Observation on immediate effect of patients with asthma by acupuncture Dazhui. Acupuncture Res1998; 23(3): 199-120.

Li X. Experimental study on the effect of acupuncture on Dazhui on immune function in asthmatic guinea pigs. Beijing University of Chinese Medicine, 2003, 5.

Zhang W, Chen M, Xiong J. Influence of moxibustion of Dazhui on IL-4 and INF-Î³ in serum in asthmatic rats. Chin J Basic Med in Trad Chin Med. 2012; 18(5): 541-542.

Hou Z, Flash cupping Fengmen for treating repeated and long Coughing. Chin Trad Chin Med 2014; 21(5): 1-2.

Cao Xijun, Z, Zhaohe LW. Clinical application of Fengmen. J Bee,2007, 8(8): 45.

Yu S, Guo Y. Experimental Acupuncturology.Shanghai: Shanghai Sci Technol Publishing House 2009; 150-151.

Zhang Y, Chen Y, Yu H, Study on the time-effect of acupuncture on Feishu to improve lung function. Chinese Acupuncture & Moxibustion,1997, 17(10): 581-583.

Li W, Tan L, Miao L, Li P, Chen J. Effect of Electro-acupuncture of BL13 on Symptoms and Pulmonary Function in Asthma. J Clinic Acupuncture Moxibustion 2010; 26(1): 4-6.

Wang X. Discussion on the neural regulation mechanism of acupuncture and moxibustion in the treatment of bronchial asthma. Chin Famous Doctor Forum 2005; 3: 13-14.

Bordbar M, Darvishzadeh R, Pazhouhandeh M, Kahrizi D. An overview of genome editing methods based on endonucleases. Modern Genetics J 2020; 15(2): 75-92.

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