Cisatracurium inhibits the proliferation, migration and invasion of breast cancer cells by regulating the expression of miR-3174

Xiaozhong Yang; Wenxia Ma

doi:10.14715/cmb/2020.66.6.1

Issue

Vol. 66 No. 6: Issue 6

Issue Published : October 6, 2020

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Cisatracurium inhibits the proliferation, migration and invasion of breast cancer cells by regulating the expression of miR-3174

https://doi.org/10.14715/cmb/2020.66.6.1

Xiaozhong Yang

Department of Pharmacy, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, 710038, China

Wenxia Ma

Department of Pharmacy, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, 710038, China

Corresponding Author(s) : Wenxia Ma

hu_duantan1972@163.com

Cellular and Molecular Biology, Vol. 66 No. 6: Issue 6
Article Published : September 30, 2020

Abstract

Breast cancer is a type of cancer that begins in the breast tissue. Being a woman is the most important factor in the risk of breast cancer. Although men also get the cancer, women are much more likely to get it. This experiment was founded to investigate the effect and mechanism of Cisatracurium on breast cancer cell proliferation, migration and invasion. Breast cancer cells MDA-MB-231 were cultured in vitro. MDA-MB-231 cells were treated with cisatracurium of different concentrations for 48 h. CCK-8method detected cell proliferation, Transwell detected cell migration and invasion, Western Blot method detected the expression levels of CyclinD1, p21, MMP-2andMMP-9protein in cells, RT-qPCR) detected the expression level of miR-3174in cells. After miR-3174 inhibitor was transfected into MDA-MB-231 in order to down-regulate the expression of miR-3174, the same methods as above were used to observe the effect of the down-regulating miR-3174 expression on MDA-MB-231 cell proliferation, migration and invasion as well as the expression levels of CyclinD1, p21, MMP -2 andMMP-9 protein. After different concentrations of Cisatracurium acted on MDA-MB-231 cells, the cell inhibition rate and p21 protein expression were significantly increased (p<0.05), the number of cell migration and invasion and the expression levels of CyclinD1, MMP-2 and MMP-9 were significantly reduced (p<0.05), and the expression of miR-3174 in cells was significantly reduced (p<0.05). After down-regulating the expression of miR-3174, the cell inhibition rate and p21 protein expression were significantly increased (p<0.05), the number of cell migration and invasion and the expression levels of CyclinD1, MMP-2 and MMP-9 were significantly reduced (p<0.05). Up-regulating miR-3174 expression could reverse the effect of Cisatracurium on the proliferation, migration and invasion of MDA-MB-231 cells. Cisatracurium can inhibit the proliferation, migration and invasion of breast cancer MDA-MB-231 cells, and its mechanism is related to the down-regulation of miR-3174 expression in cells.

Keywords

Cisatracurium Breast cancer miR-3174 Cell proliferation Migration Invasion.

Yang, X., & Ma, W. (2020). Cisatracurium inhibits the proliferation, migration and invasion of breast cancer cells by regulating the expression of miR-3174. Cellular and Molecular Biology, 66(6), 1–7. https://doi.org/10.14715/cmb/2020.66.6.1

Download Citation

References

Zhou Li. Advances in simplified MRI screening techniques for breast cancer [J]. J Clin Radiol 2018; 37(10):1766-1769.
Liuqin J, Le J, Yanbin L. Comparative study on the effects of rocuronium bromide and cisatracurium besylate on hemodynamics and stress response in patients undergoing radical mastectomy for breast cancer [J]. Chin Med Herald 2018; 15(34):108-111.
Wenwen H, Muhan L, Dongxin W. Impact of conventional anesthesia ways, drug selection and intraoperative management of tumor surgery on the prognosis of elderly patients [J]. J Clin Anesthesiol 2018; 34(7):94-98.
Yuhe ZH, Lili W, Zhitao W. Effect of sevoflurane on invasion and migration of breast cancer cells and its mechanism [J]. Int J Pathol Clin Med 2019; 39(2):10-16.
Yabasin IB, Sanches JG, Ibrahim MM, Huidan J, Williams W, Lu ZL, Wen Q. Cisatracurium retards cell migration and invasion upon upregulation of p53 and inhibits the aggressiveness of colorectal cancer. Front Physiol 2018; 9:941.
Taherdangkoo K, Kazemi NS, Hajjari MR, Tahmasebi BM. miR-485-3p suppresses colorectal cancer via targeting TPX2. Bratisl Lek Listy 2020; 121(4):302.
Sun W, Li W, Chen J, Teng Y, Ding X. MicroRNA-520b targets interleukin-2 to regulate the growth, metastasis and drug sensitivity of liver cancer cells. Am J Transl Res 2020; 12(4):1267.
Zhang DL, Yang N. MiR-3174 functions as an oncogene in rectal cancer by targeting PCBD2. Eur Rev Med Pharmacol Sci 2019; 23(6):2417-26.
Lv W, Wang J, Zhang S. Effects of cisatracurium on epithelial to mesenchymal transition in esophageal squamous cell carcinoma. Oncol Lett 2019;18(5):5325-31.
Chen B, Hu Z, Chen B, Li B. Effects and mechanism of Lanthanum Citrate on the proliferation and apoptosis of hepatocellular carcinoma cell line SMMC-7721. Turk J Gastroenterol 2020; 31(3):264.
Wang W, Li X, Ji D. Molecular mechanism of the effect of angiopoietin like protein 8 on the proliferation, invasion and migration of placental trophoblasts in preeclampsia. Exp Ther Med 2020; 19(6):3461-8.
Lu M, Huang L, Tang Y, Sun T, Li J, Xiao S, Zheng X, Christopher O, Mao H. ARNTL2 knockdown suppressed the invasion and migration of colon carcinoma: decreased SMOC2-EMT expression through inactivation of PI3K/AKT pathway. Am J Transl Res 2020;12(4):1293.
Li H, Zhao J, Jia X, Zhang Y, Du Y, Li H, Ma L, Huang JA. miR-21 promotes growth, invasion and migration of lung cancer cells by AKT/P-AKT/cleaved-caspase 3/MMP-2/MMP-9 signaling pathway. Int J Clin Exp Pathol 2020; 13(4):692.
Zhang H, Tulahong A, Wang W, Nuerrula Y, Zhang Y, Wu G, Mahati S, Zhu H. Downregulation of microRNA-519 enhances development of lung cancer by mediating the E2F2/PI3K/AKT axis. Int J Clin Exp Pathol 2020; 13(4):711.
Wang Q, Yang X, Zhou X, Wu B, Zhu D, Jia W, Chu J, Wang J, Wu J, Kong L. MiR-3174 promotes proliferation and inhibits apoptosis by targeting FOXO1 in hepatocellular carcinoma. Biochem Biophys Res Commun 2020.
Li B, Wang L, Li Z, Wang W, Zhi X, Huang X, Zhang Q, Chen Z, Zhang X, He Z, Xu J. miR-3174 contributes to apoptosis and autophagic cell death defects in gastric cancer cells by targeting ARHGAP10. Mol Ther Nucleic Acids 2017; 9:294-311.
Taheri Z, Asadzadeh Aghdaei H, Irani S, Modarressi MH, Noormohammadi Z. Clinical Correlation of miR-200c/141 Cluster DNA Methylation and miR-141 Expression with the Clinicopathological Features of Colorectal Primary Lesions/Tumors. Rep Biochem Mol Biol, 2019; 8(3):208-215.
Esfandi F, Fallah H, Arsang-Jang S, Taheri M, Ghafouri-Fard S. The Expression of CCAT2, UCA1, PANDA and GHET1 Long Non-coding RNAs in Lung Cancer. Rep Biochem Mol Biol, 2019; 8(1):36-41.
Azari I, Ghafouri-Fard S, Omrani MD, Arsang-Jang S, Kordi Tamandani DM, Saroone Rigi M, Rafiee S, Pouresmaeili F, Taheri M. Expression of Long Non-Coding RNAs in Placentas of Intrauterine Growth Restriction (IUGR) Pregnancies. Rep Biochem Mol Biol 2019; 8(1):25-31.
Akbarabadi A, Ismaili A, Kahrizi D, Firouzabadi FN. Validation of expression stability of reference genes in response to herbicide stress in wild oat (Avena ludoviciana). Cell Mol Biol 2018; 64(4). 113-118.
Ghorbani T, Kahrizi D, Saeidi M, Arji I. Effect of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression. Cell Mol Biol, 63(11): 32-36.
Kahrizi D, Ghari SM, Ghaheri M, Fallah F, Ghorbani T, Beheshti AA, Kazemi E, Ansarypour Z. Effect of KH2PO4 on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol, 63(7): 107-111.
Fallah F, Nokhasi F, Ghaheri M, Kahrizi D, Beheshti Ale AA, Ghorbani T, Kazemi E, Ansarypour Z. Effect of salinity on gene expression, morphological and biochemical characteristics of Stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol 2017; 63(7): 102-106.
Bordbar M, Darvishzadeh R, Pazhouhandeh M, Kahrizi D. An overview of genome editing methods based on endonucleases. Mod Genet J 2020; 15(2): 75-92.
Ghaheri M, Kahrizi D, Bahrami G, Mohammadi-Motlagh HR. Study of gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni under various mannitol concentrations. Mol Biol Rep 2019; 46(1): 7-16.
Eruygur N, Ucar E, Akpulat HA, Shahsavari K, Safavi SM, Kahrizi D. In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium. Mol Biol Rep 2019; 46(2): 2121-9.
Esmaeili F, Ghaheri M, Kahrizi D, Mansouri M, Safavi SM, Ghorbani T, Muhammadi S, Rahmanian E, Vaziri S. Effects of various glutamine concentrations on gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 1-5.
Akbari F, Arminian A, Kahrizi D, Fazeli A, Ghaheri M. Effect of nitrogen sources on gene expression of Stevia rebaudiana (Bertoni) under in vitro conditions. Cell Mol Biol, 64(2): 11-16.
Ghaheri M, Adibrad E, Safavi SM, Kahrizi D, Soroush A, Muhammadi S, Ghorbani T, Sabzevari A, Ansarypour Z, Rahmanian E. Effects of life cycle and leaves location on gene expression and glycoside biosynthesis pathway in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 17-22.
Kahrizi D, Ghaheri M, Yari Z, Yari K, Bahraminejad S. Investigation of different concentrations of MS media effects on gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 23-27.
Hashempoor S, Ghaheri M, Kahrizi D, Kazemi N, Muhammadi S, Safavi SM, Ghorbani T, Rahmanian E, Heshmatpanaah M. Effects of different concentrations of mannitol on gene expression in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 28-31.

References

Zhou Li. Advances in simplified MRI screening techniques for breast cancer [J]. J Clin Radiol 2018; 37(10):1766-1769.

Liuqin J, Le J, Yanbin L. Comparative study on the effects of rocuronium bromide and cisatracurium besylate on hemodynamics and stress response in patients undergoing radical mastectomy for breast cancer [J]. Chin Med Herald 2018; 15(34):108-111.

Wenwen H, Muhan L, Dongxin W. Impact of conventional anesthesia ways, drug selection and intraoperative management of tumor surgery on the prognosis of elderly patients [J]. J Clin Anesthesiol 2018; 34(7):94-98.

Yuhe ZH, Lili W, Zhitao W. Effect of sevoflurane on invasion and migration of breast cancer cells and its mechanism [J]. Int J Pathol Clin Med 2019; 39(2):10-16.

Yabasin IB, Sanches JG, Ibrahim MM, Huidan J, Williams W, Lu ZL, Wen Q. Cisatracurium retards cell migration and invasion upon upregulation of p53 and inhibits the aggressiveness of colorectal cancer. Front Physiol 2018; 9:941.

Taherdangkoo K, Kazemi NS, Hajjari MR, Tahmasebi BM. miR-485-3p suppresses colorectal cancer via targeting TPX2. Bratisl Lek Listy 2020; 121(4):302.

Sun W, Li W, Chen J, Teng Y, Ding X. MicroRNA-520b targets interleukin-2 to regulate the growth, metastasis and drug sensitivity of liver cancer cells. Am J Transl Res 2020; 12(4):1267.

Zhang DL, Yang N. MiR-3174 functions as an oncogene in rectal cancer by targeting PCBD2. Eur Rev Med Pharmacol Sci 2019; 23(6):2417-26.

Lv W, Wang J, Zhang S. Effects of cisatracurium on epithelial to mesenchymal transition in esophageal squamous cell carcinoma. Oncol Lett 2019;18(5):5325-31.

Chen B, Hu Z, Chen B, Li B. Effects and mechanism of Lanthanum Citrate on the proliferation and apoptosis of hepatocellular carcinoma cell line SMMC-7721. Turk J Gastroenterol 2020; 31(3):264.

Wang W, Li X, Ji D. Molecular mechanism of the effect of angiopoietin like protein 8 on the proliferation, invasion and migration of placental trophoblasts in preeclampsia. Exp Ther Med 2020; 19(6):3461-8.

Lu M, Huang L, Tang Y, Sun T, Li J, Xiao S, Zheng X, Christopher O, Mao H. ARNTL2 knockdown suppressed the invasion and migration of colon carcinoma: decreased SMOC2-EMT expression through inactivation of PI3K/AKT pathway. Am J Transl Res 2020;12(4):1293.

Li H, Zhao J, Jia X, Zhang Y, Du Y, Li H, Ma L, Huang JA. miR-21 promotes growth, invasion and migration of lung cancer cells by AKT/P-AKT/cleaved-caspase 3/MMP-2/MMP-9 signaling pathway. Int J Clin Exp Pathol 2020; 13(4):692.

Zhang H, Tulahong A, Wang W, Nuerrula Y, Zhang Y, Wu G, Mahati S, Zhu H. Downregulation of microRNA-519 enhances development of lung cancer by mediating the E2F2/PI3K/AKT axis. Int J Clin Exp Pathol 2020; 13(4):711.

Wang Q, Yang X, Zhou X, Wu B, Zhu D, Jia W, Chu J, Wang J, Wu J, Kong L. MiR-3174 promotes proliferation and inhibits apoptosis by targeting FOXO1 in hepatocellular carcinoma. Biochem Biophys Res Commun 2020.

Li B, Wang L, Li Z, Wang W, Zhi X, Huang X, Zhang Q, Chen Z, Zhang X, He Z, Xu J. miR-3174 contributes to apoptosis and autophagic cell death defects in gastric cancer cells by targeting ARHGAP10. Mol Ther Nucleic Acids 2017; 9:294-311.

Taheri Z, Asadzadeh Aghdaei H, Irani S, Modarressi MH, Noormohammadi Z. Clinical Correlation of miR-200c/141 Cluster DNA Methylation and miR-141 Expression with the Clinicopathological Features of Colorectal Primary Lesions/Tumors. Rep Biochem Mol Biol, 2019; 8(3):208-215.

Esfandi F, Fallah H, Arsang-Jang S, Taheri M, Ghafouri-Fard S. The Expression of CCAT2, UCA1, PANDA and GHET1 Long Non-coding RNAs in Lung Cancer. Rep Biochem Mol Biol, 2019; 8(1):36-41.

Azari I, Ghafouri-Fard S, Omrani MD, Arsang-Jang S, Kordi Tamandani DM, Saroone Rigi M, Rafiee S, Pouresmaeili F, Taheri M. Expression of Long Non-Coding RNAs in Placentas of Intrauterine Growth Restriction (IUGR) Pregnancies. Rep Biochem Mol Biol 2019; 8(1):25-31.

Akbarabadi A, Ismaili A, Kahrizi D, Firouzabadi FN. Validation of expression stability of reference genes in response to herbicide stress in wild oat (Avena ludoviciana). Cell Mol Biol 2018; 64(4). 113-118.

Ghorbani T, Kahrizi D, Saeidi M, Arji I. Effect of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression. Cell Mol Biol, 63(11): 32-36.

Kahrizi D, Ghari SM, Ghaheri M, Fallah F, Ghorbani T, Beheshti AA, Kazemi E, Ansarypour Z. Effect of KH2PO4 on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol, 63(7): 107-111.

Fallah F, Nokhasi F, Ghaheri M, Kahrizi D, Beheshti Ale AA, Ghorbani T, Kazemi E, Ansarypour Z. Effect of salinity on gene expression, morphological and biochemical characteristics of Stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol 2017; 63(7): 102-106.

Bordbar M, Darvishzadeh R, Pazhouhandeh M, Kahrizi D. An overview of genome editing methods based on endonucleases. Mod Genet J 2020; 15(2): 75-92.

Ghaheri M, Kahrizi D, Bahrami G, Mohammadi-Motlagh HR. Study of gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni under various mannitol concentrations. Mol Biol Rep 2019; 46(1): 7-16.

Eruygur N, Ucar E, Akpulat HA, Shahsavari K, Safavi SM, Kahrizi D. In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium. Mol Biol Rep 2019; 46(2): 2121-9.

Esmaeili F, Ghaheri M, Kahrizi D, Mansouri M, Safavi SM, Ghorbani T, Muhammadi S, Rahmanian E, Vaziri S. Effects of various glutamine concentrations on gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 1-5.

Akbari F, Arminian A, Kahrizi D, Fazeli A, Ghaheri M. Effect of nitrogen sources on gene expression of Stevia rebaudiana (Bertoni) under in vitro conditions. Cell Mol Biol, 64(2): 11-16.

Ghaheri M, Adibrad E, Safavi SM, Kahrizi D, Soroush A, Muhammadi S, Ghorbani T, Sabzevari A, Ansarypour Z, Rahmanian E. Effects of life cycle and leaves location on gene expression and glycoside biosynthesis pathway in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 17-22.

Kahrizi D, Ghaheri M, Yari Z, Yari K, Bahraminejad S. Investigation of different concentrations of MS media effects on gene expression and steviol glycosides accumulation in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 23-27.

Hashempoor S, Ghaheri M, Kahrizi D, Kazemi N, Muhammadi S, Safavi SM, Ghorbani T, Rahmanian E, Heshmatpanaah M. Effects of different concentrations of mannitol on gene expression in Stevia rebaudiana Bertoni. Cell Mol Biol, 64(2): 28-31.

Author biographies is not available.