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High-performance thin-layer chromatography fingerprinting, total phenolic and total flavonoid contents and anti-platelet-aggregation activities of Prosopis farcta extracts
Corresponding Author(s) : Javad Sharifi-Rad
javad.sharifirad@gmail.com
Cellular and Molecular Biology,
Vol. 66 No. 4: Medicinal plants and natural products
Abstract
Cardiovascular diseases are a leading cause of worldwide death and excessive platelet is closely related with their pathogenesis. Different plants and natural compounds have demonstrated anti-platelet effects. The aim of this study was to report the high-performance thin-layer chromatography (HPTLC) fingerprinting and anti-platelet-aggregation activities of different leaf extracts (n-hexane, chloroform, ethyl acetate, methanol and aqueous) of Prosopis farcta (Syrian mesquite) plant. The results showed a 100% inhibition of aggregation activity after plasmatic adenosine diphosphate (ADP) aggregation activation of ethyl acetate, ethanolic, methanolic and aqueous extracts, at 60 mg/mL concentration. The IC50 ADP value of these extracts ranged between 4.07 and 11.39 mg/mL. Moreover, these extracts reported the highest amounts of phenolic and flavonoid contents. In conclusion, phytochemicals present in P. farcta leaves have anti-platelet-aggregation activities. Future studies are needed to identify the compounds with anti-platelet potential present in P. farcta.
Keywords
Syrian mesquite
Antiplatelet
Cardiovascular disease
Phytochemical
HPTLC.
Kobarfard, F., Ayatollahi, S. A., Khosravi-Dehaghi, N., Faizi, M., Amidi, S., Martorell, M., Choudhary, M. I., Suleria, H. A. R., & Sharifi-Rad, J. (2020). High-performance thin-layer chromatography fingerprinting, total phenolic and total flavonoid contents and anti-platelet-aggregation activities of Prosopis farcta extracts. Cellular and Molecular Biology, 66(4), 8–14. https://doi.org/10.14715/cmb/2020.66.4.2
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