Issue
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
Molecular and characterization of NnPPO cDNA from lotus (Nelumbo nucifera) in rhizome browning
Corresponding Author(s) : C Dong
Cellular and Molecular Biology,
Vol. 62 No. 4: Issue 4
Abstract
The complete cDNA (NnPPO) of polyphenol oxidase in Nelumbo nucifera was successfully isolated, using Rapid amplification cDNA end (RACE) assays. The full-length cDNA of NnPPO was 2069 bp in size, containing a 1791 bp open reading frame coding 597 amino acids. The putative NnPPO possessed the conserved active sites and domains for PPO function. Phylogenetic analysis revealed that NnPPO shared high homology with PPO of high plants, and the homology modeling proved that NnPPO had the typical structure of PPO family. In order to characterize the role of NnPPO, Real-time PCR assay demonstrated that NnPPO mRNA was expressed in different tissues of N. nucifera including young leave, rhizome, flower, root and leafstalk, with the highest expression in rhizome. Patterns of NnPPO expression in rhizome illustrated its mRNA level was significantly elevated, which was consistent with the change of NnPPO activity during rhizome browning. Therefore, transcriptional activation of NnPPO was probably the main reason causing rhizome browning.
Keywords
Nelumbo nucifera
Polyphenol oxidase
cDNA
Tissue expression
Rhizome browning.
Dong, C., Yu, A. Q., Yang, M. G., Zhou, M. Q., & Hu, Z. L. (2016). Molecular and characterization of NnPPO cDNA from lotus (Nelumbo nucifera) in rhizome browning. Cellular and Molecular Biology, 62(4), 67–72. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/843
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX