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Vol. 65 No. 7: Issue 7

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WT1 influences apoptosis and proliferation of immature mice granular cells through regulation of the wnt/β-catenin signal pathway

https://doi.org/10.14715/cmb/2019.65.7.24
Juan Wang
Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou, China
Jianping Qiu
The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China
Le Bo
Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou, China
Zhinan Wu
Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou, China
Anwen Zhou
Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou, China
Wendan Xu
Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou, China
Caiping Mao
Reproductive Medicine Center, The First Affiliated Hospital of Soochow University, Suzhou, China

Corresponding Author(s) : Caiping Mao

trnm07@163.com

Cellular and Molecular Biology, Vol. 65 No. 7: Issue 7

Abstract

It was to study the influence of Wilms tumor suppressor gene (WT1) on ovarian granular cells (GCs) in mice, and the molecular mechanism involved. LV-WT1 short hairpin ribonucleic acid (shRNA) vector was used to downregulate WT1 expression in granular cells (GCs). The effects of WTI on proliferation and apoptosis of GCs were investigated. Western blot and qRT-PCR were used to assay the mRNA and protein expressions of Bax/bcl-2 in GCs transfected with LV-WT1-RNAi. The expression levels of SUZ12, Wnt5a, Wnt11, Wnt4, Wnt3a, Wnt2 mRNA in GCs were also determined. LV-WT1-RNAi significantly reduced WT1 expression, increased apoptosis and inhibited proliferation of GCs. The inhibition of WT1 had no significant effect on the expression of bcl-2 in GCs. The expressions of Wnt2, Wnt4 and Wnt5a were augmented in WT1-knockdown GCs, relative to non-transfected cells. WT1 activation is necessary for maintaining early survival of GCs in follicles via activation of the Wnt/β-catenin signal pathway.

Keywords

WT1 Apoptosis Granulosa cells Wnt/β-catenin signaling.
Wang, J., Qiu, J., Bo, L., Wu, Z., Zhou, A., Xu, W., & Mao, C. (2019). WT1 influences apoptosis and proliferation of immature mice granular cells through regulation of the wnt/β-catenin signal pathway. Cellular and Molecular Biology, 65(7), 138–145. https://doi.org/10.14715/cmb/2019.65.7.24
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