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Vol. 66 No. 2: Issue 2

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Effect of glutamine on the immune function of paclitaxel intervention in ovarian cancer mice

https://doi.org/10.14715/cmb/2020.66.2.30
Li Wang
Department of Gynecology, Weifang People's Hospital, Weifang 261000, China
Yanfen Li
Department of Oncology, Weifang People's Hospital, Weifang 261000, China
Juan Wang
Department of Clinical Nutrition, Weifang People's Hospital 261000, China

Corresponding Author(s) : Juan Wang

wwb0718@126.com

Cellular and Molecular Biology, Vol. 66 No. 2: Issue 2

Abstract

To analyze the effect of amide Valley on the immune function of ovarian cancer mice treated with paclitaxel. Fifty SPF female BALB/c mice were selected as experimental subjects, and the mice were divided into five groups, namely normal control group, tumor control group, paclitaxel group, glutamine group and combined intervention group, with 10 mice in each group. Except for the normal control group, the other 40 mice were all subjected to ovarian cancer modeling. After modeling, the tumor control group mice were injected with saline at a dose of 25 mg/kg; the mice in the paclitaxel group were given a dose of 25 mg/kg. Intraperitoneal injection of paclitaxel; mice in the glutamine group were intraperitoneally injected with glutamine at a dose of 25 mg/kg; mice in the joint intervention group were intraperitoneally injected with glutamine and paclitaxel at a dose of 25 mg/kg once daily. The normal control mice did not receive any treatment. Observe and compare the status of the five groups of mice and regularly measure the quality of the mice; after 2 weeks of treatment, the CD3+, CD4, CD4+, CD8, CD8+ of the T lymphocyte subsets of each group of mice were detected by flow cytometry calculate the ratio of CD4 / CD8. The tumor formation rate of mouse ovarian cancer was 95% (38/40). The hair color, diet, excretion and activity of mice in the normal control group were normal, while the hair of the remaining four groups of ovarian cancer mice was sparse and dull compared with those in the normal control group, and the diet was decreased and the action was retarded. By measuring the body mass of mice at a regular time, we found that before treatment, the body mass of the other four groups of ovarian cancer mice increased significantly (P< 0.05) because of the tumor body compared with the normal control group mice; after treatment, compared with the other ovarian cancer mice group, the body mass of the combined intervention group mice decreased significantly, the difference was statistically significant (P< 0.05), suggesting that the tumor body was reduced. Compare with normal control mice, CD3+, CD4, and CD4 / CD8 of other ovarian cancer mice groups were significantly decreased, and CD8+ was significantly increased, the differences were statistically significant (P< 0.05); There was no significant difference in the levels of CD3+, CD4+, CD4 / CD8, and CD8+ between the tumor control group and the paclitaxel group (P> 0.05). Paclitaxel does not improve the immunity of patients during the treatment of ovarian cancer. Glutamine is effective immunomodulation. By regulating paclitaxel-treated ovarian cancer mice, it can simultaneously treat ovarian cancer, significantly improve the immune function of ovarian cancer mice, thereby improving the anti-tumor ability, and provide the possibility of significantly improving the body's immunity of ovarian cancer patients. Clinical research and long-term prognosis still need to be confirmed by further studies.

Keywords

Glutamine Paclitaxel Ovarian cancer Immune function
Wang, L., Li, Y., & Wang, J. (2020). Effect of glutamine on the immune function of paclitaxel intervention in ovarian cancer mice. Cellular and Molecular Biology, 66(2), 193–197. https://doi.org/10.14715/cmb/2020.66.2.30
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References
  1. Zefei J, Santai S. New progress in clinical application of paclitaxel in breast cancer chemotherapy. Chin J Cancer 1997; 4: 289-292.
  2. Razzouk S. Translational genomics and head and neck cancer: toward precision medicine. Clin Genet 2014; 86(5): 412-421.
  3. Li W, Jia MX, Wang JH, Lu JL, Deng J, Tang JX and Liu C: Association of MMP9-1562C/T and MMP13-77A/G polymorphisms with non-small cell lung cancer in southern Chinese population. Biomol 9(3): 107-119, 2019.
  4. Liang Y, Lin Q, Huang P, Wang Y, Li J, Zhang L and Cao J: Rice Bioactive Peptide Binding with TLR4 To Overcome H2O2-Induced Injury in Human Umbilical Vein Endothelial Cells through NF-κB Signaling. J Agri Food Chem 2018; 66(2): 440-448.
  5. Wang L, Lin Q, Yang T, Liang Y, Nie Y, Luo Y and Luo F: Oryzanol modifies high fat diet-induced obesity, liver gene expression profile, and inflammation response in mice. J Agri Food Chem 2017; 65(38): 8374-8385.
  6. Lou Y, Shi J, Guo D, Qureshi AK and Song L: Function of PD-L1 in antitumor immunity of glioma cells. Saudi J Boil Sci 2017; 24(4): 803-807.
  7. Guo T, Lin Q, Li X, Nie Y, Wang L, Shi L and Luo F: Octacosanol attenuates inflammation in both RAW264. 7 macrophages and a mouse model of colitis. J Agri Food Chem 2017; 65(18): 3647-3658.
  8. Li W, Jia MX, Wang JH, Lu JL, Deng J, Tang JX and Liu C: Association of MMP9-1562C/T and MMP13-77A/G polymorphisms with non-small cell lung cancer in southern Chinese population. Biomol 2019; 9(3): 107-119.
  9. Nie Y, Luo F, Wang L, Yang T, Shi L, Li X, Shen J, Xu W, Guo T and Lin Q: Anti-hyperlipidemic effect of rice bran polysaccharide and its potential mechanism in high-fat diet mice. Food Func 2017; 8(11): 4028-4041.
  10. Lou Y, Yang J, Wang L, Chen X, Xin X and Liu Y: The clinical efficacy study of treatment to Chiari malformation type I with syringomyelia under the minimally invasive surgery of resection of Submeningeal cerebellar Tonsillar Herniation and reconstruction of Cisterna magna. Saudi J Biol Sci 2019; 26(8): 1927-1931.
  11. Lou Y, Guo D, Zhang H and Song L: Effectiveness of mesenchymal stems cells cultured by hanging drop vs. conventional culturing on the repair of hypoxic-ischemic-damaged mouse brains, measured by stemness gene expression. Open Life Sci 2016; 11(1): 519-523.
  12. Chen X, Xu Y, Meng L, Chen X, Yuan L, Cai Q, Shi W and Huang G: Non-parametric partial least squares–discriminant analysis model based on sum of ranking difference algorithm for tea grade identification using electronic tongue data identify tea grade using e-tongue data. Sens Actuators B Chem 2020; 127924.
  13. Nie Y, Luo F and Lin Q: Dietary nutrition and gut microflora: A promising target for treating diseases. Trends Food Sci Technol 2018;75: 72-80.
  14. Ren Y, Jiao X and Zhang L: Expression level of fibroblast growth factor 5 (FGF5) in the peripheral blood of primary hypertension and its clinical significance. Saudi J Biol Sci 2018; 25(3): 469-473.
  15. Yang X, Hui P, Xuelian S. Study on the mechanism of down-regulated expression promoting senescence of ovarian cancer cells. Chin J Pathophys 2019; 35(9): 1565-1572.
  16. Junkai X, Qisong C, Yunhui Z. Clinical observation of paclitaxel and Nedaplatin Combined with radiotherapy for esophageal cancer. Chin Med Guide 2019; (24).
  17. Ying L, Ji Linhua review, Cui Sen proofreading. Research progress on new glutaminase inhibitors and their antitumor activity. Chin Cancer Clinic 2019; (7): 366-369.
  18. Liansheng P, Wanchun Z. Application value of peripheral blood lymphocyte immunophenotype test in evaluation of cellular immune function in patients with malignant tumors. Int J Immuno 2019; 42(3): 280-284.
  19. DeBerardinis RJ, Cheng T. Q's next: the diverse functions of glutamine in metabolism, cell biology and cancer. Oncogene 2010; 29(3):313-24.
  20. Mingqing G, Limei M. Application of Enteral Nutrition Enhanced by Astragalus membranaceus and Glutamine in Severe Craniocerebral Injury. Chine Folk Therapy 2019; (12).
  21. Yue S. Study on selective toxicity of ibuprofen to zebrafish adults based on omics technology. 2018.
  22. Richard V, Dahiya D, Kaman L, Raj P, Behera A. Effect of perioperative glutamine administration on C-reactive protein and liver function tests in patients undergoing hepatic resection. Pol J Surg 2014 Jan 1;86(1):11-6.
  23. Wei C, Qiping L, Shenghui L, et al. Effects of glutamine immunonutrition on liver function, lymphocyte count and C-reactive protein in patients undergoing hepatectomy at nutritional risk. Chinese Journal of Experimental Surgery, 2017, 34 (06): 1027-1030.
  24. Brasse-Lagnel C, Lavoinne A, Husson A. (2009). Control of mammalian gene expression by amino acids, especially glutamine. FEBS J 276: 1826–1844.
  25. Xiaohe C, Wenge M, Yongcun L, Xiaofei L, Jinfeng W, Shuanzhu Z, Peng S, Dawei Y. Effect of Alanyl Glutamine Glutamine on Increasing Immunological Function in Surgical Critical Patients% Alanyl-Glutamine on Improving Immune Function in Surgical Critical Patients. China Pharm Indu 2013; 022 (010): 53-54.
  26. Xiaoguang F, Jin P, Guanghu Y. Effect of alanyl glutamine on coagulation function and cytokines in sepsis patients. Chin J Hospital Infect 2019; 17: 2584-2588.
  27. Hua Y, Chen Guoqing. The role of immunonutrients in nutritional support therapy. Chinese J Digest Surgery 2019; 18(10): 912-915.
  28. Aiken KJ, Bickford JS, Kilberg MS, Nick HS . (2008). Metabolic regulation of manganese superoxide dismutase expression via essential amino acid deprivation. J Biol Chem 283: 10252–10263.
  29. DeBerardinis RJ, Sayed N, Ditsworth D, Thompson CB. Brick by brick: metabolism and tumor cell growth. Curr Opin Genet Dev 2008; 18: 54–61.
  30. Ahmadi AR, Rayyani E, Bahreini M, Mansoori A. The Effect of Glutamine Supplementation on Athletic Performance, Body Composition, and Immune Function: A Systematic Review and a Meta-Analysis of Clinical Trials. Clinic Nut 2018; 38(3): 1076-1091.
  31. Estrela JM, Ortega A, Obrador E. Glutathione in cancer biology and therapy. Crit Rev Clin Lab Sci 2006; 43: 143–181.
  32. Ma C, Tsai H, Su W, Sun L, Shih Y, Wang J. Combination of arginine, glutamine, and omega-3 fatty acid supplements for perioperative enteral nutrition in surgical patients with gastric adenocarcinoma or gastrointestinal stromal tumor (GIST): A prospective, randomized, double-blind study. J postgraduate Med 2018; 64(3):155-163.
  33. Haskins SC. Nutritional support of critical patients[m]// manual of small animal emergency and critical care medicine, second edition. John Wiley & Sons Ltd, 2012; 116-136.
  34. PaíŸlack N, Doherr MG, Zentek J. Effects of free amino acids on cytokine secretion and proliferative activity of feline T cells in an in vitro study using the cell line MYA-1. Cytotechnology 2016; 68(5):1949-1961.
  35. Chitapanarux I. Randomized study of nutritional status and treatment toxicities of oral arginine, glutamine, and Omega-3 fatty acids during concurrent chemoradiotherapy for head and neck cancer patients. Func Food Health Disease 2016; 6(3): 121-132.
  36. Lo M, Wang YZ, Gout PW. The x(c)- cystine/glutamate antiporter: a potential target for therapy of cancer and other diseases. J Cell Physiol 2008; 215: 593–602.
  37. Kroemer G, Pouyssegur J. Tumor cell metabolism: cancer's Achilles' heel. Cancer Cell 2008; 13: 472–482.
  38. Estrela JM, Ortega A, Obrador E. Glutathione in cancer biology and therapy. Crit Rev Clin Lab Sci 2006; 43: 143–181.
  39. DeBerardinis RJ. Is cancer a disease of abnormal cellular metabolism? New angles on an old idea. Genet Med 2008; 10: 767–777.
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