Cell dedifferentiation and multiplication of Burdock (Arctium Lappa) as a medicinal plant

Alireza Zebarjadi; Samaneh Kazem; Danial Kahrizi

doi:10.14715/cmb/2018.64.7.16

Issue

Vol. 64 No. 7: Issue 7

Issue Published : June 12, 2018

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Cell dedifferentiation and multiplication of Burdock (Arctium Lappa) as a medicinal plant

https://doi.org/10.14715/cmb/2018.64.7.16

Alireza Zebarjadi

Department of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah, Iran

Samaneh Kazem

Department of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah, Iran

Danial Kahrizi

Department of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah, Iran

Corresponding Author(s) : Alireza Zebarjadi

zebarjadiali@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 7: Issue 7
Article Published : May 30, 2018

Abstract

Arctium lappa L. (Burdock) is an important plant with various pharmacological effects. According to the importance of this plant, optimization of its tissue culture will lead to more investigation and application of it. The aim of this study was to develop protocols for callus induction and shoot regeneration of A. lappa. In order to optimize of tissue culture in A. lappa, callus induction, indirect regeneration and direct regeneration were carried out in factorial experiment based on Completely Randomized Designs (CRDs). Hypocotyl and cotyledon were cultured on the Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BAP) for callus induction. In indirect regeneration experiment various levels of BAP and Î±-Naphthaleneacetic acid (NAA) and two types of explants (calli derived from cotyledon and hypocotyl) were investigated. In direct regeneration section, various levels of BAP plus 2 mg/l NAA and different explants (cotyledon, hypocotyl and bud) were compared. In both cotyledon and hypocotyl, the maximum callus induction was observed on a media containing 2 mg/l 2,4-D plus 1 mg/l BAP (100% and 76.19% respectively). The highest percentage of indirect regeneration (65%) was observed at 1 mg/l BAP plus 0.5 mg/l NAA on calli from hypocotyl. The highest percentage of direct regeneration (90.33) was observed in hypocotyl with a lateral bud explant on MS medium supplemented with 0.5 mg/l BAP plus 2 mg/l NAA. In this study, optimization of tissue culture protocol for A. lappa was carried out as a research technique, as well as technique for further exploitation of this plant.

Keywords

Cell dedifferentiation Callus induction Plant regeneration Burdock Micropropagation.

Zebarjadi, A., Kazem, S., & Kahrizi, D. (2018). Cell dedifferentiation and multiplication of Burdock (Arctium Lappa) as a medicinal plant. Cellular and Molecular Biology, 64(7), 92–96. https://doi.org/10.14715/cmb/2018.64.7.16

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References

Gross S, Werner A, Hawthorn R: The Biology of Canadian Weeds. 38. Arctium Minus (Hill) Bernh. and A. Lappa L. Can J Plant Sci 60: 621-634, 1980.
Li D, Kim JM, Jin Z, Zhou J; Prebiotic effectiveness of inulin extracted from edible burdock. Food Sci Technol. 14(1):29-34, 2008.
Chang HJ, Huang WT, Tsao DA, Huang KM, Lee SC, Lin SR, Yang SC, Yeh CS: Identification and Authentication of Burdock (Arctium lappa Linn) Using PCR Sequencing. Fooyin J Health Sci 1:28âˆ’32, 2009.
Kardosova A, Ebringerova A, Alfoldi J, Nosolova G, Franova S, Hribalova V: A biologically active fructan from the roots of Arctium lappa L., var. Herkules. Int J Biol Macromolec 33:135–140, 2003.
Duh P: Antioxidant Activity of Burdock (Arctium lappa Linné): Its Scavenging Effect on Free-Radical and Active Oxygen. J Am Oil Chem Soc 75:455-461, 1998.
Vianna J, Baldoqui DC, Odair J, Castro S, Rodrigues RCL, Yara T: Antimicrobial Activity of Arctium lappa Constituents Against Microorganisms Commonly Found in Endodontic Infections. Braz Dent J 16:192-196, 2005.
Knipping K, Esch E, Wijering SC, Heide S, Dubois AE, Garssen J: In Vitro and In Vivo Anti-Allergic Effects of Arctium lappa L. Exp Biol Med 233:1469-1477, 2008.
Taher MA, Abdul- Hussain DA, Hasan HF, Fahmi ZM, Luaibi OK, Ali MG: Hypolipidemic Effect of Caffeic Acid Isolated From Arctium Lappa Cultivated In Iraq, in Hyperlipidemic Rat Model. Iraqi J Pharm Sci. 24(1):18-24, 2015.
Lin SC, Chung TC, Lin CC, Ueng TH, Lin YH, Lin SY, Wang LY: Hepatoprotective effects of Arctium Lappa on carbon tetrachloride- and acetaminophen-induced liver damage. Am J Chin Med 28(2):163-173, 2000.
Lin SC, Lin CH, Lin CC, Lin YH, Chen CF, Chen IC. Wang LY: Hepatoprotective effects of Arctium lappa Linne on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride. J Biomed Sci. 9:401–409, 2002.
Mitsuo M, Nobuo Y, Katsuya T: Inhibitory compounds of Î±-glucosidase activity from Arctium lappa L. J Oleo Sci 54:589-594, 2005.
Ming DSH, Guns E, Eberding A, Towers GH: Isolation and Characterization of Compounds with Anti-prostate Cancer Activity from Arctium lappa L. Using Bioactivity-guided Fractionation. Pharm Biol 42:44-48, 2004.
Bhojwani SS, Dantu PK: Plant Tissue Culture: An Introductory Text. New Delhi, India: Springer, 2013
Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473- 497, 1962.
Duncan DB: Multiple range and multiple F tests. Biom. 11:1-42, 1955.
He WT, Hou SW, Wang SW: Callus induction and high-frequency plant regeneration from hypocotyl and cotyledon explants of Arctium lapp. In Vitro Cell Dev Biol, Plant 42:411–414, 2006.

References

Gross S, Werner A, Hawthorn R: The Biology of Canadian Weeds. 38. Arctium Minus (Hill) Bernh. and A. Lappa L. Can J Plant Sci 60: 621-634, 1980.

Li D, Kim JM, Jin Z, Zhou J; Prebiotic effectiveness of inulin extracted from edible burdock. Food Sci Technol. 14(1):29-34, 2008.

Chang HJ, Huang WT, Tsao DA, Huang KM, Lee SC, Lin SR, Yang SC, Yeh CS: Identification and Authentication of Burdock (Arctium lappa Linn) Using PCR Sequencing. Fooyin J Health Sci 1:28âˆ’32, 2009.

Kardosova A, Ebringerova A, Alfoldi J, Nosolova G, Franova S, Hribalova V: A biologically active fructan from the roots of Arctium lappa L., var. Herkules. Int J Biol Macromolec 33:135–140, 2003.

Duh P: Antioxidant Activity of Burdock (Arctium lappa Linné): Its Scavenging Effect on Free-Radical and Active Oxygen. J Am Oil Chem Soc 75:455-461, 1998.

Vianna J, Baldoqui DC, Odair J, Castro S, Rodrigues RCL, Yara T: Antimicrobial Activity of Arctium lappa Constituents Against Microorganisms Commonly Found in Endodontic Infections. Braz Dent J 16:192-196, 2005.

Knipping K, Esch E, Wijering SC, Heide S, Dubois AE, Garssen J: In Vitro and In Vivo Anti-Allergic Effects of Arctium lappa L. Exp Biol Med 233:1469-1477, 2008.

Taher MA, Abdul- Hussain DA, Hasan HF, Fahmi ZM, Luaibi OK, Ali MG: Hypolipidemic Effect of Caffeic Acid Isolated From Arctium Lappa Cultivated In Iraq, in Hyperlipidemic Rat Model. Iraqi J Pharm Sci. 24(1):18-24, 2015.

Lin SC, Chung TC, Lin CC, Ueng TH, Lin YH, Lin SY, Wang LY: Hepatoprotective effects of Arctium Lappa on carbon tetrachloride- and acetaminophen-induced liver damage. Am J Chin Med 28(2):163-173, 2000.

Lin SC, Lin CH, Lin CC, Lin YH, Chen CF, Chen IC. Wang LY: Hepatoprotective effects of Arctium lappa Linne on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride. J Biomed Sci. 9:401–409, 2002.

Mitsuo M, Nobuo Y, Katsuya T: Inhibitory compounds of Î±-glucosidase activity from Arctium lappa L. J Oleo Sci 54:589-594, 2005.

Ming DSH, Guns E, Eberding A, Towers GH: Isolation and Characterization of Compounds with Anti-prostate Cancer Activity from Arctium lappa L. Using Bioactivity-guided Fractionation. Pharm Biol 42:44-48, 2004.

Bhojwani SS, Dantu PK: Plant Tissue Culture: An Introductory Text. New Delhi, India: Springer, 2013

Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473- 497, 1962.

Duncan DB: Multiple range and multiple F tests. Biom. 11:1-42, 1955.

He WT, Hou SW, Wang SW: Callus induction and high-frequency plant regeneration from hypocotyl and cotyledon explants of Arctium lapp. In Vitro Cell Dev Biol, Plant 42:411–414, 2006.

Author biographies is not available.