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Molecular typing oF Escherichia coli strains isolated from food in casablanca (Morocco)
Corresponding Author(s) : S Badri
samira_bdm@yahoo.fr
Cellular and Molecular Biology,
Vol. 55 No. 4: General Papers
Abstract
Serotyping of O- and H-antigens is regarded as the gold standard in classification of E. coli for taxonomic and epidemiological purposes similar to the Kaufmann-White scheme for Salmonella enterica. Molecular methods to replace or to support the serotyping were recently applied. Using the molecular polymorphism of the somatic (O-antigen) gene rfb cluster and flagella (H-antigen) gene fliC, 74 E. coli strains carrying the virulent genes isolated from food were characterised by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that 49 (66%) of the isolates revealed a reproducible and clear cut classification, with a very good correlation to the collection of reference strains, were found.
Keywords
E. coli
Molecular typing
PCR-RFLP
Food
Morocco.
Badri, S., Fassouane, A., Filliol, I., Hassar, M., & Cohen, N. (2009). Molecular typing oF Escherichia coli strains isolated from food in casablanca (Morocco). Cellular and Molecular Biology, 55(4), 1132–37. Retrieved from https://mail.cellmolbiol.org/index.php/CMB/article/view/1055
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