Issue

Vol. 63 No. 10: Issue 10

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Cardioprotective effect of Pycnogenol in ischemic-reperfusion injury (IRI) in rats

https://doi.org/10.14715/cmb/2017.63.10.7
P. Xiao
Department of cardiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China
K. Zhang
Department of cardiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China
Z. Tao
Department of cardiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China
N. Liu
Department of cardiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China
B. Ge
Central laboratory, Nanjing first hospital, Nanjing Medical University, Nanjing 211100, China

Corresponding Author(s) : P. Xiao

xiaopingxi@hotmail.com

Cellular and Molecular Biology, Vol. 63 No. 10: Issue 10

Abstract

Oxidative stress plays a critical task in the biochemical and pathological alteration linked with myocardial ischemic-reperfusion injury (IRI). This warrants identifying agents with a potential for preventing such damage in an effective way. A novel plant based product, Pycnogenol, obtained from the French maritime pine (Pinus pinaster ssp. atlantica) bark extract was known for its tremendous antioxidant potential (both in vivo, in vitro). It was able to attenuate the symptoms of immune dysfunction through restoring a cellular antioxidant status in low micronutrient-induced immune deficient mice. Consequently, the present study was deals with the determination of protective effect of Pycnogenol in ischemic–reperfusion injury (IRI) in rats via Non-recirculating Langendorff's technique. The effect of Pycnogenol  on the level of various key biomarkers in the rat heart homogenate was determined, such as, myocardial thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation), lactic dehydrogenase (LDH) (a marker of tissue injury) and effect on endogenous antioxidants, e.g., superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione peroxidase (GPx). The activity of these biomarkers appreciably improved in Pycnogenol-treated group than IRI group (P < 0.05). The effect of Pycnogenol was further confirmed via histopathological examination of cardiac tissues, which suggests that, it considerably improved the injury related to tissue damage through suppression of edema and infiltration of neutrophil compared to IRI group. It also showed modulation of the expression of apoptotic factors, e.g. Bcl-2, bax and caspase-9 as confirmed by western blot analysis.

Keywords

Cardio vascular diseases Pycnogenol Ischemic–reperfusion injury.
Xiao, P., Zhang, K., Tao, Z., Liu, N., & Ge, B. (2017). Cardioprotective effect of Pycnogenol in ischemic-reperfusion injury (IRI) in rats. Cellular and Molecular Biology, 63(10), 49–53. https://doi.org/10.14715/cmb/2017.63.10.7
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. References
  2. Deaton, C., Froelicher, E.S., Wu, L.H., Ho, C., Shishani, K. and Jaarsma, T. The global burden of cardiovascular disease. Eur J Cardiovasc Nurs. 2011; 2: S5-13.
  3. Thomas, A.G. Reducing The Growing Burden Of Cardiovascular Disease In The Developing World. Health Aff (Millwood). 2007; 26: 13–24.
  4. Zucchi, R., Ghelardoni, S. and Evangelista, S. Biochemical basis of ischemic heart injury and of cardioprotective interventions. Curr Med Chem. 2007; 14: 1619–1637.
  5. Jain, A.K., Mehra, N.K. and Swarnakar, N.K. Role of antioxidants for the treatment of cardiovascular diseases: Challenges and opportunities. Curr Pharm Des. 2015; 21: 4441-4455.
  6. Victor, V.M. and Rocha, M. Targeting antioxidants to mitochondria: a potential new therapeutic strategy for cardiovascular diseases. Curr Pharm Des. 2007; 13: 845-863.
  7. Halliwell, B., Gutteridge, J.M. and Cross, C.E. Free radicals, antioxidants, and human disease: where are we now?. J Lab Clin Med. 1992; 119: 598–620.
  8. Buja, L.M. and Entman, M.L. Modes of myocardial cell injury and cell death in ischemic heart disease. Circulation. 1998; 98:1355–1357.
  9. Hearse, D.J. Prospects for antioxidant therapy in cardiovascular medicine. Am J Med. 1991; 91:118S–121S.
  10. Watson, R. Pycnogenol and cardiovascular health. Evidence-based integrative medicine. 2003; 1: 27-32.
  11. Watson, R.R. Reduction of cardiovascular disease risk factors by French maritime Pine bark extract. Cardiovascular Rev. Rep. 1999; 20: 326-329.
  12. Packer, L., Rimbach, G. and Virgili, F. Antioxidant activity and Biologic properties of a Procyanidin-rich extract from pine (Pinus Maritima) bark, Pychogenol. Free Radic Biol Med. 1999; 27: 704-724.
  13. Okhawa, H., Oohishi, N. and Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351–358.
  14. Molders, P., Hogberg, J. and Omenivs, S. Isolation and use of liver cells. Methods Enzymol. 1978; 52: 60–71.
  15. Kakkar, P., Das, B. and Viswanathan, P.N. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 1984; 21: 130–132.
  16. Aebi, H. and Bergmeyer, H.U. Methods of Enzymatic Analysis. 2nd ed. Chemic Academic Press Inc, Verlag 1974; 2: 673-685.
  17. Ellman, G.L. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82: 70–77.
  18. Wendel, A. Glutathione peroxidase. Methods Enzymol. 1981; 77: 325–333.
  19. Jiankang, L., Rei, E., Hideaki, K. and Akitane, M. Antioxidant action of Guilingji in the brain of rats with FeCl3-induced epilepsy. Free Radic Biol Med. 1990; 9: 451–454.
  20. Annadora, J.B. and Michel, B. Oxygen free radicals in rat limbic structure after kainate-induced seizures. Free Radic Biol Med. 1995; 18: 993–1002.
Read More

Author Biographies

P. Xiao, Department of cardiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China

K. Zhang, Department of cardiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China

Z. Tao, Department of cardiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China

N. Liu, Department of cardiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China

B. Ge, Central laboratory, Nanjing first hospital, Nanjing Medical University, Nanjing 211100, China

Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 935 Download : 402