Issue
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
MiRNA-378 controls cell proliferation in rabbit umbilical cord mesenymal stem cells
Corresponding Author(s) : H. Yu
phdwanghongcheng@126.com
Cellular and Molecular Biology,
Vol. 62 No. 12: Issue 12
Abstract
Mesenchymal stem cells (MSC) are known to have the ability to differentiate into various lineages of mesenchymal tissue. They are widely distributed in a variety of tissues in the body and are also present in the foetal environment. MicroRNAs (miRNAs) are a fundamental class of biological molecules that play a crucial role in development. In this study, microRNA-378 and its predicted target (Sufu, suppressor of fused homolog) were used to study proliferation of rabbit umbilical cord mesenymal stem cells, luciferase reporter assay was used to assess and confirm the binding sequence of 3"²untranslated region between microRNA-378 and Sufu. The results showed that miRNA-378 overexpression reduced Sufu gene, and promoted cell proliferation of rabbit umbilical cord mesenymal stem cells via cell viability and BrdU testing, which molecular mechanisms were down-regulation of apoptosis genes after miRNA-378 overexpression. In conclusion, our research shows that the microRNA-378 promoted the cell proliferation of rabbit umbilical cord mesenymal stem cells, and this mechanism was miRNA-378 reduced the expression of Sufu, increased cell proliferation.
Keywords
microRNA
cell proliferation
Mesenymal Stem Cells
Umbilical Cord.
Wang, H., Zhao, Z., Du, J., Wang, T., Yang, L., & Yu, H. (2016). MiRNA-378 controls cell proliferation in rabbit umbilical cord mesenymal stem cells. Cellular and Molecular Biology, 62(12), 97–101. https://doi.org/10.14715/cmb/2016.62.12.17
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX