Oncolytic E1B 55KDa-deleted adenovirus replication is independent of p53 levels in cancer cells

B. M. Abbas; M. A. El-Mogy; Y. Haj-Ahmad

doi:10.14715/cmb/2017.63.7.1

Issue

Vol. 63 No. 7: Issue 7

Issue Published : August 22, 2017

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Oncolytic E1B 55KDa-deleted adenovirus replication is independent of p53 levels in cancer cells

https://doi.org/10.14715/cmb/2017.63.7.1

B. M. Abbas

Centre for Biotechnology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, L2S 3A1, Canada

M. A. El-Mogy

Molecular Biology Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza, Egypt, P.O. 12622

Y. Haj-Ahmad

Department of Biological Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, L2S 3A1, Canada

Corresponding Author(s) : B. M. Abbas

bma_1403@hotmail.com

Cellular and Molecular Biology, Vol. 63 No. 7: Issue 7
Article Published : August 15, 2017

Abstract

Oncolytic adenoviruses represent a new approach for cancer therapy due to its tumor specificity. E1B 55kDa-deleted adenovirus type 5 (Ad5dlE1B 55kDa) is a promising therapeutic agent that can selectively replicate in and lyse p53 defective cancer cells. However, the overall efficacy has shown varying degrees of success with raised doubts about the correlation between p53 status and E1B-deleted adenovirus replication ability. In this study, we investigated the relationship between the efficiency of Ad5dlE1B 55kDa replication and p53 levels in cancer cells. Five transient p53 expression vectors were engineered to expresses different p53 levels in transfected cells. Then, the effect of the variable p53 levels and cellular backgrounds on the replication efficiency of oncolytic Ad5dlE1B 55kDa was evaluated in H1299 and HeLa cell lines. We found that the replication efficiency of these oncolytic viruses is dependent on the status, but not the expression levels, of p53. Ad5dlE1B 55kDa was shown to have selective replication activity in H1299 cells (p53-null) and decreased viral replication in HeLa cells (p53-positive), relative to the wild-type adenovirus in both cell lines. Our findings suggest that there is a relation between the E1B-deleted adenovirus replication and the presence as well as the activity of p53, independent of its quantity.

Keywords

Oncolytic adenovirus p53 E1B 55kDa H1299 cells HeLa cell.

Abbas, B. M., El-Mogy, M. A., & Haj-Ahmad, Y. (2017). Oncolytic E1B 55KDa-deleted adenovirus replication is independent of p53 levels in cancer cells. Cellular and Molecular Biology, 63(7), 1–11. https://doi.org/10.14715/cmb/2017.63.7.1