Functional role of inflammation in the surgical injury induced vascular remodeling of male albino rats

X. Song; L. Li; Y. Wu; L. Zhang; X. Li

doi:10.14715/cmb/2017.63.9.2

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Vol. 63 No. 9: Issue 9

Issue Published : September 30, 2017

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Functional role of inflammation in the surgical injury induced vascular remodeling of male albino rats

https://doi.org/10.14715/cmb/2017.63.9.2

X. Song

Department of general surgery, Lanzhou University first hospital, Lanzhou, Jiangsu 730000, China

L. Li

Department of general surgery, Henan energy jiaozuo coal Central Hospital, Jiaozuo, Henan 454000, china

Y. Wu

Department of general surgery, Lanzhou University first hospital, Lanzhou, Jiangsu 730000, China

L. Zhang

Department of general surgery, Lanzhou University first hospital, Lanzhou, Jiangsu 730000, China

X. Li

Department of general surgery, Lanzhou University first hospital, Lanzhou, Jiangsu 730000, China

Corresponding Author(s) : X. Li

xunli13456@hotmail.com

Cellular and Molecular Biology, Vol. 63 No. 9: Issue 9
Article Published : September 30, 2017

Abstract

Our present study investigates the cellular and molecular inflammatory events in male albino rat arterial injury. Male albino rats were subjected to longitudinal incision and carotid artery clamping for the duration of 45 days. Heat shock protein (HSP) 27, HSP70, HSP47 and Nuclear Factor kappa B (NF-ÎºB) expressions were determined by qPCR and Western blot method. The morphology of vessel wall alteration was studied by the light microscopy. The expression of NF-ÎºB was found to be increased after ten days of carotid artery injury. The qPCR and Western blot analysis showed elevation in HSP47, HSP27, and HSP70 expression, ten days following the surgical injury. The neointima-formation and the media layer discontinuity were evidenced by light microscopy. The dendritic-like cells were in close contact with the lymphocytes. Our study reports that the surgical injury induces an inflammatory response through the increased NF-ÎºB and HSPs expression.

Keywords

Inflammation Rats Vascular remodeling Protein.

Song, X., Li, L., Wu, Y., Zhang, L., & Li, X. (2017). Functional role of inflammation in the surgical injury induced vascular remodeling of male albino rats. Cellular and Molecular Biology, 63(9), 8–12. https://doi.org/10.14715/cmb/2017.63.9.2

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References

REFERENCES
Drachman, D.E. and Simon, D.I. Inflammation as a mechanism and therapeutic target for in-stent restenosis. Curr Atheroscler Rep 2005; 7: 44-49.
Aronson, D. and Edelman, E.R. Revascularization for coronary artery disease in diabetes mellitus: Angioplasty, stents, and coronary artery bypass grafting. Reviews in endocrine & metabolic disorders 2010; 11:75-86.
Schillinger, M. and Minar, E. Restenosis after Percutaneous Angioplasty: The Role of Vascular Inflammation. Vascular Health and Risk Management 2005; 1:73-78.
Toutouzas, K., Colombo, A. and Stefanadis, C. Inflammation and restenosis after percutaneous coronary interventions. Eur Heart J 2004; 25: 1679-1687.
Shah PK: Inflammation, Neointimal Hyperplasia, and Restenosis As the Leukocytes Roll, the Arteries Thicken. Circulation 107:2175-2177, 2003.
Bhatt, D.L. Inflammation and Restenosis: is there a link? Am Heart J 2004; 147: 945-947.
Steffens, S. and Mach, F. Inflammation and atherosclerosis. Herz 2004; 29: 741-748.
Oeckinghaus, A. and Ghosh, S. The NF-ÎºB Family of Transcription Factors and Its Regulation. Cold Spring Harbor Perspectives in Biology 2009; 1(4): a000034.
Greenberg, G.J.S., Barshack, I., Singh, M., Prichen, S., Laniado, S. and Keren, G. Accelerated intimal thickening in carotid arteries of balloon-injured rats after immunization against heat shock protein 70. J Am Coll Cardiol 2001; 38: 1564-1569.
Pockely, A.G. Heat shock proteins, inflammation, and cardiovascular disease. Circulation 2002; 105: 1012-1017.
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Snoeckx, L.H.E.H., Cornelussen, R.N., Van Nieuwenhoven, F.A., Reneman, R.S. and Van Der Vusse, G.J. Heat shock proteins and cardiovascular pathophysiology. Physiol Rev 2001; 81: 1461-1497.
Kong, D., Melo, L.G., Gnecchi, M., Zhang, L., Mostoslavsky, G., Liew, C.C., Pratt, R.E. and Dzau, V.J. Cytokine-induced mobilization of circulating endothelial progenitor cells enhances repair of injured arteries. Circulation 2004; 110: 2039-2046,.
Takahashi, T. and Lee, R.T. Dendritic cells in neointima formation: from where did you come, and what are you doing here? J Am Coll Cardiol 2003; 42: 939-941.
Muthuraman, P., Ramkumar, K. and Kim, D.H. Analysis of the dose-dependent effect of zinc oxide nanoparticles on the oxidative stress and antioxidant enzyme activity in adipocytes. Appl Biochem Biotech 2014; 174(8): 2851-63.
Muthuraman, P., Jeong Eun, P. and Eunjung, K. Aspartame down-regulates 3T3-L1 differentiation. In Vitro Cellular & Developmental Biology-Animal 2014; 50: 851-857.
Van 't Klooster, R., Truijman, M.T., van Dijk, A.C., Schreuder, F.H., Kooi, M.E., van der Lugt, A. and van der Geest, R.J. Visualization of local changes in vessel wall morphology and plaque progression in serial carotid artery magnetic resonance imaging. Stroke 2014; 45(8): e160-3.
Hiscott, J., Kwon, H. and Génin, P. Hostile takeovers: viral appropriation of the NF-kB pathway. Journal of Clinical Investigation 2001; 107(2):143-151.
Liu, H.S., Pan, C.E., Liu, Q.G., Yang, W. and Liu, X.M. Effect of NF-kB and p38 MAPK in activated monocytes/macrophages on pro-inflammatory cytokines of rats with acute pancreatitis. World J. Gastroenterol 2003; 9: 2513-2518.
Lu, X. and Kakkar, V. The role of heat shock protein (HSP) in atherosclerosis: Pathophysiology and clinical opportunities. Curr Med Chem 2010; 17(10): 957-973.
Xu, Q., Li, D., Holbrook, N.J. and Udelsman, R. Acute hypertension induces heat- shock protein 70 gene expression in rat aorta. Circulation 1995; 92: 1223-1229.
Connolly, E.M., Kelly, C.J., Chen, G., O'Grady, T., Kay, E., Leahy, A. and Bouchier-Hayes, D.J. Pharmacological induction of hsp27 attenuates intimal hyperplasia in vivo. Eur J Vasc Endovasc Surg 2003; 25: 40-47.
Sauk, J.J., Nikitakis, N. and Siavash, H. Hsp47, a novel collagen, binding serpin chaperone, autoantigen, and therapeutic target. Front Biosci 2005; 10: 107-118.
Sluijter, J.P., Smeets, M.B., Velema, E., Pasterkamp, G. and De Klein, D.P. Increase in collagen fiber content is associated with flow-induced arterial remodeling. J Vasc Res 2004; 41: 546-555.
Schell, M.T., Spitzer, A.L., Johnson, J.A., Lee, D. and Harris, H.W. Heat shock inhibits NF-kB activation in a dose- and time-dependent manner. J Surg Res 2005; 129: 90-93.
Sata, M., Saiura, A., Kunisato, A., Tojo, A., Okada, S., Tokuhisa, T., Hirai, H., Makuuch, M., Hirata, Y. and Nagai, R. Hematopoietic stem cells differentiate into vascular cells that participate in the pathogenesis of atherosclerosis. Nature Med 2002; 8: 403-409.
Bailey, A.S. and Fleming, W.H. Converging roads: evidence for an adult hemangioblast. Exp Hematol 2003; 31: 987-993.

References

REFERENCES

Drachman, D.E. and Simon, D.I. Inflammation as a mechanism and therapeutic target for in-stent restenosis. Curr Atheroscler Rep 2005; 7: 44-49.

Aronson, D. and Edelman, E.R. Revascularization for coronary artery disease in diabetes mellitus: Angioplasty, stents, and coronary artery bypass grafting. Reviews in endocrine & metabolic disorders 2010; 11:75-86.

Schillinger, M. and Minar, E. Restenosis after Percutaneous Angioplasty: The Role of Vascular Inflammation. Vascular Health and Risk Management 2005; 1:73-78.

Toutouzas, K., Colombo, A. and Stefanadis, C. Inflammation and restenosis after percutaneous coronary interventions. Eur Heart J 2004; 25: 1679-1687.

Shah PK: Inflammation, Neointimal Hyperplasia, and Restenosis As the Leukocytes Roll, the Arteries Thicken. Circulation 107:2175-2177, 2003.

Bhatt, D.L. Inflammation and Restenosis: is there a link? Am Heart J 2004; 147: 945-947.

Steffens, S. and Mach, F. Inflammation and atherosclerosis. Herz 2004; 29: 741-748.

Oeckinghaus, A. and Ghosh, S. The NF-ÎºB Family of Transcription Factors and Its Regulation. Cold Spring Harbor Perspectives in Biology 2009; 1(4): a000034.

Greenberg, G.J.S., Barshack, I., Singh, M., Prichen, S., Laniado, S. and Keren, G. Accelerated intimal thickening in carotid arteries of balloon-injured rats after immunization against heat shock protein 70. J Am Coll Cardiol 2001; 38: 1564-1569.

Pockely, A.G. Heat shock proteins, inflammation, and cardiovascular disease. Circulation 2002; 105: 1012-1017.

Yoshimura, S., Morishita, R., Hayashi, K., Yamamoto, K., Nakagami, H., Kaneda, Y., Sakai, N. and Ogihara, T. Inhibition of intimal hyperplasia after balloon injury in rat carotid artery model using cis-element ‘decoy' of the nuclear factor-kappaB binding site as a novel molecular strategy. Gene Therapy 2001; 8:1635-1642.

Snoeckx, L.H.E.H., Cornelussen, R.N., Van Nieuwenhoven, F.A., Reneman, R.S. and Van Der Vusse, G.J. Heat shock proteins and cardiovascular pathophysiology. Physiol Rev 2001; 81: 1461-1497.

Kong, D., Melo, L.G., Gnecchi, M., Zhang, L., Mostoslavsky, G., Liew, C.C., Pratt, R.E. and Dzau, V.J. Cytokine-induced mobilization of circulating endothelial progenitor cells enhances repair of injured arteries. Circulation 2004; 110: 2039-2046,.

Takahashi, T. and Lee, R.T. Dendritic cells in neointima formation: from where did you come, and what are you doing here? J Am Coll Cardiol 2003; 42: 939-941.

Muthuraman, P., Ramkumar, K. and Kim, D.H. Analysis of the dose-dependent effect of zinc oxide nanoparticles on the oxidative stress and antioxidant enzyme activity in adipocytes. Appl Biochem Biotech 2014; 174(8): 2851-63.

Muthuraman, P., Jeong Eun, P. and Eunjung, K. Aspartame down-regulates 3T3-L1 differentiation. In Vitro Cellular & Developmental Biology-Animal 2014; 50: 851-857.

Van 't Klooster, R., Truijman, M.T., van Dijk, A.C., Schreuder, F.H., Kooi, M.E., van der Lugt, A. and van der Geest, R.J. Visualization of local changes in vessel wall morphology and plaque progression in serial carotid artery magnetic resonance imaging. Stroke 2014; 45(8): e160-3.

Hiscott, J., Kwon, H. and Génin, P. Hostile takeovers: viral appropriation of the NF-kB pathway. Journal of Clinical Investigation 2001; 107(2):143-151.

Liu, H.S., Pan, C.E., Liu, Q.G., Yang, W. and Liu, X.M. Effect of NF-kB and p38 MAPK in activated monocytes/macrophages on pro-inflammatory cytokines of rats with acute pancreatitis. World J. Gastroenterol 2003; 9: 2513-2518.

Lu, X. and Kakkar, V. The role of heat shock protein (HSP) in atherosclerosis: Pathophysiology and clinical opportunities. Curr Med Chem 2010; 17(10): 957-973.

Xu, Q., Li, D., Holbrook, N.J. and Udelsman, R. Acute hypertension induces heat- shock protein 70 gene expression in rat aorta. Circulation 1995; 92: 1223-1229.

Connolly, E.M., Kelly, C.J., Chen, G., O'Grady, T., Kay, E., Leahy, A. and Bouchier-Hayes, D.J. Pharmacological induction of hsp27 attenuates intimal hyperplasia in vivo. Eur J Vasc Endovasc Surg 2003; 25: 40-47.

Sauk, J.J., Nikitakis, N. and Siavash, H. Hsp47, a novel collagen, binding serpin chaperone, autoantigen, and therapeutic target. Front Biosci 2005; 10: 107-118.

Sluijter, J.P., Smeets, M.B., Velema, E., Pasterkamp, G. and De Klein, D.P. Increase in collagen fiber content is associated with flow-induced arterial remodeling. J Vasc Res 2004; 41: 546-555.

Schell, M.T., Spitzer, A.L., Johnson, J.A., Lee, D. and Harris, H.W. Heat shock inhibits NF-kB activation in a dose- and time-dependent manner. J Surg Res 2005; 129: 90-93.

Sata, M., Saiura, A., Kunisato, A., Tojo, A., Okada, S., Tokuhisa, T., Hirai, H., Makuuch, M., Hirata, Y. and Nagai, R. Hematopoietic stem cells differentiate into vascular cells that participate in the pathogenesis of atherosclerosis. Nature Med 2002; 8: 403-409.

Bailey, A.S. and Fleming, W.H. Converging roads: evidence for an adult hemangioblast. Exp Hematol 2003; 31: 987-993.

Author biographies is not available.