Mass spectrometric identification of diagnostic markers for chronic prostatitis in seminal plasma by analysis of seminal plasma protein clinical samples

A. Rokka; A. Mehik; P. Tonttila; M. Vaarala

doi:10.14715/cmb/2017.63.7.6

Issue

Vol. 63 No. 7: Issue 7

Issue Published : August 22, 2017

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Mass spectrometric identification of diagnostic markers for chronic prostatitis in seminal plasma by analysis of seminal plasma protein clinical samples

https://doi.org/10.14715/cmb/2017.63.7.6

A. Rokka

Turku Centre for Biotechnology, University of Turku and í…bo Akademi University, Turku, Finland

A. Mehik

Division of Operative Care and Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Kajaanintie 50, 90220 Oulu, Finland

P. Tonttila

Division of Operative Care and Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Kajaanintie 50, 90220 Oulu, Finland

M. Vaarala

Division of Operative Care and Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Kajaanintie 50, 90220 Oulu, Finland

Corresponding Author(s) : M. Vaarala

markku.vaarala@oulu.fi

Cellular and Molecular Biology, Vol. 63 No. 7: Issue 7
Article Published : August 15, 2017

Abstract

There are few specific diagnostic markers for chronic prostatitis. Therefore, we used mass spectrometry to evaluate differences in seminal plasma protein expression among patients with prostatitis and young and middle-aged healthy controls. We analysed pooled seminal plasma protein samples from four prostatitis patients (two pools), three young controls (one pool), and three middle-aged controls (one pool). The samples were analysed by liquid chromatography–tandem mass spectrometry. Of the 349 proteins identified, 16 were differentially expressed between the two control pools. Five proteins were up- or down-regulated in both of the prostatitis pools compared to middle-aged controls but not between young and middle-aged pools. Progestagen-associated endometrial protein (PAEP) was over-expressed in prostatitis samples compared to young and middle-aged controls. Our findings and those of previous studies indicate that PAEP is a potential seminal plasma marker for chronic prostatitis. In conclusion, we found age-related changes in seminal plasma protein expression. PAEP expression in seminal plasma should be investigated further to evaluate its potential as a diagnostic marker for chronic prostatitis.

Keywords

Prostatitis Semen Mass spectrometry.

Rokka, A., Mehik, A., Tonttila, P., & Vaarala, M. (2017). Mass spectrometric identification of diagnostic markers for chronic prostatitis in seminal plasma by analysis of seminal plasma protein clinical samples. Cellular and Molecular Biology, 63(7), 35–39. https://doi.org/10.14715/cmb/2017.63.7.6

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References

Mehik A, Hellstrom P, Lukkarinen O, Sarpola A, Jarvelin M. Epidemiology of prostatitis in Finnish men: a population-based cross-sectional study. BJU Int 2000; 86:443-8.
Mehik A, Hellstrom P, Sarpola A, Lukkarinen O, Jarvelin MR. Fears, sexual disturbances and personality features in men with prostatitis: a population-based cross-sectional study in Finland. BJU Int 2001; 88:35-8.
Magistro G, Wagenlehner FM, Grabe M, Weidner W, Stief CG, Nickel JC. Contemporary Management of Chronic Prostatitis/Chronic Pelvic Pain Syndrome. Eur Urol 2016; 69:286-97.
Schaeffer AJ, Knauss JS, Landis JR, Propert KJ, Alexander RB, Litwin MS, et al. Leukocyte and bacterial counts do not correlate with severity of symptoms in men with chronic prostatitis: the National Institutes of Health Chronic Prostatitis Cohort Study. J Urol 2002; 168:1048-53.
Punab M, Loivukene K, Kermes K, Mandar R. The limit of leucocytospermia from the microbiological viewpoint. Andrologia 2003; 35:271-8.
Schneider H, Ludwig M, Hossain HM, Diemer T, Weidner W. The 2001 Giessen Cohort Study on patients with prostatitis syndrome--an evaluation of inflammatory status and search for microorganisms 10 years after a first analysis. Andrologia 2003; 35:258-62.
Korrovits P, Punab M, Turk S, Mandar R. Seminal microflora in asymptomatic inflammatory (NIH IV category) prostatitis. Eur Urol 2006; 50:1338,44; discussion 1344-6.
Paulis G, Conti E, Voliani S, Bertozzi MA, Sarteschi ML, Menchini Fabris F. Evaluation of the cytokines in genital secretions of patients with chronic prostatitis. Arch Ital Urol Androl 2003; 75:179-86.
Korrovits P, Ausmees K, Mandar R, Punab M. Seminal interleukin-6 and serum prostate-specific antigen as possible predictive biomarkers in asymptomatic inflammatory prostatitis. Urology 2011; 78:442-6.
John H, Maake C, Barghorn A, Zbinden R, Hauri D, Joller-Jemelka HI. Immunological alterations in the ejaculate of chronic prostatitis patients: clues for autoimmunity. Andrologia 2003; 35:294-9.
Penna G, Mondaini N, Amuchastegui S, Degli Innocenti S, Carini M, Giubilei G, et al. Seminal plasma cytokines and chemokines in prostate inflammation: interleukin 8 as a predictive biomarker in chronic prostatitis/chronic pelvic pain syndrome and benign prostatic hyperplasia. Eur Urol 2007; 51:524,33; discussion 533.
Khadra A, Fletcher P, Luzzi G, Shattock R, Hay P. Interleukin-8 levels in seminal plasma in chronic prostatitis/chronic pelvic pain syndrome and nonspecific urethritis. BJU Int 2006; 97:1043-6.
Miller LJ, Fischer KA, Goralnick SJ, Litt M, Burleson JA, Albertsen P, et al. Interleukin-10 levels in seminal plasma: implications for chronic prostatitis-chronic pelvic pain syndrome. J Urol 2002; 167:753-6.
Kastner C, Jakse G. Measurement of immunoglobulins in seminal fluid with modified nephelometry--an alternative diagnostic tool for chronic prostatitis. Prostate Cancer Prostatic Dis 2003; 6:86-9.
Ludwig M, Vidal A, Diemer T, Pabst W, Failing K, Weidner W. Chronic prostatitis/chronic pelvic pain pyndrome): seminal markers of inflammation. World J Urol 2003; 21:82-5.
Miller LJ, Fischer KA, Goralnick SJ, Litt M, Burleson JA, Albertsen P, et al. Nerve growth factor and chronic prostatitis/chronic pelvic pain syndrome. Urology 2002; 59:603-8.
Rolland AD, Lavigne R, Dauly C, Calvel P, Kervarrec C, Freour T, et al. Identification of genital tract markers in the human seminal plasma using an integrative genomics approach. Hum Reprod 2013; 28:199-209.
Wang J, Wang J, Zhang HR, Shi HJ, Ma D, Zhao HX, et al. Proteomic analysis of seminal plasma from asthenozoospermia patients reveals proteins that affect oxidative stress responses and semen quality. Asian J Androl 2009; 11:484-91.
Batruch I, Lecker I, Kagedan D, Smith CR, Mullen BJ, Grober E, et al. Proteomic analysis of seminal plasma from normal volunteers and post-vasectomy patients identifies over 2000 proteins and candidate biomarkers of the urogenital system. J Proteome Res 2011; 10:941-53.
Camargo M, Lopes PI, Del Giudice PT, Carvalho VM, Cardozo KH, Andreoni C, et al. Unbiased label-free quantitative proteomic profiling and enriched proteomic pathways in seminal plasma of adult men before and after varicocelectomy. Hum Reprod 2013; 28:33-46.
Kagedan D, Lecker I, Batruch I, Smith C, Kaploun I, Lo K, et al. Characterization of the seminal plasma proteome in men with prostatitis by mass spectrometry. Clin Proteomics 2012; 9:2,0275-9-2.
Shilov IV, Seymour SL, Patel AA, Loboda A, Tang WH, Keating SP, et al. The Paragon Algorithm, a next generation search engine that uses sequence temperature values and feature probabilities to identify peptides from tandem mass spectra. Mol Cell Proteomics 2007; 6:1638-55.
Kidd SA, Eskenazi B, Wyrobek AJ. Effects of male age on semen quality and fertility: a review of the literature. Fertil Steril 2001; 75:237-48.
Eskenazi B, Wyrobek AJ, Sloter E, Kidd SA, Moore L, Young S, et al. The association of age and semen quality in healthy men. Hum Reprod 2003; 18:447-54.
Song M, Ramaswamy S, Ramachandran S, Flowers LC, Horowitz IR, Rock JA, et al. Angiogenic role for glycodelin in tumorigenesis. Proc Natl Acad Sci U S A 2001; 98:9265-70.
Morris HR, Dell A, Easton RL, Panico M, Koistinen H, Koistinen R, et al. Gender-specific glycosylation of human glycodelin affects its contraceptive activity. J Biol Chem 1996; 271:32159-67.
Cohen JM, Fagin AP, Hariton E, Niska JR, Pierce MW, Kuriyama A, et al. Therapeutic intervention for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS): a systematic review and meta-analysis. PLoS One 2012; 7:e41941.
Anothaisintawee T, Attia J, Nickel JC, Thammakraisorn S, Numthavaj P, McEvoy M, et al. Management of chronic prostatitis/chronic pelvic pain syndrome: a systematic review and network meta-analysis. Jama 2011; 305:78-86.
Thakkinstian A, Attia J, Anothaisintawee T, Nickel JC. Alpha-Blockers, Antibiotics and Anti-Inflammatories have a Role in the Management of Chronic Prostatitis/chronic Pelvic Pain Syndrome. BJU Int 2012; 110:1014-22.

References

Mehik A, Hellstrom P, Lukkarinen O, Sarpola A, Jarvelin M. Epidemiology of prostatitis in Finnish men: a population-based cross-sectional study. BJU Int 2000; 86:443-8.

Mehik A, Hellstrom P, Sarpola A, Lukkarinen O, Jarvelin MR. Fears, sexual disturbances and personality features in men with prostatitis: a population-based cross-sectional study in Finland. BJU Int 2001; 88:35-8.

Magistro G, Wagenlehner FM, Grabe M, Weidner W, Stief CG, Nickel JC. Contemporary Management of Chronic Prostatitis/Chronic Pelvic Pain Syndrome. Eur Urol 2016; 69:286-97.

Schaeffer AJ, Knauss JS, Landis JR, Propert KJ, Alexander RB, Litwin MS, et al. Leukocyte and bacterial counts do not correlate with severity of symptoms in men with chronic prostatitis: the National Institutes of Health Chronic Prostatitis Cohort Study. J Urol 2002; 168:1048-53.

Punab M, Loivukene K, Kermes K, Mandar R. The limit of leucocytospermia from the microbiological viewpoint. Andrologia 2003; 35:271-8.

Schneider H, Ludwig M, Hossain HM, Diemer T, Weidner W. The 2001 Giessen Cohort Study on patients with prostatitis syndrome--an evaluation of inflammatory status and search for microorganisms 10 years after a first analysis. Andrologia 2003; 35:258-62.

Korrovits P, Punab M, Turk S, Mandar R. Seminal microflora in asymptomatic inflammatory (NIH IV category) prostatitis. Eur Urol 2006; 50:1338,44; discussion 1344-6.

Paulis G, Conti E, Voliani S, Bertozzi MA, Sarteschi ML, Menchini Fabris F. Evaluation of the cytokines in genital secretions of patients with chronic prostatitis. Arch Ital Urol Androl 2003; 75:179-86.

Korrovits P, Ausmees K, Mandar R, Punab M. Seminal interleukin-6 and serum prostate-specific antigen as possible predictive biomarkers in asymptomatic inflammatory prostatitis. Urology 2011; 78:442-6.

John H, Maake C, Barghorn A, Zbinden R, Hauri D, Joller-Jemelka HI. Immunological alterations in the ejaculate of chronic prostatitis patients: clues for autoimmunity. Andrologia 2003; 35:294-9.

Penna G, Mondaini N, Amuchastegui S, Degli Innocenti S, Carini M, Giubilei G, et al. Seminal plasma cytokines and chemokines in prostate inflammation: interleukin 8 as a predictive biomarker in chronic prostatitis/chronic pelvic pain syndrome and benign prostatic hyperplasia. Eur Urol 2007; 51:524,33; discussion 533.

Khadra A, Fletcher P, Luzzi G, Shattock R, Hay P. Interleukin-8 levels in seminal plasma in chronic prostatitis/chronic pelvic pain syndrome and nonspecific urethritis. BJU Int 2006; 97:1043-6.

Miller LJ, Fischer KA, Goralnick SJ, Litt M, Burleson JA, Albertsen P, et al. Interleukin-10 levels in seminal plasma: implications for chronic prostatitis-chronic pelvic pain syndrome. J Urol 2002; 167:753-6.

Kastner C, Jakse G. Measurement of immunoglobulins in seminal fluid with modified nephelometry--an alternative diagnostic tool for chronic prostatitis. Prostate Cancer Prostatic Dis 2003; 6:86-9.

Ludwig M, Vidal A, Diemer T, Pabst W, Failing K, Weidner W. Chronic prostatitis/chronic pelvic pain pyndrome): seminal markers of inflammation. World J Urol 2003; 21:82-5.

Miller LJ, Fischer KA, Goralnick SJ, Litt M, Burleson JA, Albertsen P, et al. Nerve growth factor and chronic prostatitis/chronic pelvic pain syndrome. Urology 2002; 59:603-8.

Rolland AD, Lavigne R, Dauly C, Calvel P, Kervarrec C, Freour T, et al. Identification of genital tract markers in the human seminal plasma using an integrative genomics approach. Hum Reprod 2013; 28:199-209.

Wang J, Wang J, Zhang HR, Shi HJ, Ma D, Zhao HX, et al. Proteomic analysis of seminal plasma from asthenozoospermia patients reveals proteins that affect oxidative stress responses and semen quality. Asian J Androl 2009; 11:484-91.

Batruch I, Lecker I, Kagedan D, Smith CR, Mullen BJ, Grober E, et al. Proteomic analysis of seminal plasma from normal volunteers and post-vasectomy patients identifies over 2000 proteins and candidate biomarkers of the urogenital system. J Proteome Res 2011; 10:941-53.

Camargo M, Lopes PI, Del Giudice PT, Carvalho VM, Cardozo KH, Andreoni C, et al. Unbiased label-free quantitative proteomic profiling and enriched proteomic pathways in seminal plasma of adult men before and after varicocelectomy. Hum Reprod 2013; 28:33-46.

Kagedan D, Lecker I, Batruch I, Smith C, Kaploun I, Lo K, et al. Characterization of the seminal plasma proteome in men with prostatitis by mass spectrometry. Clin Proteomics 2012; 9:2,0275-9-2.

Shilov IV, Seymour SL, Patel AA, Loboda A, Tang WH, Keating SP, et al. The Paragon Algorithm, a next generation search engine that uses sequence temperature values and feature probabilities to identify peptides from tandem mass spectra. Mol Cell Proteomics 2007; 6:1638-55.

Kidd SA, Eskenazi B, Wyrobek AJ. Effects of male age on semen quality and fertility: a review of the literature. Fertil Steril 2001; 75:237-48.

Eskenazi B, Wyrobek AJ, Sloter E, Kidd SA, Moore L, Young S, et al. The association of age and semen quality in healthy men. Hum Reprod 2003; 18:447-54.

Song M, Ramaswamy S, Ramachandran S, Flowers LC, Horowitz IR, Rock JA, et al. Angiogenic role for glycodelin in tumorigenesis. Proc Natl Acad Sci U S A 2001; 98:9265-70.

Morris HR, Dell A, Easton RL, Panico M, Koistinen H, Koistinen R, et al. Gender-specific glycosylation of human glycodelin affects its contraceptive activity. J Biol Chem 1996; 271:32159-67.

Cohen JM, Fagin AP, Hariton E, Niska JR, Pierce MW, Kuriyama A, et al. Therapeutic intervention for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS): a systematic review and meta-analysis. PLoS One 2012; 7:e41941.

Anothaisintawee T, Attia J, Nickel JC, Thammakraisorn S, Numthavaj P, McEvoy M, et al. Management of chronic prostatitis/chronic pelvic pain syndrome: a systematic review and network meta-analysis. Jama 2011; 305:78-86.

Thakkinstian A, Attia J, Anothaisintawee T, Nickel JC. Alpha-Blockers, Antibiotics and Anti-Inflammatories have a Role in the Management of Chronic Prostatitis/chronic Pelvic Pain Syndrome. BJU Int 2012; 110:1014-22.

Author biographies is not available.