Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach

A. Feki; H. Ben Saad; I. Jaballi; C. Magne; O. Boudawara; K. M. Zeghal; A. Hakim; Y. Ben Ali; I. Ben Amara

doi:10.14715/cmb/2017.63.2.4

Issue

Vol. 63 No. 2: Issue 2

Issue Published : March 23, 2017

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Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach

https://doi.org/10.14715/cmb/2017.63.2.4

A. Feki

Higher Institute of Biotechnology of Sfax, 3000 Sfax, Sfax University, Tunisia

H. Ben Saad

Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, 3029 Sfax, University of Sfax , Tunisia

I. Jaballi

Higher Institute of Biotechnology of Sfax, 3000 Sfax, Sfax University, Tunisia

C. Magne

EA 2219 Géoarchitecture, University of Western Brittany, UFR Sciences & Techniques, 6 Avenue V. Le Gorgeu, CS 93 837, 29238 Brest Cedex 3, France

O. Boudawara

Anatomopathology Laboratory, CHU Habib Bourguiba 3029. Sfax University. Tunisia

K. M. Zeghal

Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, 3029 Sfax, University of Sfax , Tunisia

A. Hakim

Laboratory of Pharmacology, UR/12 ES-13, Faculty of Medicine, 3029 Sfax, University of Sfax , Tunisia

Y. Ben Ali

Laboratory of Biochemistry and Enzymatic Engineering of Lipases. BP3038-1173, Sfax University, Tunisia

I. Ben Amara

Higher Institute of Biotechnology of Sfax, 3000 Sfax, Sfax University, Tunisia

Corresponding Author(s) : A. Feki

ibtissembenamara2010@gmail.com

Cellular and Molecular Biology, Vol. 63 No. 2: Issue 2
Article Published : February 28, 2017

Abstract

The aim of this study was to elucidate the redox effects of Thiophanate methyl (MT) in the rat liver and kidney. Our results showed, after 3 days of MT injection (700 mg/kg), an increase in malondialdehyde (MDA), hydrogen peroxide and advanced oxidation protein products levels. Glutathione peroxidase and superoxide dismutase activities were also remarkably increased in the liver but decrease in the kidney. Glutathione and vitamin C values were significantly reduced. The changes in biochemical parameters were substantiated by histological and molecular data. A smear without ladder formation on agarose gel was shown, indicating random DNA degradation in the liver and the kidney of MT treated rats. The increase in cyclooxygenase-2 gene expression, marker of inflammation, and an increase in genes expression of glutathione peroxidase and superoxide dismutase in liver and their decrease in the kidney were also occurred after MT exposure. These data confirmed the pro-oxidant and genotoxic effects of this fungicide.

Keywords

Thiophanate-methyl Genotoxicity Rat Oxidative stress.

Feki, A., Ben Saad, H., Jaballi, I., Magne, C., Boudawara, O., Zeghal, K. M., Hakim, A., Ben Ali, Y., & Ben Amara, I. (2017). Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach. Cellular and Molecular Biology, 63(2), 20–28. https://doi.org/10.14715/cmb/2017.63.2.4