Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization

H. Tebyanian; A. Karami; E. Motavallian; J. Aslani; A. Samadikuchaksaraei; B. Arjmand; M. R. Nourani

doi:10.14715/cmb/2017.63.7.8

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Vol. 63 No. 7: Issue 7

Issue Published : August 22, 2017

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Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization

https://doi.org/10.14715/cmb/2017.63.7.8

H. Tebyanian

Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

A. Karami

Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

E. Motavallian

Department of General Surgery, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran

J. Aslani

Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences; Tehran, Iran.

A. Samadikuchaksaraei

Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.

B. Arjmand

Cell Therapy and Regenerative Medicine Research Center, Endicrinology and Metabolism Cellular-Molecular Sciences Institute, Tehran Univertsity of Medical Sciences, Tehran, Iran

M. R. Nourani

Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences; Tehran, Iran.

Corresponding Author(s) : M. R. Nourani

r.nourani@yahoo.com

Cellular and Molecular Biology, Vol. 63 No. 7: Issue 7
Article Published : August 15, 2017

Abstract

Pulmonary diseases cusecs a large portion of mortality in the world. There is no more cure for pulmonary diseases and many approaches are needed for finding ways to cure. Nowadays, implantation and drugs are only ways for curing those people who are facing with these diseases. Tissue engineering and regenerative medicine have been appeared as multidisciplinary field and also, they presents new therapeutic approaches for pulmonary diseases. One of these therapeutic approaches is decellularization which removes cellular but leaves intact important extracellular matrix (ECM) proteins and three-dimensional (3D) architecture and also, this approach has been studied for in-vitro and ex-vivo. In this study, we aimed to investigate a comparison of different concentrations of Triton X-100 and Sodium dodecyl sulfate detergents in lung decellularization in order to evaluate the effects of different concentrations and times of mentioned detergents on three dimensional and ECM proteins lung. Two detergents (Triton-X100 and Sodium dodecyl sulfat) were used with different concentrations for decellularizing rat lungs for maintaining of three-dimensional lung architecture and ECM protein compositions which have significant roles in differentiation and migration of stem cells. Results showed that SDS 0.05%, 0.1% and Triton-X100 0.1% could maintain 3D, elastin and collagen better than other concentrations in 24 and 48 h- decellularization. We concluded that these approaches can help to achieve three-dimensional architecture and extracellular matrix of lung with minimum destruction for next step such as recellularization and in-vivo study.

Keywords

at lung Decellularization SDS Triton X-100.

Tebyanian, H., Karami, A., Motavallian, E., Aslani, J., Samadikuchaksaraei, A., Arjmand, B., & Nourani, M. R. (2017). Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization. Cellular and Molecular Biology, 63(7), 46–51. https://doi.org/10.14715/cmb/2017.63.7.8

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References

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Garcia O, Carraro G, Navarro S, Bertoncello I, McQualter J, Driscoll B, Jesudason E, and Warburton D. Cell-based therapies for lung disease. Br Med Bull 2012; 101 147-61.
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Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, and Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science (New York, N.Y.) 2010; 329 (5991): 538-541.
He M, Callanan A, Lagaras K, Steele JAM, and Stevens MM. Optimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys. Journal of Biomedical Materials Research Part B: Applied Biomaterials 2016.
He M and Callanan A. Comparison of methods for whole-organ decellularization in tissue engineering of bioartificial organs. Tissue Engineering Part B: Reviews 2012; 19 (3): 194-208.
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Ott HC, Matthiesen TS, Goh S-K, Black LD, Kren SM, Netoff TI, and Taylor DA. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 2008; 14 (2): 213-221.
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Badylak SF, Taylor D, and Uygun K. Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng 2011; 13 27-53.
Daly AB, Wallis JM, Borg ZD, Bonvillain RW, Deng B, Ballif BA, Jaworski DM, Allen GB, and Weiss DJ. Initial binding and re-cellularization of de-cellularized mouse lung scaffolds with bone marrow-derived mesenchymal stromal cells. Tissue Eng. A 2011; 18 (1-2): 1-16.
Babavalian H, Latifi AM, Shokrgozar MA, Bonakdar S, Shakeri F, and Tebyanian H. Healing Effects of Synthetic and Commercial Alginate Hydrogel Dressings on Wounds: A Comparative Study. Trauma Mon; Inpress (Inpress).
Babavalian H, latifi AM, Shokrgozar MA, Bonakdar S, Tebyanian H, and Shakeri F. Cloning and expression of recombinant human platelet-derived growth factor-BB in Pichia Pink. Cellular and Molecular Biology 2016; 62 (8): 45-51.
Karami A, Tebyanian H, Goodarzi V, and Shiri S. Planarians: an In Vivo Model for Regenerative Medicine. International journal of stem cells 2015; 8 (2): 128.
Shakeri F, Tebyanian H, Karami A, Babavalian H, and Tahmasbi MH. Effect of Topical Phenytoin on Wound Healing. Trauma Mon; Inpress (Inpress).
Maghsoudlou P, Georgiades F, Tyraskis A, Totonelli G, Loukogeorgakis SP, Orlando G, Shangaris P, Lange P, Delalande J-M, Burns AJ, Cenedese A, Sebire NJ, Turmaine M, Guest BN, Alcorn JF, Atala A, Birchall MA, Elliott MJ, Eaton S, Pierro A, Gilbert TW, and De Coppi P. Preservation of micro-architecture and angiogenic potential in a pulmonary acellular matrix obtained using intermittent intra-tracheal flow of detergent enzymatic treatment. Biomaterials 2013; 34 (28): 6638-6648.
Petersen TH, Calle EA, Colehour MB, and Niklason LE. Matrix Composition and Mechanics of Decellularized Lung Scaffolds. Cells, Tissues, Organs 2012; 195 (3): 222-231.
Petersen TH. In vitro development of engineered lung tissue. 2009.
Song JJ, Kim SS, Liu Z, Madsen JC, Mathisen DJ, Vacanti JP, and Ott HC. Enhanced In Vivo Function of Bioartificial Lungs in Rats. The Annals of Thoracic Surgery; 92 (3): 998-1006.
Wagner DE, Bonvillain RW, Jensen T, Girard ED, Bunnell BA, Finck CM, Hoffman AM, and Weiss DJ. Can stem cells be used to generate new lungs? Ex vivo lung bioengineering with decellularized whole lung scaffolds. Respirology 2013; 18 (6): 895-911.
O'Neill JD, Anfang R, Anandappa A, Costa J, Javidfar JJ, Wobma HM, Singh G, Freytes DO, Bacchetta MD, Sonett JR, and Vunjak-Novakovic G. Decellularization of Human and Porcine Lung Tissues for Pulmonary Tissue Engineering. The Annals of thoracic surgery 2013; 96 (3): 1046-1056.
Wallis JM, Borg ZD, Daly AB, Deng B, Ballif BA, Allen GB, Jaworski DM, and Weiss DJ. Comparative Assessment of Detergent-Based Protocols for Mouse Lung De-Cellularization and Re-Cellularization. Tissue Engineering. Part C, Methods 2012; 18 (6): 420-432.

References

Prakash YS, Tschumperlin DJ, and Stenmark KR. Coming to terms with tissue engineering and regenerative medicine in the lung. American Journal of Physiology - Lung Cellular and Molecular Physiology 2015; 309 (7): L625-L638.

Garcia O, Carraro G, Navarro S, Bertoncello I, McQualter J, Driscoll B, Jesudason E, and Warburton D. Cell-based therapies for lung disease. Br Med Bull 2012; 101 147-61.

Morrisey EE and Hogan BLM. Preparing for the First Breath: Genetic and Cellular Mechanisms in Lung Development. Developmental Cell 2010; 18 (1): 8-23.

Miniño AM, Murphy SL, Xu J, and Kochanek KD. Deaths: final data for 2008. National vital statistics reports: from the Centers for Disease Control and Prevention, National Center for Health Statistics, National Vital Statistics System 2011; 59 (10): 1-126.

Jensen T, Roszell B, Zang F, Girard E, Matson A, Thrall R, Jaworski DM, Hatton C, Weiss DJ, and Finck C. A Rapid Lung De-cellularization Protocol Supports Embryonic Stem Cell Differentiation In Vitro and Following Implantation. Tissue Engineering. Part C, Methods 2012; 18 (8): 632-646.

Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, and Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science (New York, N.Y.) 2010; 329 (5991): 538-541.

He M, Callanan A, Lagaras K, Steele JAM, and Stevens MM. Optimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys. Journal of Biomedical Materials Research Part B: Applied Biomaterials 2016.

He M and Callanan A. Comparison of methods for whole-organ decellularization in tissue engineering of bioartificial organs. Tissue Engineering Part B: Reviews 2012; 19 (3): 194-208.

Badylak SF. The extracellular matrix as a biologic scaffold material. Biomaterials 2007; 28 (25): 3587-3593.

Gilbert TW, Sellaro TL, and Badylak SF. Decellularization of tissues and organs. Biomaterials 2006; 27 (19): 3675-3683.

Ott HC, Matthiesen TS, Goh S-K, Black LD, Kren SM, Netoff TI, and Taylor DA. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 2008; 14 (2): 213-221.

Lwebuga-Mukasa JS, Ingbar DH, and Madri JA. Repopulation of a human alveolar matrix by adult rat type II pneumocytes in vitro: A novel system for type II pneumocyte culture. Experimental Cell Research 1986; 162 (2): 423-435.

Cortiella J, Niles J, Cantu A, Brettler A, Pham A, Vargas G, Winston S, Wang J, Walls S, and Nichols JE. Influence of Acellular Natural Lung Matrix on Murine Embryonic Stem Cell Differentiation and Tissue Formation. Tissue Engineering Part A 2010; 16 (8): 2565-2580.

Badylak SF, Taylor D, and Uygun K. Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng 2011; 13 27-53.

Daly AB, Wallis JM, Borg ZD, Bonvillain RW, Deng B, Ballif BA, Jaworski DM, Allen GB, and Weiss DJ. Initial binding and re-cellularization of de-cellularized mouse lung scaffolds with bone marrow-derived mesenchymal stromal cells. Tissue Eng. A 2011; 18 (1-2): 1-16.

Babavalian H, Latifi AM, Shokrgozar MA, Bonakdar S, Shakeri F, and Tebyanian H. Healing Effects of Synthetic and Commercial Alginate Hydrogel Dressings on Wounds: A Comparative Study. Trauma Mon; Inpress (Inpress).

Babavalian H, latifi AM, Shokrgozar MA, Bonakdar S, Tebyanian H, and Shakeri F. Cloning and expression of recombinant human platelet-derived growth factor-BB in Pichia Pink. Cellular and Molecular Biology 2016; 62 (8): 45-51.

Karami A, Tebyanian H, Goodarzi V, and Shiri S. Planarians: an In Vivo Model for Regenerative Medicine. International journal of stem cells 2015; 8 (2): 128.

Shakeri F, Tebyanian H, Karami A, Babavalian H, and Tahmasbi MH. Effect of Topical Phenytoin on Wound Healing. Trauma Mon; Inpress (Inpress).

Maghsoudlou P, Georgiades F, Tyraskis A, Totonelli G, Loukogeorgakis SP, Orlando G, Shangaris P, Lange P, Delalande J-M, Burns AJ, Cenedese A, Sebire NJ, Turmaine M, Guest BN, Alcorn JF, Atala A, Birchall MA, Elliott MJ, Eaton S, Pierro A, Gilbert TW, and De Coppi P. Preservation of micro-architecture and angiogenic potential in a pulmonary acellular matrix obtained using intermittent intra-tracheal flow of detergent enzymatic treatment. Biomaterials 2013; 34 (28): 6638-6648.

Petersen TH, Calle EA, Colehour MB, and Niklason LE. Matrix Composition and Mechanics of Decellularized Lung Scaffolds. Cells, Tissues, Organs 2012; 195 (3): 222-231.

Petersen TH. In vitro development of engineered lung tissue. 2009.

Song JJ, Kim SS, Liu Z, Madsen JC, Mathisen DJ, Vacanti JP, and Ott HC. Enhanced In Vivo Function of Bioartificial Lungs in Rats. The Annals of Thoracic Surgery; 92 (3): 998-1006.

Wagner DE, Bonvillain RW, Jensen T, Girard ED, Bunnell BA, Finck CM, Hoffman AM, and Weiss DJ. Can stem cells be used to generate new lungs? Ex vivo lung bioengineering with decellularized whole lung scaffolds. Respirology 2013; 18 (6): 895-911.

O'Neill JD, Anfang R, Anandappa A, Costa J, Javidfar JJ, Wobma HM, Singh G, Freytes DO, Bacchetta MD, Sonett JR, and Vunjak-Novakovic G. Decellularization of Human and Porcine Lung Tissues for Pulmonary Tissue Engineering. The Annals of thoracic surgery 2013; 96 (3): 1046-1056.

Wallis JM, Borg ZD, Daly AB, Deng B, Ballif BA, Allen GB, Jaworski DM, and Weiss DJ. Comparative Assessment of Detergent-Based Protocols for Mouse Lung De-Cellularization and Re-Cellularization. Tissue Engineering. Part C, Methods 2012; 18 (6): 420-432.

Author biographies is not available.