Morphological, genetic and pigment diversity of Nerium indicum Mill in Iran

F. Mohammadi; G. Sharifi-Sirchi; D. Samsampour

doi:10.14715/cmb/2017.63.9.12

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Vol. 63 No. 9: Issue 9

Issue Published : September 30, 2017

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Morphological, genetic and pigment diversity of Nerium indicum Mill in Iran

https://doi.org/10.14715/cmb/2017.63.9.12

F. Mohammadi

Horticultural Science Department, Agriculture and Natural Resources College, University of Hormozgan, Bandar Abbas, I.R. Iran

G. Sharifi-Sirchi

Horticultural Science Department, Agriculture and Natural Resources College, University of Hormozgan, Bandar Abbas, I.R. Iran

D. Samsampour

Horticultural Science Department, Agriculture and Natural Resources College, University of Hormozgan, Bandar Abbas, I.R. Iran

Corresponding Author(s) : G. Sharifi-Sirchi

sharifisirchi@yahoo.com

Cellular and Molecular Biology, Vol. 63 No. 9: Issue 9
Article Published : September 30, 2017

Abstract

The Nerium indicum Mill organs and parts of the 40 genotypes were sampled from 5 habitats of southand south east of Iran. In total, 15 morphological and pigmentvariables were measured. Analysis of variance was carried out based on completely randomized design and revealed significance differencesat p â‰¤ 0.05, 0.01 and 0.001 for the most variablesindicating a large-scale diversity among the genotypes. Cluster analysis divided the genotypes into 3 main groups. The first and second principal components had 34.73% and 18.67% of the variance, respectively. The main factors had 76.79% accumulated eigenvalue. Random amplified polymorphic DNA (RAPD) markers used to assess the population structure and genetic variation. In total, 361 polymorphic band amplified from effective 14 chosen RAPD markers. Analysis of molecular variance (AMOVA) showed 67% and 33% within and between populations genetic variation respectively. Cluster analysis by using UPGMA method divided genotypes into 6 main groups. A high cophenetic correlation coefficient (r = 0.90) was obtained. The first and second principle coordinates had 29.31% and 25.78% of the variance, respectively.

Keywords

Nerium indicum Mill Morphological and phytochemical variables Cluster analysis RAPD marker.

Mohammadi, F., Sharifi-Sirchi, G., & Samsampour, D. (2017). Morphological, genetic and pigment diversity of Nerium indicum Mill in Iran. Cellular and Molecular Biology, 63(9), 64–70. https://doi.org/10.14715/cmb/2017.63.9.12

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References

Dey P, Kumar T. Pharmacological aspects of Nerium indicum Mill: A comprehensive review. Pharmacogn Rev 2014; 8(16):156-162.
Tahraoui A, El-Hilaly J, Israili ZH, Lyoussi B. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in South-easttern Morocco (Errachidia province). J Ethnopharmacol 2007; 110:105-117.
Lans C. Ethnomedicines used in Trinidad and Tobago for reproductive problems. J Ethnobiol Ethnomed 2007; 3:13-24.
Tagarelli G, Tagarelli A, Piro A. Folk medicine used to heal malaria in Calabria (southern Italy). J Ethnobiol Ethnomed 2010; 6:27-43.
Loveless MD, Hamrick JL. Ecological determinants of genetic structure in plant populations. Annu Rev Ecol Syst 1984; 15:65-95.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990; 18:6531-6535.
Arnon DI. Copper enzyme polyphenoloxides in isolated chloroplast in Beta vulgaris. Plant physiol 1949; 24(1):1-15.
Kazemi M, Seyed Hajizadeh H. Assessment of genetic diversity of mints Iranian wild Mentha aquatic populations using RAPD markers. J Agr Tech 2012; 8(1):327-336.
Okon S, Surmacz-Magdziak A. The use of RAPD markers for detecting genetic similarity and molecular identification of chamomile (Chamomilla recutita (L.) Rausch.) genotypes. Herba Pol 2011; 57(1):38-47.
Shasany AK, Darokar MP, Dhawan S, Gupta AK, Gupta S, Shukla AK, et al. Use of RAPD and AFLP Markers to Identify Inter- and Intraspecific Hybrids of Mentha. J Hered 2005; 96(5):542-549.
RAPD 10mer KITS (KIT A) Sequences - Eurofins Genomics. http://www.eurofinsgenomics.eu.
Manuela M, Gouveia C. Genetic diversity in Hemileia vastatrix based on RAPD markers. Mycologia 2005; 97(2):396-404.
Powell W, Morgante M, Andre C, Vogel J, Tingey S, Rafalski A. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 1996; 2:225-238.
Rohlf FJ. On applications of geometric morphometrics to studies of ontogeny and phylogeny. Syst Biol 1998; 47:147– 158.
Peakall R, Smouse PE. GeneAlex 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update. Bioinformatics 2012; 28(19):2537-2539.
Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM. PopGene32, Microsoft Windows-based freeware for population genetic analysis, version 1.32. Molecular Biology and Biotechnology Center. University of Alberta, Canada. 2000.
Yuan Z, Yin Y, Qu J, Zhu L, Li Y, 2007. Population genetic diversity in Chinese pomegranate (Punica granatum L.) cultivars revealed by fluorescent-AFLP markers. J G G 2007; 34(12):1061-1071.
Qiu YX, Fu CX, Wu FJ. Analysis of population genetic structure and molecular identification of Changium smyrnioides and Chuanminshen violaceum with ISSR marker. Zhongguo Zhong Yao Za Zhi 2003; 28:598-603.
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References

Dey P, Kumar T. Pharmacological aspects of Nerium indicum Mill: A comprehensive review. Pharmacogn Rev 2014; 8(16):156-162.

Tahraoui A, El-Hilaly J, Israili ZH, Lyoussi B. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in South-easttern Morocco (Errachidia province). J Ethnopharmacol 2007; 110:105-117.

Lans C. Ethnomedicines used in Trinidad and Tobago for reproductive problems. J Ethnobiol Ethnomed 2007; 3:13-24.

Tagarelli G, Tagarelli A, Piro A. Folk medicine used to heal malaria in Calabria (southern Italy). J Ethnobiol Ethnomed 2010; 6:27-43.

Loveless MD, Hamrick JL. Ecological determinants of genetic structure in plant populations. Annu Rev Ecol Syst 1984; 15:65-95.

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990; 18:6531-6535.

Arnon DI. Copper enzyme polyphenoloxides in isolated chloroplast in Beta vulgaris. Plant physiol 1949; 24(1):1-15.

Kazemi M, Seyed Hajizadeh H. Assessment of genetic diversity of mints Iranian wild Mentha aquatic populations using RAPD markers. J Agr Tech 2012; 8(1):327-336.

Okon S, Surmacz-Magdziak A. The use of RAPD markers for detecting genetic similarity and molecular identification of chamomile (Chamomilla recutita (L.) Rausch.) genotypes. Herba Pol 2011; 57(1):38-47.

Shasany AK, Darokar MP, Dhawan S, Gupta AK, Gupta S, Shukla AK, et al. Use of RAPD and AFLP Markers to Identify Inter- and Intraspecific Hybrids of Mentha. J Hered 2005; 96(5):542-549.

RAPD 10mer KITS (KIT A) Sequences - Eurofins Genomics. http://www.eurofinsgenomics.eu.

Manuela M, Gouveia C. Genetic diversity in Hemileia vastatrix based on RAPD markers. Mycologia 2005; 97(2):396-404.

Powell W, Morgante M, Andre C, Vogel J, Tingey S, Rafalski A. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 1996; 2:225-238.

Rohlf FJ. On applications of geometric morphometrics to studies of ontogeny and phylogeny. Syst Biol 1998; 47:147– 158.

Peakall R, Smouse PE. GeneAlex 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update. Bioinformatics 2012; 28(19):2537-2539.

Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM. PopGene32, Microsoft Windows-based freeware for population genetic analysis, version 1.32. Molecular Biology and Biotechnology Center. University of Alberta, Canada. 2000.

Yuan Z, Yin Y, Qu J, Zhu L, Li Y, 2007. Population genetic diversity in Chinese pomegranate (Punica granatum L.) cultivars revealed by fluorescent-AFLP markers. J G G 2007; 34(12):1061-1071.

Qiu YX, Fu CX, Wu FJ. Analysis of population genetic structure and molecular identification of Changium smyrnioides and Chuanminshen violaceum with ISSR marker. Zhongguo Zhong Yao Za Zhi 2003; 28:598-603.

Author biographies is not available.