Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene

A. Z. Khan; I. Amad; S. Shaheen; K. Hussain; F. Hafeez; M. Farooq; H. Noor Ul Ayan

doi:10.14715/cmb/2017.63.10.9

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Vol. 63 No. 10: Issue 10

Issue Published : November 1, 2017

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Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene

https://doi.org/10.14715/cmb/2017.63.10.9

A. Z. Khan

Plant Research Group, Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan

I. Amad

Plant Research Group, Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan

S. Shaheen

Entomology Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan

K. Hussain

Plant Research Group, Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan

F. Hafeez

Department of Botany, Lahore College for Women University, Lahore, Pakistan

M. Farooq

Department of Botany, Lahore College for Women University, Lahore, Pakistan

H. Noor Ul Ayan

Plant Research Group, Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan

Corresponding Author(s) : K. Hussain

hussaink@gcuf.edu.pk

Cellular and Molecular Biology, Vol. 63 No. 10: Issue 10
Article Published : October 31, 2017

Abstract

Cotton dusky bug (Oxycarenus spp.) mostly attack on cash crops such as Gossypium, Cola and Hibiscus which affect the national economy therefore sustainable pest management is needed. Cytochrome c oxidase I (COI) gene is utilized as marker gene for DNA barcoding, genetic and ecological study of insects. In present study insect (cotton dusky bug) samples were collected from cotton fields in Faisalabad. COI gene was amplified from genomic DNA of bug and cloned into pTZ57R/T vector (Fermentas). The clone was sent to Macrogen (South Korea) for Sanger sequencing. The phylogenetic analysis and pairwise multiple sequence alignment showed that our cotton dusky bug grouped with two species of Oxycarenus genus and highest sequence identity was 91.1% with Oxycarenus hylinipennis. This is the first report of genetic barcode of Oxycarenus hylinipennis from cotton from Pakistan.

Keywords

Cotton dusky bug COI DNA barcoding Phylogenetic analysis Oxycarenus hyalinipennis.

Khan, A. Z., Amad, I., Shaheen, S., Hussain, K., Hafeez, F., Farooq, M., & Noor Ul Ayan, H. (2017). Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene. Cellular and Molecular Biology, 63(10), 59–63. https://doi.org/10.14715/cmb/2017.63.10.9

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References

Samy O. A revision of the African species of Oxycarenus (Hemiptera: Lygaeidae). Ecol Entomol 1969; 121:79-165.
Dimetry NZ. Studies on the Host Preference of the Cotton Seed Bug Oxycarenus hyalinipennis Costa (Lygaeidae: Hemiptera). J Appl Entomol 1971; 68:63-67.
Wasti SI. 2016. PAKISTAN ECONOMIC SURVEY 2015-16 In: Agriculture, editor. http://finance.gov.pk/survey_1516.html: Ministry of Finance, Government of Pakistan. p 41.
Khan M, Ahmed S. Toxicity of neem fruit extract and seed oil against Oxycarenus (Heteroptera) of cotton crop. Acta Biologica Cracoviensia/Zoologia 2000; 42:17-21.
Baranowski RM, Slater JA. 2005. Lygaeidae of the West Indies: Agricultural Experiment Station, Institute of Food and Agricultural Sciences, University of Florida.
Jaleel W, Saeed S, Naqqash MN, Zaka SM. Survey of Bt cotton in Punjab Pakistan related to the knowledge, perception and practices of farmers regarding insect pests. International Journal of Agriculture and Crop Sciences 2014; 7:10.
Sanghi AH, Aslam M, Khalid L. Efficacy of different insecticides against dusky cotton bug Oxycarenus hyalinipennis (Hemiptera: Lygaeidae) in ecological zone of Rahim yar khan. Int J Comp Res Biol Sci 2014; 1:49-54.
Hebert PD, Cywinska A, Ball SL. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences 2003; 270:313-321.
Ebach MC. Taxonomy and the DNA barcoding enterprise. Zootaxa 2011; 2742:67-68.
Hebert PD, Penton EH, Burns JM, Janzen DH, Hallwachs W. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc Natl Acad Sci U S A 2004; 101:14812-14817.
Löbl I, Leschen RA. Demography of coleopterists and their thoughts on DNA barcoding and the Phylocode, with commentary. Coleopt Bull 2005; 59:284-292.
Scheffer SJ, Lewis ML, Joshi RC. DNA barcoding applied to invasive leafminers (Diptera: Agromyzidae) in the Philippines. Ann Entomol Soc Am 2006; 99:204-210.
Ball SL, Hebert PD, Burian SK, Webb JM. Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. J North Am Benthol Soc 2005; 24:508-524.
Lee W, Kim H, Lim J, CHOI HR, Kim Y, KIM YSet al. Barcoding aphids (Hemiptera: Aphididae) of the Korean Peninsula: updating the global data set. Mol Ecol Resour 2011; 11:32-37.
Smith MA, Rodriguez JJ, Whitfield JB, Deans AR, Janzen DH, Hallwachs Wet al. Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections. Proc Natl Acad Sci 2008; 105:12359-12364.
Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PD. DNA barcodes distinguish species of tropical Lepidoptera. Proc Natl Acad Sci U S A 2006; 103:968-971.
Hebert PD, Ratnasingham S, de Waard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London B: Biological Sciences 2003; 270:S96-S99.
Hebert PD, Stoeckle MY, Zemlak TS, Francis CM. Identification of birds through DNA barcodes. PLoS Biol 2004; 2:e312.
Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PD. DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society of London B: Biological Sciences 2005; 360:1847-1857.
Dincćƒ V, Zakharov EV, Hebert PD, Vila R. Complete DNA barcode reference library for a country's butterfly fauna reveals high performance for temperate Europe. Proceedings of the Royal Society of London B: Biological Sciences 2011; 278:347-355.
Meier R, Zhang G, Ali F. The use of mean instead of smallest interspecific distances exaggerates the size of the "barcoding gap” and leads to misidentification. Syst Biol 2008; 57:809-813.
Hickerson MJ, Meyer CP, Moritz C. DNA barcoding will often fail to discover new animal species over broad parameter space. Syst Biol 2006; 55:729-739.
Bergsten J, Bilton DT, Fujisawa T, Elliott M, Monaghan MT, Balke Met al. The effect of geographical scale of sampling on DNA barcoding. Syst Biol 2012:sys037.
Hamilton CA, Hendrixson BE, Brewer MS, Bond JE. An evaluation of sampling effects on multiple DNA barcoding methods leads to an integrative approach for delimiting species: a case study of the North American tarantula genus Aphonopelma (Araneae, Mygalomorphae, Theraphosidae). Mol Phylogenet Evol 2014; 71:79-93.
Witt JD, Threloff DL, Hebert PD. DNA barcoding reveals extraordinary cryptic diversity in an amphipod genus: implications for desert spring conservation. Mol Ecol 2006; 15:3073-3082,Yassin A, Capy P, MADI"RAVAZZI L, Ogereau D, David JR. DNA barcode discovers two cryptic species and two geographical radiations in the invasive drosophilid Zaprionus indianus. Mol Ecol Resour 2008; 8:491-501.
Adamowicz SJ, Hebert PD, Marinone MC. Species diversity and endemism in the Daphnia of Argentina: a genetic investigation. Zool J Linn Soc 2004; 140:171-205.
Chen H, Rangasamy M, Tan SY, Wang H, Siegfried BD. Evaluation of five methods for total DNA extraction from western corn rootworm beetles. PLoS one 2010; 5:e11963.
Ashfaq M, Hebert PD, Mirza MS, Khan AM, Mansoor S, Shah GSet al. DNA barcoding of Bemisia tabaci complex (Hemiptera: Aleyrodidae) reveals southerly expansion of the dominant whitefly species on cotton in Pakistan. PloS one 2014; 9:e104485.
Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 2004; 101:11030-11035.

References

Samy O. A revision of the African species of Oxycarenus (Hemiptera: Lygaeidae). Ecol Entomol 1969; 121:79-165.

Dimetry NZ. Studies on the Host Preference of the Cotton Seed Bug Oxycarenus hyalinipennis Costa (Lygaeidae: Hemiptera). J Appl Entomol 1971; 68:63-67.

Wasti SI. 2016. PAKISTAN ECONOMIC SURVEY 2015-16 In: Agriculture, editor. http://finance.gov.pk/survey_1516.html: Ministry of Finance, Government of Pakistan. p 41.

Khan M, Ahmed S. Toxicity of neem fruit extract and seed oil against Oxycarenus (Heteroptera) of cotton crop. Acta Biologica Cracoviensia/Zoologia 2000; 42:17-21.

Baranowski RM, Slater JA. 2005. Lygaeidae of the West Indies: Agricultural Experiment Station, Institute of Food and Agricultural Sciences, University of Florida.

Jaleel W, Saeed S, Naqqash MN, Zaka SM. Survey of Bt cotton in Punjab Pakistan related to the knowledge, perception and practices of farmers regarding insect pests. International Journal of Agriculture and Crop Sciences 2014; 7:10.

Sanghi AH, Aslam M, Khalid L. Efficacy of different insecticides against dusky cotton bug Oxycarenus hyalinipennis (Hemiptera: Lygaeidae) in ecological zone of Rahim yar khan. Int J Comp Res Biol Sci 2014; 1:49-54.

Hebert PD, Cywinska A, Ball SL. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences 2003; 270:313-321.

Ebach MC. Taxonomy and the DNA barcoding enterprise. Zootaxa 2011; 2742:67-68.

Hebert PD, Penton EH, Burns JM, Janzen DH, Hallwachs W. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc Natl Acad Sci U S A 2004; 101:14812-14817.

Löbl I, Leschen RA. Demography of coleopterists and their thoughts on DNA barcoding and the Phylocode, with commentary. Coleopt Bull 2005; 59:284-292.

Scheffer SJ, Lewis ML, Joshi RC. DNA barcoding applied to invasive leafminers (Diptera: Agromyzidae) in the Philippines. Ann Entomol Soc Am 2006; 99:204-210.

Ball SL, Hebert PD, Burian SK, Webb JM. Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. J North Am Benthol Soc 2005; 24:508-524.

Lee W, Kim H, Lim J, CHOI HR, Kim Y, KIM YSet al. Barcoding aphids (Hemiptera: Aphididae) of the Korean Peninsula: updating the global data set. Mol Ecol Resour 2011; 11:32-37.

Smith MA, Rodriguez JJ, Whitfield JB, Deans AR, Janzen DH, Hallwachs Wet al. Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections. Proc Natl Acad Sci 2008; 105:12359-12364.

Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PD. DNA barcodes distinguish species of tropical Lepidoptera. Proc Natl Acad Sci U S A 2006; 103:968-971.

Hebert PD, Ratnasingham S, de Waard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London B: Biological Sciences 2003; 270:S96-S99.

Hebert PD, Stoeckle MY, Zemlak TS, Francis CM. Identification of birds through DNA barcodes. PLoS Biol 2004; 2:e312.

Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PD. DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society of London B: Biological Sciences 2005; 360:1847-1857.

Dincćƒ V, Zakharov EV, Hebert PD, Vila R. Complete DNA barcode reference library for a country's butterfly fauna reveals high performance for temperate Europe. Proceedings of the Royal Society of London B: Biological Sciences 2011; 278:347-355.

Meier R, Zhang G, Ali F. The use of mean instead of smallest interspecific distances exaggerates the size of the "barcoding gap” and leads to misidentification. Syst Biol 2008; 57:809-813.

Hickerson MJ, Meyer CP, Moritz C. DNA barcoding will often fail to discover new animal species over broad parameter space. Syst Biol 2006; 55:729-739.

Bergsten J, Bilton DT, Fujisawa T, Elliott M, Monaghan MT, Balke Met al. The effect of geographical scale of sampling on DNA barcoding. Syst Biol 2012:sys037.

Hamilton CA, Hendrixson BE, Brewer MS, Bond JE. An evaluation of sampling effects on multiple DNA barcoding methods leads to an integrative approach for delimiting species: a case study of the North American tarantula genus Aphonopelma (Araneae, Mygalomorphae, Theraphosidae). Mol Phylogenet Evol 2014; 71:79-93.

Witt JD, Threloff DL, Hebert PD. DNA barcoding reveals extraordinary cryptic diversity in an amphipod genus: implications for desert spring conservation. Mol Ecol 2006; 15:3073-3082,Yassin A, Capy P, MADI"RAVAZZI L, Ogereau D, David JR. DNA barcode discovers two cryptic species and two geographical radiations in the invasive drosophilid Zaprionus indianus. Mol Ecol Resour 2008; 8:491-501.

Adamowicz SJ, Hebert PD, Marinone MC. Species diversity and endemism in the Daphnia of Argentina: a genetic investigation. Zool J Linn Soc 2004; 140:171-205.

Chen H, Rangasamy M, Tan SY, Wang H, Siegfried BD. Evaluation of five methods for total DNA extraction from western corn rootworm beetles. PLoS one 2010; 5:e11963.

Ashfaq M, Hebert PD, Mirza MS, Khan AM, Mansoor S, Shah GSet al. DNA barcoding of Bemisia tabaci complex (Hemiptera: Aleyrodidae) reveals southerly expansion of the dominant whitefly species on cotton in Pakistan. PloS one 2014; 9:e104485.

Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 2004; 101:11030-11035.

Author biographies is not available.