Effects of different levels of dietary Citrus Limon essential oil on some blood parameters and antioxidant status in Afshari Ewes

Ali Mojtahedin; Jamal Seifdavati; Reza Seyedsharifi

doi:10.14715/cmb/2018.64.1.9

Issue

Vol. 64 No. 1: Issue 1

Issue Published : February 7, 2018

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Effects of different levels of dietary Citrus Limon essential oil on some blood parameters and antioxidant status in Afshari Ewes

https://doi.org/10.14715/cmb/2018.64.1.9

Ali Mojtahedin

Department of Physiology, Moghan Faculty of Agriculture & Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

Jamal Seifdavati

Department of of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran

Reza Seyedsharifi

Department of of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran

Corresponding Author(s) : Ali Mojtahedin

mojtahedin_dvsc@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 1: Issue 1
Article Published : January 31, 2018

Abstract

Citrus Limon Oil (CLO) is known as antioxidant resource and contains limonoids, flavonoids and phenolic compounds. This study was conducted to investigate the effects of different levels of CLO on blood parameters and antioxidant status in Afshari ewes. Six adults Afshari ewes (3-4 years old and 51±5 kg) were randomly allocated to 3í—3 Latin square design with three diets in 21 days period. Dietary treatments included:1) control diet, 2) control diet with 200 mg/day CLO, and 3) control diet with 400 mg/day CLO. To evaluate the antioxidant effect of the CLO, sustainable elimination of free radicals by DPPH and ABTS methods were used. The antioxidant activity of essential oils in DPPH method at doses of 32.5, 45, 130, 260 and 520 mg/ml were 9, 16, 31, 49 and 89%, respectively. Also, antioxidant activity of essential oils in ABTS method at doses of 32.5, 45, 130, 260 and 520 mg/ml were 49, 73, 81, 89 and 95%, respectively. CLO treatments did not affect glucose, blood urea nitrogen, albumin, total protein, low density lipoprotein, while improved the concentration of high-density lipoprotein (P>0.01). Results showed that supplementation with CLO significantly decreased (P<0.01) cholesterol, triglycerides and very low density lipoprotein concentrations compared with control. There was no significant difference in analyzed blood bio-chemicals and serum enzymes level between different antioxidant activity methods and groups, suggesting general well-being of ewes. These results suggest that, CLO supplementation had a positive impact on blood traits and antioxidant status of the Afshari ewes.

Keywords

Afshari ewe Antioxidant status Biochemical profile Blood parameters Citrus Limon Oil.

Mojtahedin, A., Seifdavati, J., & Seyedsharifi, R. (2018). Effects of different levels of dietary Citrus Limon essential oil on some blood parameters and antioxidant status in Afshari Ewes. Cellular and Molecular Biology, 64(1), 47–51. https://doi.org/10.14715/cmb/2018.64.1.9

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References

Alhidary IA, Abdelrahman MM, Harron RM. Effects of a long acting trace mineral rumen bolus supplement on growth performance, metabolic profiles, and trace mineral status of growing camels. Trop Anim Health Prod, 2016; 48(4): 763–768.
Shahabi H, Chashnidel Y. The effect of canola oil and oregano essential oil on performance, blood parameters and chemical carcass compositions of Dalagh fattening lambs. J Rum Res, 2014; 2(1): 33-50. (In Persian).
Sretenovic LJ, Aleksic S, Petrovic MP, Miscevic B. Nutritional factors influencing improvement of milk and meat quality as well as productive and reproductive parameters of cattle. Biotechnol Anim Husb, 2007; 23(5-6): 217–226.
Mokbel MS, Watanabe Y, Hashinaga F, Suganuma T. Purification of antioxidant and antimicrobial substance of Ethyl acetate from Buntan (Citrus grandisbeck) Fruit peel. Pak J Bio Sci, 2006; 9(1): 1445-1450.
Askar A, Treptow H. Nivea Produkte Bei der Verarbeitung Tropischer Früchte. Industrielle Obst-und Gemüseverwertung, 1998; 83: 7-13.
Chung MJ, Kang AY, Park SO, Park KW, Jun HJ, Lee SJ. The effect of essential oils of dietary wormwood (Artemisia princes), with and without added vitamin E, on oxidative stress and some genes involved in cholesterol metabolism. Food Chem Toxicol, 2007; 45(8): 1400–1409.
Chung MJ, Park KW, Kim KH, Kim CT, Baek JP, Bang KH, et al. Asian plantain (Plantago asiatica) essential oils suppress 3-hydroxy-3-methylglutaryl- co-enzyme A reductase expression in vitro and in vivo and show hypocholesterolaemic properties in mice. Br J Nutr, 2008; 99(1): 67–75.
Takahama U. Inhibition of lipoxygenase dependent lipid peroxidation by quercetin: Mechanism of antioxidative function. Phytochemistry, 2008; 24(7): 1443-1446.
Tzung-Hsun T, Tsung-Hsein T, You-Chia C, ChiWei L, Po-jung T. In vitro Antimicrobial activities against cariogenic streptococci and their antioxidant capacities: A comparative study of green tea versus different herbs. Food Chem, 2008; 110(4): 859-864.
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm Wissu Technol, 1995; 28(1): 25-30.
Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res, 2000; 14(5): 323-328.
SAS Institute, ‘SAS/STATuser's guide. Statistics. Version 9.1, (SAS Institute Inc. Cary, NC), 2001.
Ghasemi K, Ghasemi Y, Ebrahimzadeh MA. Antioxidant activity, phenol and flavonoid contents of 13 Citrus species peels and tissues. Pak J Pharm Sci, 2009; 22(3): 277-281.
Misharina TA, Samusenko AL. Antioxidant properties of essential oils from lemon, grapefruit, coriander, clove and their mixtures. Prikl Biokhim Mikrobiol, 2008; 44(4): 438-442. (In Russian).
Sharafi SM, Rasooli I, Allahghadri T, JalaliNadoushan MR, Rezaei MB. Antimicrobial, antioxidant, hematologic and cytotoxic properties of Citrus limon L. essential oil. Iranian J Medi Arom Plants, 2010; 26(3) 423-437.
Campelo LM, Gonçalves FC, Feitosa CM, de Freitas RM. Antioxidant activity of Citrus limon essential oil in mouse hippocampus. Pharm Bio, 2011; 49(7): 709-15.
Nobakht A, AmiriDashatan B. Effect of different levels of lemon pulp (Citrus aurantifulia) on performance, carcass and blood parameters of meat type turkeys. Anim Prod Res, 2014; 3(2): 9-17. (In Persian).
Akbarian A1, Golian A, Kermanshahi H, DeSmet S, Michiels J. Antioxidant enzyme activities, plasma hormone levels and serum metabolites of finishing broiler chickens reared under high ambient temperature and fed lemon and orange peel extracts and Curcuma xanthorrhiza essential oil. J Anim Physiol Anim Nutr (Berl), 2015; 99(1): 150-162.
Yaghmae P, Parivar K, Haftsavar M, Zaerbinan F, Shahsavari S. Study of the effect of Lemon peel essential oil on blood lipid levels and differential leukocyte count. Scientific J Kurdistan Uni Med Sci, 2009; 14(1): 55-64. (In Persian)
Edris AE. Pharmaceutical and Therapeutic Potentials of Essential Oils and Their Individual Volatile Constituents: A Review. Phytother Res, 2007; 21(4): 308-323.
Sarica S, Toptas S. Effects of dietary oleuropein supplementation on growth performance, serum lipid concentrations and lipid oxidation of Japanese quails. J Anim Physiol Anim Nutr (Berl) , 2014; 98(6): 1176-86.
Garjani A, Fathiazad F, Zakheri A, Akbari NA. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status and vascular function in hypercholesterolemic rats. J Ethnopharmacol, 2009; 126(1): 123-135.
Fukuchi Y, Hiramitsu M, Okada M, Hayashi S, Nabeno Y, Osawa T, et al. Lemon polyphenols suppress diet-induced obesity by up regulation of mRNA levels of the enzymes involved in Î²-Oxidation in mouse white adipose tissue. J Clin Biochem Nutr, 2008; 43(3): 201–209.

References

Alhidary IA, Abdelrahman MM, Harron RM. Effects of a long acting trace mineral rumen bolus supplement on growth performance, metabolic profiles, and trace mineral status of growing camels. Trop Anim Health Prod, 2016; 48(4): 763–768.

Shahabi H, Chashnidel Y. The effect of canola oil and oregano essential oil on performance, blood parameters and chemical carcass compositions of Dalagh fattening lambs. J Rum Res, 2014; 2(1): 33-50. (In Persian).

Sretenovic LJ, Aleksic S, Petrovic MP, Miscevic B. Nutritional factors influencing improvement of milk and meat quality as well as productive and reproductive parameters of cattle. Biotechnol Anim Husb, 2007; 23(5-6): 217–226.

Mokbel MS, Watanabe Y, Hashinaga F, Suganuma T. Purification of antioxidant and antimicrobial substance of Ethyl acetate from Buntan (Citrus grandisbeck) Fruit peel. Pak J Bio Sci, 2006; 9(1): 1445-1450.

Askar A, Treptow H. Nivea Produkte Bei der Verarbeitung Tropischer Früchte. Industrielle Obst-und Gemüseverwertung, 1998; 83: 7-13.

Chung MJ, Kang AY, Park SO, Park KW, Jun HJ, Lee SJ. The effect of essential oils of dietary wormwood (Artemisia princes), with and without added vitamin E, on oxidative stress and some genes involved in cholesterol metabolism. Food Chem Toxicol, 2007; 45(8): 1400–1409.

Chung MJ, Park KW, Kim KH, Kim CT, Baek JP, Bang KH, et al. Asian plantain (Plantago asiatica) essential oils suppress 3-hydroxy-3-methylglutaryl- co-enzyme A reductase expression in vitro and in vivo and show hypocholesterolaemic properties in mice. Br J Nutr, 2008; 99(1): 67–75.

Takahama U. Inhibition of lipoxygenase dependent lipid peroxidation by quercetin: Mechanism of antioxidative function. Phytochemistry, 2008; 24(7): 1443-1446.

Tzung-Hsun T, Tsung-Hsein T, You-Chia C, ChiWei L, Po-jung T. In vitro Antimicrobial activities against cariogenic streptococci and their antioxidant capacities: A comparative study of green tea versus different herbs. Food Chem, 2008; 110(4): 859-864.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm Wissu Technol, 1995; 28(1): 25-30.

Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res, 2000; 14(5): 323-328.

SAS Institute, ‘SAS/STATuser's guide. Statistics. Version 9.1, (SAS Institute Inc. Cary, NC), 2001.

Ghasemi K, Ghasemi Y, Ebrahimzadeh MA. Antioxidant activity, phenol and flavonoid contents of 13 Citrus species peels and tissues. Pak J Pharm Sci, 2009; 22(3): 277-281.

Misharina TA, Samusenko AL. Antioxidant properties of essential oils from lemon, grapefruit, coriander, clove and their mixtures. Prikl Biokhim Mikrobiol, 2008; 44(4): 438-442. (In Russian).

Sharafi SM, Rasooli I, Allahghadri T, JalaliNadoushan MR, Rezaei MB. Antimicrobial, antioxidant, hematologic and cytotoxic properties of Citrus limon L. essential oil. Iranian J Medi Arom Plants, 2010; 26(3) 423-437.

Campelo LM, Gonçalves FC, Feitosa CM, de Freitas RM. Antioxidant activity of Citrus limon essential oil in mouse hippocampus. Pharm Bio, 2011; 49(7): 709-15.

Nobakht A, AmiriDashatan B. Effect of different levels of lemon pulp (Citrus aurantifulia) on performance, carcass and blood parameters of meat type turkeys. Anim Prod Res, 2014; 3(2): 9-17. (In Persian).

Akbarian A1, Golian A, Kermanshahi H, DeSmet S, Michiels J. Antioxidant enzyme activities, plasma hormone levels and serum metabolites of finishing broiler chickens reared under high ambient temperature and fed lemon and orange peel extracts and Curcuma xanthorrhiza essential oil. J Anim Physiol Anim Nutr (Berl), 2015; 99(1): 150-162.

Yaghmae P, Parivar K, Haftsavar M, Zaerbinan F, Shahsavari S. Study of the effect of Lemon peel essential oil on blood lipid levels and differential leukocyte count. Scientific J Kurdistan Uni Med Sci, 2009; 14(1): 55-64. (In Persian)

Edris AE. Pharmaceutical and Therapeutic Potentials of Essential Oils and Their Individual Volatile Constituents: A Review. Phytother Res, 2007; 21(4): 308-323.

Sarica S, Toptas S. Effects of dietary oleuropein supplementation on growth performance, serum lipid concentrations and lipid oxidation of Japanese quails. J Anim Physiol Anim Nutr (Berl) , 2014; 98(6): 1176-86.

Garjani A, Fathiazad F, Zakheri A, Akbari NA. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status and vascular function in hypercholesterolemic rats. J Ethnopharmacol, 2009; 126(1): 123-135.

Fukuchi Y, Hiramitsu M, Okada M, Hayashi S, Nabeno Y, Osawa T, et al. Lemon polyphenols suppress diet-induced obesity by up regulation of mRNA levels of the enzymes involved in Î²-Oxidation in mouse white adipose tissue. J Clin Biochem Nutr, 2008; 43(3): 201–209.