The relationship between gastric cancer and Helicobacter pylori cytotoxin-related gene A genotypes

Huai Kun Ni; Lian Ming liao; Ruo Lei Huang; Wenjuan Zhou

doi:10.14715/cmb/2020.66.7.1

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Vol. 66 No. 7: Issue 7

Issue Published : November 9, 2020

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The relationship between gastric cancer and Helicobacter pylori cytotoxin-related gene A genotypes

https://doi.org/10.14715/cmb/2020.66.7.1

Huai Kun Ni

General surgery department, Fujian Provincial Hospital South Branch, Fuzhou, China

Lian Ming liao

Central Lab, Union Hospital affiliated to Fujian Medical University, Fuzhou, China

Ruo Lei Huang

General surgery department, Fujian Provincial Hospital South Branch, Fuzhou, China

Wenjuan Zhou

Clinical Laboratory, Union Hospital affiliated to Fujian Medical University, Fuzhou, China

Corresponding Author(s) : Huai Kun Ni

179008473@qq.com

Cellular and Molecular Biology, Vol. 66 No. 7: Issue 7
Article Published : October 31, 2020

Abstract

Gastric cancer has been known as the third leading cause of cancer-related death in the world. It is when cancer cells form on the lining of the stomach. Early symptoms include heartburn, upper abdominal pain, nausea, and loss of appetite. Helicobacter pylori is the most common microscopic creature that has infected humans worldwide. More than half of the world's population is infected with the bacterium. It is the main cause of diseases such as stomach ulcers and stomach and intestinal disorders. H. pylori infection is related to gastric adenocarcinoma and cagA genotype is believed to be related to cancer development. cytotoxin-associated gene A (CagA) is a 120–145kDa protein encoded on the 40kb cag pathogenicity island (PAI). This study investigates the association between cagA H. pylori genotypes and gastric cancer. For this purpose, 65 stomach biopsies of the gastric cancer patients and 100 saliva samples were collected from healthy and H. pylori-infected individuals. Then genomic DNA was purified and Polymerase Chain Reaction (PCR) was performed for the studied gene using specific primers. The presence of H. pylori was investigated by PCR and a pair of specific primers for a protected region in the bacterium glmM gene. Then cagA+ and cagA- genotypes frequencies were determined in H. pylori-infected cases. Statistical analysis showed that there were significant differences between healthy and diseased ones for genotypes cagA+ and cagA-. Then the cagA+ can be a risk factor genotype for gastric cancer.

Keywords

cagA genotyping Gastric cancer Helicobacter pylori

Ni, H. K., liao, L. M., Huang, R. L., & Zhou, W. (2020). The relationship between gastric cancer and Helicobacter pylori cytotoxin-related gene A genotypes. Cellular and Molecular Biology, 66(7), 1–4. https://doi.org/10.14715/cmb/2020.66.7.1

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References

Ruddon R.W. Cancer biology (4th ed.), Oxford: Oxford University Press, 2007, pp. 223.
Orditura M, Galizia G, Sforza V, Gambardella V, Fabozzi A, Laterza MM and et al. Treatment of gastric cance. World Journal of Gastroenterology 2014; 20 (7): 1635–49.
Khleif M, Roland T, Samir N. Handbook of cancer chemotherapy (8th ed.), Philadelphia: Wolter Kluwer, 2011, pp. 127.
Chang A. H, Parsonnet J. Role of Bacteria in Oncogenesis. Clinical Microbiology Reviews 2010; 23 (4): 837–857.
Olson JW, Maier RJ. Molecular hydrogen as an energy source for Helicobacter pylori. Science 2002; 298 (5599): 1788–1790.
Stark RM, Gerwig GJ, Pitman RS, Potts LF, Williams NA, Greenman J and et al. Biofilm formation by Helicobacter pylori. Lett Appl Microbiol 1999; 28 (2): 121–126.
Chan WY, Hui PK, Leung KM, Chow J, Kwok F, Ng CS. Coccoid forms of Helicobacter pylori in the human stomach. Am J Clin Pathol 1994; 102 (4): 503–507.
Hatakeyama M, Higashi H. Helicobacter pylori CagA: a new paradigm for bacterial carcinogenesis. Cancer Science 2005; 96: 835–843.
Lax, A. Bacterial toxins and cancer ” a case to answer? Nature Reviews Microbiology 2005; 3: 343–9.
Yamaoka Y. Mechanisms of disease: Helicobacter pylori virulence factors. Nat Rev Gastroenterol Hepatol 2010; 7 (11): 629–41.
Moradi M.T, Yari K, Khodarahmi R. A novel, efficient, fast and inexpensive DNA extraction protocol from whole blood appli¬cable for studying drug-DNA interaction. J. Rep. Pharm. Sci. 2014; 3(1): 80-84.
Aidar M. A simple and cos,t-effective protocol for DNA isolation from buccal epithelial cells. Braz. Dent. J. 2007; 18(2): 148-152.
Moges F, Kassu A, Mengistu G, Adugna S, Andualem B, Nishikawa T and et al. Seroprevalence of Helicobacter pylori in dyspeptic patients and its relationship with HIV infection, ABO blood groups and life style in a university hospital, Northwest Ethiopia.World J. Gastroen 2006; 12(12): 1957-1961.
Espinoza M.G.C, Vazquez R.G, Mendez I.M, Vargas C.R, Cerezo S.G. Detection of the glmM Gene in Helicobacter pyloriIsolates with a Novel Primer by PCR. Journal of Clinical Microbiology 2011; 49(4):1650-1652
Blaser M.J, Perez-Perez G.I, Kleanthous H, Cover T.L, Peek R.M, Chyou P.H and et al. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Research 1995; 55: 2111-2115.
Peek J, Blaser M. Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nature 2002; 2: 28–37.
Saribasak H, Salih B, Yamaoka Y, Sander E. Analysis of Helicobacter pylori Genotypes and Correlation with Clinical Outcome in Turkey. Journal of Clinical Microbiology 2004; 42: 1648-1651.
Miehlke S, Kirsch C, Agha-Amiri K, Gunther T, Lehn N, Malfertheiner P, Stolte M, Bayerd E. The Helicobacter pylori vacA s1, m1 genotype and cagA is associated with gastric carcinoma in Germany. International Journal of Cancer 2000; 87: 322-327.
Talebkhan Y, Mohammadi M, Mohagheghi M. A, Vaziri H.R, Eshagh Hosseini M, Mohajerani N and et al. cagA gene and protein status among Iranian Helicobacter pylori strains. Digestive Diseases and Sciences 2008; 53(4): 925-32.
Torres J, Pérez-Pérez G, Leal-Herrera Y, Muñoz O. Infection with CagA+ Helicobacter pylori strains as a possible predictor of risk in the development of gastric adenocarcinoma in Mexico; International Journal of Cancer 1998; 78: 298–300.

References

Ruddon R.W. Cancer biology (4th ed.), Oxford: Oxford University Press, 2007, pp. 223.

Orditura M, Galizia G, Sforza V, Gambardella V, Fabozzi A, Laterza MM and et al. Treatment of gastric cance. World Journal of Gastroenterology 2014; 20 (7): 1635–49.

Khleif M, Roland T, Samir N. Handbook of cancer chemotherapy (8th ed.), Philadelphia: Wolter Kluwer, 2011, pp. 127.

Chang A. H, Parsonnet J. Role of Bacteria in Oncogenesis. Clinical Microbiology Reviews 2010; 23 (4): 837–857.

Olson JW, Maier RJ. Molecular hydrogen as an energy source for Helicobacter pylori. Science 2002; 298 (5599): 1788–1790.

Stark RM, Gerwig GJ, Pitman RS, Potts LF, Williams NA, Greenman J and et al. Biofilm formation by Helicobacter pylori. Lett Appl Microbiol 1999; 28 (2): 121–126.

Chan WY, Hui PK, Leung KM, Chow J, Kwok F, Ng CS. Coccoid forms of Helicobacter pylori in the human stomach. Am J Clin Pathol 1994; 102 (4): 503–507.

Hatakeyama M, Higashi H. Helicobacter pylori CagA: a new paradigm for bacterial carcinogenesis. Cancer Science 2005; 96: 835–843.

Lax, A. Bacterial toxins and cancer ” a case to answer? Nature Reviews Microbiology 2005; 3: 343–9.

Yamaoka Y. Mechanisms of disease: Helicobacter pylori virulence factors. Nat Rev Gastroenterol Hepatol 2010; 7 (11): 629–41.

Moradi M.T, Yari K, Khodarahmi R. A novel, efficient, fast and inexpensive DNA extraction protocol from whole blood appli¬cable for studying drug-DNA interaction. J. Rep. Pharm. Sci. 2014; 3(1): 80-84.

Aidar M. A simple and cos,t-effective protocol for DNA isolation from buccal epithelial cells. Braz. Dent. J. 2007; 18(2): 148-152.

Moges F, Kassu A, Mengistu G, Adugna S, Andualem B, Nishikawa T and et al. Seroprevalence of Helicobacter pylori in dyspeptic patients and its relationship with HIV infection, ABO blood groups and life style in a university hospital, Northwest Ethiopia.World J. Gastroen 2006; 12(12): 1957-1961.

Espinoza M.G.C, Vazquez R.G, Mendez I.M, Vargas C.R, Cerezo S.G. Detection of the glmM Gene in Helicobacter pyloriIsolates with a Novel Primer by PCR. Journal of Clinical Microbiology 2011; 49(4):1650-1652

Blaser M.J, Perez-Perez G.I, Kleanthous H, Cover T.L, Peek R.M, Chyou P.H and et al. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Research 1995; 55: 2111-2115.

Peek J, Blaser M. Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nature 2002; 2: 28–37.

Saribasak H, Salih B, Yamaoka Y, Sander E. Analysis of Helicobacter pylori Genotypes and Correlation with Clinical Outcome in Turkey. Journal of Clinical Microbiology 2004; 42: 1648-1651.

Miehlke S, Kirsch C, Agha-Amiri K, Gunther T, Lehn N, Malfertheiner P, Stolte M, Bayerd E. The Helicobacter pylori vacA s1, m1 genotype and cagA is associated with gastric carcinoma in Germany. International Journal of Cancer 2000; 87: 322-327.

Talebkhan Y, Mohammadi M, Mohagheghi M. A, Vaziri H.R, Eshagh Hosseini M, Mohajerani N and et al. cagA gene and protein status among Iranian Helicobacter pylori strains. Digestive Diseases and Sciences 2008; 53(4): 925-32.

Torres J, Pérez-Pérez G, Leal-Herrera Y, Muñoz O. Infection with CagA+ Helicobacter pylori strains as a possible predictor of risk in the development of gastric adenocarcinoma in Mexico; International Journal of Cancer 1998; 78: 298–300.

Author biographies is not available.