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Vol. 64 No. 3: Issue 3

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Cytotoxic effects of new-generation bulk-fill composites on human dental pulp stem cells

https://doi.org/10.14715/cmb/2018.64.3.11
Hakan Kamalak
Department of Restorative Dentistry, Faculty of Dentistry, Firat University, Elazig, Turkey
Aliye Kamalak
Department of Endodontics, Faculty of Dentistry, Firat University, Elazig, Turkey
Ali Taghizadehghalehjoughi
Medical Genetics, Faculty of Medicine, Atatürk University, Erzurum, Turkey

Corresponding Author(s) : Hakan Kamalak

hakankamalak@hotmail.com

Cellular and Molecular Biology, Vol. 64 No. 3: Issue 3

Abstract

This study was performed to evaluate possible DNA damage in cells of human origin exposed to dental composites in vitro using a cytotoxic assay. Five bulk-fill composites were filled in molds and irradiated for 20 s. DPSCs were inoculated into 24-well plates. After the insert membrane was inserted and composites were added and the experiment was continued for 24/72 hours. In order to investigate the effects of the materials on DPSCs; its effect on apoptosis-regulating Bcl-2 gene, Human Beta-Defensins (HBDs 1-2) gene, Interleukin 6, 8, 10 expression level was examined. Also in order to check the cellular viability and stress factors; MTT assay, Total Antioxidant and Oxidant Status kits were used. At both irradiation times, all composites significantly affected analyses parameters used in primary DNA damage assessment or induced significant formation of cellular death. Cytotoxicity was detected in TE<SS<FBF<XB<VBF groups at 24 hour, and after 72 hour this sequence has changed.

Keywords

Cytotoxicity Apoptosis Human beta defensins Stem cells.
Kamalak, H., Kamalak, A., & Taghizadehghalehjoughi, A. (2018). Cytotoxic effects of new-generation bulk-fill composites on human dental pulp stem cells. Cellular and Molecular Biology, 64(3), 62–71. https://doi.org/10.14715/cmb/2018.64.3.11
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References
  1. Six N, Lasfargues JJ, Goldberg M. In vivo study of the pulp reaction to Fuji IX, a glass ionomer cement. J Dent. 2000; 28: 413-22.
  2. Modena KC, Casas-Apayco LC, Atta MT et al. Cytotoxicity and biocompatibility of direct and indirect pulp capping materials. J Appl Oral Sci. 2009; 17: 544-54.
  3. Brannstrom M, Vojinovic O. Response of the dental pulp to invasion of bacteria around three filling materials. ASDC J Dent Child. 1976; 43: 83-9.
  4. Cox CF, Keall CL, Keall HJ, Ostro E, Bergenholtz G. Biocompatibility of surface-sealed dental materials against exposed pulps. J Prosthet Dent. 1987; 57: 1-8.
  5. Grieve AR, Alani A, Saunders WP. The effects on the dental pulp of a composite resin and two dentine bonding agents and associated bacterial microleakage. Int Endod J. 1991; 24: 108-18.
  6. Hanks CT, Wataha JC, Sun Z. In vitro models of biocompatibility: a review. Dent Mater. 1996; 12: 186-93.
  7. Murray PE, Garcia Godoy C, Garcia Godoy F. How is the biocompatibilty of dental biomaterials evaluated? Med Oral Patol Oral Cir Bucal. 2007; 12: E258-66.
  8. Wataha JC. Principles of biocompatibility for dental practitioners. J Prosthet Dent. 2001; 86: 203-9.
  9. de Souza Costa CA, Teixeira HM, Lopes do Nascimento AB, Hebling J. Biocompatibility of resin-based dental materials applied as liners in deep cavities prepared in human teeth. J Biomed Mater Res B Appl Biomater. 2007; 81: 175-84.
  10. Schweikl H, Schmalz G, Rackebrandt K. The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells. Mutat Res. 1998; 415: 119-30.
  11. Schweikl H, Schmalz G. Triethylene glycol dimethacrylate induces large deletions in the hprt gene of V79 cells. Mutat Res. 1999; 438: 71-8.
  12. Schweikl H, Schmalz G, Spruss T. The induction of micronuclei in vitro by unpolymerized resin monomers. J Dent Res. 2001; 80: 1615-20.
  13. Kleinsasser NH, Schmid K, Sassen AW et al. Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay. Biomaterials. 2006; 27: 1762-70.
  14. Harris W. Cancer and the vegetarian diet. The Scientific Basis of Vegetarianism. 1999.
  15. Thompson EB. Apoptosis and steroid hormones. Mol Endocrinol. 1994; 8: 665-73.
  16. Porter EM, Liu L, Oren A, Anton PA, Ganz T. Localization of human intestinal defensin 5 in Paneth cell granules. Infect Immun. 1997; 65: 2389-95.
  17. Schneider JJ, Unholzer A, Schaller M, Schafer-Korting M, Korting HC. Human defensins. J Mol Med (Berl). 2005; 83: 587-95.
  18. Diamond G, Russell JP, Bevins CL. Inducible expression of an antibiotic peptide gene in lipopolysaccharide-challenged tracheal epithelial cells. Proc Natl Acad Sci U S A. 1996; 93: 5156-60.
  19. Dommisch H, Winter J, Acil Y, Dunsche A, Tiemann M, Jepsen S. Human beta-defensin (hBD-1, -2) expression in dental pulp. Oral Microbiol Immunol. 2005; 20: 163-6.
  20. Dommisch H, Winter J, Willebrand C, Eberhard J, Jepsen S. Immune regulatory functions of human beta-defensin-2 in odontoblast-like cells. Int Endod J. 2007; 40: 300-7.
  21. Shiba H, Mouri Y, Komatsuzawa H et al. Macrophage inflammatory protein-3α and β-defensin-2 stimulate dentin sialophosphoprotein gene expression in human pulp cells. Biochemical and biophysical research communications. 2003; 306: 867-71.
  22. Feucht EC, DeSanti CL, Weinberg A. Selective induction of human beta-defensin mRNAs by Actinobacillus actinomycetemcomitans in primary and immortalized oral epithelial cells. Oral Microbiol Immunol. 2003; 18: 359-63.
  23. Krisanaprakornkit S, Kimball JR, Weinberg A, Darveau RP, Bainbridge BW, Dale BA. Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. Infect Immun. 2000; 68: 2907-15.
  24. Mueller CK, Lee SY, Schultze-Mosgau S. Characterization of interfacial reactions between connective tissue and allogenous implants used for subdermal soft tissue augmentation. Int J Oral Maxillofac Surg. 2009; 38: 1194-200.
  25. Imazato S, McCabe JF, Tarumi H, Ehara A, Ebisu S. Degree of conversion of composites measured by DTA and FTIR. Dent Mater. 2001; 17: 178-83.
  26. Hume WR, Gerzina TM. Bioavailability of components of resin-based materials which are applied to teeth. Crit Rev Oral Biol Med. 1996; 7: 172-9.
  27. Geurtsen W, Spahl W, Leyhausen G. Variability of cytotoxicity and leaching of substances from four light-curing pit and fissure sealants. J Biomed Mater Res. 1999; 44: 73-7.
  28. Geurtsen W, Spahl W, Leyhausen G. Residual monomer/additive release and variability in cytotoxicity of light-curing glass-ionomer cements and compomers. J Dent Res. 1998; 77: 2012-9.
  29. Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res. 1991; 70: 1450-5.
  30. Ferracane JL, Condon JR. Rate of elution of leachable components from composite. Dent Mater. 1990; 6: 282-7.
  31. Melo MA, Moyses MR, Santos SG, Alcantara CE, Ribeiro JC. Effects of different surface treatments and accelerated artificial aging on the bond strength of composite resin repairs. Braz Oral Res. 2011; 25: 485-91.
  32. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000; 97: 13625-30.
  33. Krebsbach PH, Robey PG. Dental and skeletal stem cells: potential cellular therapeutics for craniofacial regeneration. J Dent Educ. 2002; 66: 766-73.
  34. Todorovic D, Petrovic I, Todorovic M, Cuttone G, Ristic-Fira A. Early effects of gamma rays and protons on human melanoma cell viability and morphology. J Microsc. 2008; 232: 517-21.
  35. Wiegand A, Buchholz K, Werner C, Attin T. In vitro cytotoxicity of different desensitizers under simulated pulpal flow conditions. J Adhes Dent. 2008; 10: 227-32.
  36. Trichaiyapon V, Torrungruang K, Panitvisai P. Cytotoxicity of flowable resin composite on cultured human periodontal ligament cells compared with mineral trioxide aggregate. J Investig Clin Dent. 2012; 3: 215-20.
  37. Ramezani GH, Moghadam MM, Saghiri MA et al. Effect of dental restorative materials on total antioxidant capacity and calcium concentration of unstimulated saliva. J Clin Exp Dent. 2017; 9: e71-e77.
  38. Durner J, Schrickel K, Watts DC, Ilie N. Determination of homologous distributions of bisEMA dimethacrylates in bulk-fill resin-composites by GC–MS. Dental Materials. 2015; 31: 473-80.
  39. Longo DL, Paula-Silva FW, Faccioli LH, Gaton-Hernandez PM, Queiroz AM, Silva LA. Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins. J Appl Oral Sci. 2016; 24: 338-43.
  40. Ilday NO, Celik N, Dilsiz A et al. The effects of silorane composites on levels of cytokines and periodontal parameters. Contemp Clin Dent. 2013; 4: 437-42.
  41. Fleming GJ, Awan M, Cooper PR, Sloan AJ. The potential of a resin-composite to be cured to a 4mm depth. Dental Materials. 2008; 24: 522-29.
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