Issue

Vol. 63 No. 12: Issue 12

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Evaluation of STAT3 rs1053004 single nucleotide polymorphism in patients with chronic hepatitis B and hepatocellular carcinoma

https://doi.org/10.14715/10.14715/cmb/2017.63.12.11
Maryam Fatemipour
Department of Bacteriology and Virology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Seyed Ali Mohammad Arabzadeh
Department of Bacteriology and Virology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Hamdireza Molaei
Department of Bacteriology and Virology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Bita Geramizadeh
Department of Pathology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Shahryar Dabiri
Department of Bacteriology and Virology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Bahareh Fatemipour
Department of Orthodontics, Faculty of Dentistry, Kerman University of Medical Sciencse, Iran
Seyed Mahdi Vahedi
Department of Periodontics, Faculty of Dentistry, Kerman University of Medical Sciences, Iran
Reza Malekpour Afshar
Department of Bacteriology and Virology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Corresponding Author(s) : Reza Malekpour Afshar

malekpour@kmu.ac.ir

Cellular and Molecular Biology, Vol. 63 No. 12: Issue 12

Abstract

Chronic infection with hepatitis B (CHB) virus is one of the most important risk factors for Hepatocellular carcinoma (HCC). Hepatitis B virus (HBV) causes liver cancer in various ways. One of these ways is increasing the expression of the signal transducer and activator of transcription 3 (STAT3) in Hepatocytes by HBV. The purpose of this research is to evaluate the single nucleotide polymorphism (SNP) rs1053004 in the STAT3 gene in CHB patients and individuals who suffer from HCC. In this research, 33 patients CHB-related HCC, 50 patients infected with chronic hepatitis B infection (CHB) without HCC and 50 healthy individuals were investigated for the presence of rs1053004 in the STAT3 gene according to the PCR-based differentiation of alleles test. Data analysis presented a different and significant distribution of alleles and genotypes (p<0.05). When the HCC and CHB groups were compared from the point of the frequency of alleles, the frequency of the C allele and CC genotype in the HCC group were higher CHB and control groups. Analysis of our data in the genotype model (CC vs. TT + TC) showed, this meaningful relationship remained between the HCC group and the three groups of CHB, healthy and all controls. These results illustrate that perhaps rs1053004 polymorphisms in the STAT3 gene participated in the progression of hepatitis B to HCC in Iranian people.

Keywords

Hepatocellular carcinoma Chronic hepatitis B Single nucleotide polymorphism STAT3.
Fatemipour, M., Arabzadeh, S. A. M., Molaei, H., Geramizadeh, B., Dabiri, S., Fatemipour, B., Vahedi, S. M., & Malekpour Afshar, R. (2017). Evaluation of STAT3 rs1053004 single nucleotide polymorphism in patients with chronic hepatitis B and hepatocellular carcinoma. Cellular and Molecular Biology, 63(12), 45–50. https://doi.org/10.14715/10.14715/cmb/2017.63.12.11
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics, 2002. CA Cancer J Clin. 2005,55(2):74-108.
  2. Kao JH, Chen DS. Global control of hepatitis B virus infection. Lancet Infect Dis. 2002,2(7):395-403.
  3. Levrero M, Zucman-Rossi J. Mechanisms of HBV-induced hepatocellular carcinoma. J Hepatol. 2016,64(1 Suppl):S84-S101. 10.1016/j.jhep.2016.02.021
  4. Moriggl R, Berchtold S, Friedrich K, Standke GJ, Kammer W, Heim M, Wissler M, Stocklin E, Gouilleux F, Groner B. Comparison of the transactivation domains of Stat5 and Stat6 in lymphoid cells and mammary epithelial cells. Mol Cell Biol. 1997,17(7):3663-78.
  5. Garcia R, Bowman TL, Niu G, Yu H, Minton S, Muro-Cacho CA, Cox CE, Falcone R, Fairclough R, Parsons S, Laudano A, Gazit A, Levitzki A, Kraker A, Jove R. Constitutive activation of Stat3 by the Src and JAK tyrosine kinases participates in growth regulation of human breast carcinoma cells. Oncogene. 2001,20(20):2499-513. 10.1038/sj.onc.1204349
  6. Zhu YZ, Zhu R, Fan J, Pan Q, Li H, Chen Q, Zhu HG. Hepatitis B virus X protein induces hypermethylation of p16(INK4A) promoter via DNA methyltransferases in the early stage of HBV-associated hepatocarcinogenesis. J Viral Hepat. 2010,17(2):98-107. 10.1111/j.1365-2893.2009.01156.x
  7. Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI. Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology. 2007,132(4):1476-94. 10.1053/j.gastro.2007.01.034
  8. Herkel J, Jagemann B, Wiegard C, Lazaro JF, Lueth S, Kanzler S, Blessing M, Schmitt E, Lohse AW. MHC class II-expressing hepatocytes function as antigen-presenting cells and activate specific CD4 T lymphocyutes. Hepatology. 2003,37(5):1079-85. 10.1053/jhep.2003.50191
  9. Darnell JE, Jr., Joys and surprises of a career studying eukaryotic gene expression. J Biol Chem. 2013,288(18):12957-66. 10.1074/jbc.X113.471722
  10. Benn J, Su F, Doria M, Schneider RJ. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J Virol. 1996,70(8):4978-85.
  11. Campbell GS, Yu CL, Jove R, Carter-Su C. Constitutive activation of JAK1 in Src-transformed cells. J Biol Chem. 1997,272(5):2591-4.
  12. Waris G, Siddiqui A. Interaction between STAT-3 and HNF-3 leads to the activation of liver-specific hepatitis B virus enhancer 1 function. J Virol. 2002,76(6):2721-9.
  13. Kimchi-Sarfaty C, Oh JM, Kim IW, Sauna ZE, Calcagno AM, Ambudkar SV, Gottesman MM. A "silent" polymorphism in the MDR1 gene changes substrate specificity. Science. 2007,315(5811):525-8. 10.1126/science.1135308
  14. Sauna ZE, Kimchi-Sarfaty C, Ambudkar SV, Gottesman MM. Silent polymorphisms speak: how they affect pharmacogenomics and the treatment of cancer. Cancer Res. 2007,67(20):9609-12. 10.1158/0008-5472.CAN-07-2377
  15. Xie J, Zhang Y, Zhang Q, Han Y, Yin J, Pu R, Shen Q, Lu W, Du Y, Zhao J, Han X, Zhang H, Cao G. Interaction of signal transducer and activator of transcription 3 polymorphisms with hepatitis B virus mutations in hepatocellular carcinoma. Hepatology. 2013,57(6):2369-77. 10.1002/hep.26303
  16. Foshay KM, Gallicano GI. miR-17 family miRNAs are expressed during early mammalian development and regulate stem cell differentiation. Dev Biol. 2009,326(2):431-43. 10.1016/j.ydbio.2008.11.016
  17. Sopipong W, Tangkijvanich P, Payungporn S, Posuwan N, Poovorawan Y. The KIF1B (rs17401966) single nucleotide polymorphism is not associated with the development of HBV-related hepatocellular carcinoma in Thai patients. Asian Pac J Cancer Prev. 2013,14(5):2865-9.
  18. Abe A, Inoue K, Tanaka T, Kato J, Kajiyama N, Kawaguchi R, Tanaka S, Yoshiba M, Kohara M. Quantitation of hepatitis B virus genomic DNA by real-time detection PCR. J Clin Microbiol. 1999,37(9):2899-903.
  19. Hou J, Xu J, Jiang R, Wang Y, Chen C, Deng L, Huang X, Wang X, Sun B. Estrogen-sensitive PTPRO expression represses hepatocellular carcinoma progression by control of STAT3. Hepatology. 2013,57(2):678-88. 10.1002/hep.25980
  20. Afzali F, Heidari Z, Montazeri M, Ahmadian L, Zahedi MJ. Futures studies in health: choosing the best intelligent data mining model to predict and diagnose liver Cancer in early stage. Journal of Health and Biomedical Informatics. 2015,2(3):133-40.
  21. Shamszad M, Farzadegan H. Hepatitis B related cirrhosis and hepatocellular carcinoma in Iran. J Irn Med Council. 1982,8:238.
  22. Fazelipour S, Toutian Z, Davoudi P. The effect of the heroin on histological structure of liver in mouse (Balb/C). Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch. 2006,16(2):91-4.
  23. Choudhari SR, Khan MA, Harris G, Picker D, Jacob GS, Block T, Shailubhai K. Deactivation of Akt and STAT3 signaling promotes apoptosis, inhibits proliferation, and enhances the sensitivity of hepatocellular carcinoma cells to an anticancer agent, Atiprimod. Mol Cancer Ther. 2007,6(1):112-21. 10.1158/1535-7163.MCT-06-0561
  24. Alavian SM, Kabir A, Ahmadi AB, Lankarani KB, Shahbabaie MA, Ahmadzad-Asl M. Hepatitis C infection in hemodialysis patients in Iran: a systematic review. Hemodial Int. 2010,14(3):253-62. 10.1111/j.1542-4758.2010.00437.x
  25. Hajiani E, Masjedizadeh R, Hashemi J, Azmi M, Rajabi T. Risk factors for hepatocellular carcinoma in Southern Iran. Saudi Med J. 2005,26(6):974-7.
  26. Jee SH, Ohrr H, Sull JW, Samet JM. Cigarette smoking, alcohol drinking, hepatitis B, and risk for hepatocellular carcinoma in Korea. J Natl Cancer Inst. 2004,96(24):1851-6. 10.1093/jnci/djh334
  27. Yu H, Pardoll D, Jove R. STATs in cancer inflammation and immunity: a leading role for STAT3. Nat Rev Cancer. 2009,9(11):798-809. 10.1038/nrc2734
  28. Nguyen KB, Watford WT, Salomon R, Hofmann SR, Pien GC, Morinobu A, Gadina M, O'Shea JJ, Biron CA. Critical role for STAT4 activation by type 1 interferons in the interferon-gamma response to viral infection. Science. 2002,297(5589):2063-6. 10.1126/science.1074900
  29. Chanthra N, Payungporn S, Chuaypen N, Pinjaroen N, Poovorawan Y, Tangkijvanich P. Association of Single Nucleotide Polymorphism rs1053004 in Signal Transducer and Activator of Transcription 3 (STAT3) with Susceptibility to Hepatocellular Carcinoma in Thai Patients with Chronic Hepatitis B. Asian Pac J Cancer Prev. 2015,16(12):5069-73.
  30. Messoudi S, Al-Sulaiti MA, Al-Busaidi AS, Dendana M, Nsiri B, Almawi WY, Mahjoub T. Contribution of JAK2 and STAT3 variants to the genetic susceptibility of recurrent miscarriage among Bahraini and Tunisian Arabs. Mol Biol Rep. 2013,40(1):585-9. 10.1007/s11033-012-2096-8
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 948 Download : 388