Issue

Vol. 64 No. 1: Issue 1

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Correlation between serum leptin and its gene expression to the anthropometric measures in overweight and obese children

https://doi.org/10.14715/cmb/2018.64.1.15
Maha K Eldosouky
Department of Anatomy, Faculty of Medicine, Taibah University, KSA
Azza M Abdu Allah
Medical Biochemistry Department, Faculty Of Medicine, Menoufia University, Egypt
Abeer AbdElmoneim
Department of pediatrics, Faculty of Medicine, Taibah University, KSA
Najat Saaeid Al-Ahmadi
Gastroenterology department, Madina obstetric and child hospital, KSA

Corresponding Author(s) : Azza M Abdu Allah

azza.abdallah@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 1: Issue 1

Abstract

Obesity is a multifactor disorder with evidence supporting the role of the genetic factor in its etiology. The present study evaluates the relationship between leptin G2548A (rs7799039) and leptin receptors (Gln223Arg (rs1137101) genotyping and its leptin level and the risk of childhood obesity. This case-control study was conducted on 168 overweight and obese Saudi children and 126 non obese one served as control. Fasting insulin, leptin, blood glucose, lipid profile levels were measured. HOMA- IR, and BMI were assessed. Genotyping of leptin and leptin receptor gene variants was done by SNP real-time PCR method. GG genotype and G allele of rs1137101 were significantly higher in overweight and obese children than controls. It showed significant association with risk of obesity OR 7.1 [ 95% CI: 3.4 – 14.8] and OR 2.8 [ 95% CI: 2.0 – 4.1] respectively. Leptin level was significantly greater in patients than controls (p<0.000*) with GG and AG genotypes having the highest level of leptin when compared with another genotype in the obese group. As regards, rs7799039 AA genotype showed significant higher leptin level than other genotypes in the same group with a non-significant difference in genotypes distribution between obese and controls. rs1137101 variant of leptin receptor and fasting leptin level are correlated with overweight and obesity in Saudi children. The GG genotype of leptin receptors rs1137101 and higher serum leptin levels can be used as risk factors for childhood obesity.

Keywords

Leptin Polymorphism Obese Receptors Real time PCR.
Eldosouky, M. K., Abdu Allah, A. M., AbdElmoneim, A., & Al-Ahmadi, N. S. (2018). Correlation between serum leptin and its gene expression to the anthropometric measures in overweight and obese children. Cellular and Molecular Biology, 64(1), 84–90. https://doi.org/10.14715/cmb/2018.64.1.15
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. -Mirzazadeh A, Sadeghirad B, Haghdoost AA, Bahreini F, Rezazadeh Kermani M. The prevalence of obesity in Iran in recent decade; a systematic review and meta-analysis study. Iran J Public Health. 2009;38(3):1–11.
  2. -Tirosh A, Shai I, Afek A, et al. Adolescent BMI trajectory and risk of diabetes versus coronary disease. N Engl J Med 2011;364:1315-25.
  3. -Dietitians of Canada, Canadian Paediatric Society, The College of Family Physicians of Canada, Community Health Nurses of Canada. Promoting optimal monitoring of child growth in Canada: using the new World Health Organization growth charts. 2010. Available: www.dietitians.ca/growthcharts (accessed 2014 Oct. 24).
  4. -International Obesity Task Force. [accessed on November 22, 2013]. Availablefrom: http://www.iaso.org/iotf/obesity/obesitytheglobalepidemic/ for Saudi, Canada, South Africa, Australia and NZ estimates.
  5. -Ogden CL, Carroll MD, Kit BK, et al. Prevalence of obesity and trends in body mass index among US children and adolescents, 1999-2010. JAMA 2012;307:483-90.
  6. -Shabana and Hasnain S 2016b The p. N103K mutation of Leptin (LEP) gene and severe early onset obesity in Pakistan. Biol. Res. 2016 : 23.
  7. - Cammisotto PG, Bukowiecki LJ, Deshaies Y, Bendayan M. Leptin biosynthetic pathway in white adipocytes. Biochem Cell Biol 2006;84:207–214.
  8. - Hinuy HM, Hirata MH, Forti N et al. Leptin G-2548A promoter polymorphism is associated with increased plasma leptin and BMI in Brazilian women. Arq Bras Endocrinol Metabol 2008;52:611–616.
  9. - Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR, Ohannesian JP, Marco CC, McKee LJ, Bauer TL, et al. Serum Immunoreactive- Leptin Concentrations in Normal Weight and Obese Humans, N Engl J Med. 1996 Feb 1;334(5):292-5
  10. - Judzewitch R, Pfeifer M, Best J, Halter J and Port D. Chronic chlorpropamide therapy of noninsulin-dependent diabetes augments basal and stimulated insulin secretion by increasing islet sensitivity to glucose. J Clin EndocrinolMetab. 55(2):321-8, 1982.
  11. - Matthews D; Hosker J; Rudenski A; Naylor B; Treacher D and Turner R: Homeostasis model assessment: insulin resistance and beta cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 28:412-419, 1985.
  12. -Kumar S and Kelly AS Review of Childhood Obesity: From Epidemiology, Etiology, and Comorbidities to Clinical Assessment and Treatment. Mayo Clin Proc. 2017 Feb;92(2):251-265.
  13. -Bouchard C (2009) Childhood obesity: are genetic differences involved? Am J Clin Nutr 89:1494–1501.
  14. - Farooqi IS, O'Rahilly S (2005) Monogenic obesity in humans. Ann Rev Med 56:443–458.
  15. -Chavarria-Avila E, Vázquez-Del Mercado M, Gomez-Bañuelos E, Ruiz-Quezada SL, Castro-Albarran J, Sánchez-López L, Martí­n-Marquez BT, Navarro-Hernández RE.The Impact of LEP G-2548A and LEPR Gln223Arg Polymorphisms on Adiposity, Leptin, and Leptin-Receptor Serum Levels in a Mexican Mestizo Population. Biomed Res Int. 2015;2015:539408.
  16. -Sabah A. Linjawi and Noor A. Hussain. Impact of Leptin Receptor Gene GLN223ARG Polymorphism on Obesity in Jeddah City. Life Sci J 2012;9(4):818-828.
  17. -Kanjana, Rungsunn Tungtrongchitr and Kittisak Thawnasom Measurement of the levels of leptin, BDNF associated with polymorphisms LEP G2548A, LEPR Gln223Arg and BDNF Val66Met in Thai with metabolic syndrome. Diabetology & Metabolic Syndrome 2014, 6:6.
  18. -Yiannakouris N, Yannakoulia M, Melistas L, Chan JL, Klimis-Zacas D, Mantzoros CS: The Q223R polymorphism of the leptin receptor gene is significantly associated with obesity and predicts a small percentage of body weight and body composition variability. J Clin Endocrinol Metab 2001, 86:4434–4439.
  19. -Richert, L., Chevalley, T., Manen, D., Bonjour,J., Rizzoli, R. and Ferrari, S. (2007): Bone Mass in Prepubertal Boys Is Associated with a Gln223Arg Amino Acid Substitution in the Leptin Receptor, The Journal of Clinical Endocrinology & Metabolism, 92(11), p 4380-4386.
  20. - Utsunomiya K, Shinkai T, Sakata S, Hwang R, Yamada K, Chen HI, Fukunaka Y, Ohmori O, Nakamura J: Lack of association between the leptin receptor gene (LEPR) Gln223Arg polymorphism and late-onset Alzheimer disease.Alzheimer Dis Assoc Disor 2010, 24:101–103.
  21. -Guí­zar-Mendoza JM1, Amador-Licona N, Flores-Martí­nez SE, López-Cardona MG, Ahuatzin-Trémary R, Sánchez-Corona J. Association analysis of the Gln223Arg polymorphism in the human leptin receptor gene, and traits related to obesity in Mexican adolescents. J Hum Hypertens. 2005 May;19(5):341-6.
  22. -Okada T1, Ohzeki T, Nakagawa Y, Sugihara S, Arisaka O; Study Group of Pediatric Obesity and Its related Metabolism. Impact of leptin and leptin-receptor gene polymorphisms on serum lipids in Japanese obese children. Acta Paediatr. 2010 Aug;99(8):1213-7.
  23. -Komşu-Ornek Z1, Demirel F, Dursun A, Ermiş B, Pişkin E, Bideci A. Leptin receptor gene Gln223Arg polymorphism is not associated with obesity and metabolic syndrome in Turkish children. Turk J Pediatr. 2012 Jan-Feb;54(1):20-4.
  24. -Boumaiza I, Omezzine A, Rejeb J, Rebhi L, Ouedrani A, Ben Rejeb N, Nabli N, Ben Abdelaziz A, et al. 2012 Relationship between leptin G2548A and leptin receptor Q223R gene polymorphisms and obesity and metabolic syndrome risk in Tunisian volunteers. Genet. Test Mol. Biomarkers 16 726–733.
  25. -Amina Alnory, Hoda Gad,, Gehan Hegazy, Olfat Shaker The association of vaspin rs2236242 and leptin rs7799039 polymorphism with metabolic syndrome in Egyptian women. Turk J Med Sci (2016) 46: 1335-1340.
  26. -Shahid A, Rana S, Mahmood S and Saeed S 2015 Role of leptin G-2548A polymorphism in age- and gender-specific development of obesity.
  27. J. Biosci. September 2015,40; 521–530.
  28. -Hoffstedt J, Eriksson P, Mottagui-Tabar S, Arner P: A polymorphism in the leptin promoter region (-2548 G/A) influences gene expression and adipose tissue secretion of leptin. Horm Metab Res 2002, 34:355–359.
  29. -Ng ZY, Veerapen MK, HonWMand Lim RL 2014 Association of leptin/receptor and TNF-α gene variants with adolescent obesity in Malaysia. Pediatr. Int. doi:10.1111/ped.12336.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 1099 Download : 463

Most read articles by the same author(s)