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Vol. 64 No. 6: Advances in mechanism and treatment strategy of cancer

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Flavored Guilu Erxian decoction inhibits the injury of human bone marrow mesenchymal stem cells induced by cisplatin

https://doi.org/10.14715/cmb/2018.64.6.11
Bin Ke
Department of Traditional Chinese Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
Lin Shi
Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, China
Zhaozhong Xu
Department of Emergency, Zhujiang Hospital of Southern Medical University, Guangzhou, China
Gang Wu
Department of Cancer Center, Zhujiang Hospital of Southern Medical University, Guangzhou, China
Yuxin Gong
Department of Respiratory Diseases, Zhujiang Hospital of Southern Medical University, Guangzhou, China
Lixia Zhu
Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, China
Yanjing Wang
Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, China
Huailiang Ouyang
Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, China
Xilong Wang
Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, China

Corresponding Author(s) : Lin Shi

Shilin293@163.com

Cellular and Molecular Biology, Vol. 64 No. 6: Advances in mechanism and treatment strategy of cancer

Abstract

To examine the exact role of flavored Guilu Erxian decoction, a Traditional Chinese Medicine (TCM) in the treatment of cisplatin-induced side-effects in bone marrow mesenchymal stem cells (BM-MSCs). BM-MSCs were isolated from bone marrow collected from SD rats and identified by flow cytometry. Cells were cultivated in MEM alpha medium containing 5% (TCM-L), 10% (TCM-M) and 20% (TCM-H) dosages of flavored Guilu Erxian decoction with or without cisplatin. Cell viability was determined through CCK-8 and thymidine analog 5-ethynyl-2"²-deoxyuridine (EdU) staining assay. Flow cytometry was used to determine cell cycle and apoptosis. The expression of p21 and cleaved-caspase-3 were examined using Western blot assay. The PI3K-AKT-mTOR pathway associated proteins, including p-PI3K, p-AKT and p-mTOR, were also examined by Western blot assay. CCK-8 and EdU staining assay demonstrated that cisplatin could inhibit cell proliferation in BM-MSCs in a dose and time dependent manner. Further, cisplatin could induce apoptosis through increasing G0/G1 cell cycle arrest, p21 and cleaved-caspase-3 expression. However, these phenomena would be significantly alleviated when adding the serum containing flavored Guilu Erxian decoction. Furthermore, the PI3K-AKT-mTOR pathway activation could be inhibited by cisplatin in BM-MSCs, while flavored Guilu Erxian decoction treatment successfully abrogated this effect. Combination of flavored Guilu Erxian decoction and cisplatin could reduce the damage to BM-MSCs. This indicates that the flavored Guilu Erxian decoction can enhance the possibility of BM-MSCs repairing and rehabilitating the normal function of injured tissues induced by cisplatin, which could provide a new direction for therapeutic applications.

Keywords

Flavored Guilu Erxian decoction Traditional Chinese Medicine (TCM) Cisplatin BM-MSCs Proliferation Apoptosis.
Ke, B., Shi, L., Xu, Z., Wu, G., Gong, Y., Zhu, L., Wang, Y., Ouyang, H., & Wang, X. (2018). Flavored Guilu Erxian decoction inhibits the injury of human bone marrow mesenchymal stem cells induced by cisplatin. Cellular and Molecular Biology, 64(6), 58–64. https://doi.org/10.14715/cmb/2018.64.6.11
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