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Vol. 64 No. 10: Issue 10

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Warning: spread of NDM-1 in two border towns of Iran

https://doi.org/10.14715/cmb/2018.64.10.20
Shahnaz Armin
Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Fatemeh Fallah
Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Leila Azimi
Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Hossein Samadi Kafil
Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran 
Kiarash Ghazvini
Department of Microbiology and Virology, Antimicrobial Resistance Research Center, Avicenna Research Institute, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Sepide Hasanzadeh
Department of Microbiology and Virology. Mashhad University of Medical Sciences, Mashhad, Iran
Abdollah Karimi
Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Corresponding Author(s) : Leila Azimi

leilaazimi1982@gmail.com

Cellular and Molecular Biology, Vol. 64 No. 10: Issue 10

Abstract

NDM-1 producing gram-negative bacteria can be resistant to every beta-lactam antibiotic, including carbapenem which is one of the last-lines of antibiotic therapy against multi-drug resistant bacteria. This study aimed to detect the metallo-beta-lactamase in the isolated gram-negative bacteria of the Iranian clinical specimens collected from two major cities in Iran. In this cross sectional study 171 Acinetobacter baumannii, 120 Enterobacter spp. and 145 Klebsiella pneumoniae isolated from clinical specimens of two training hospitals in Tabriz and Mashhad were evaluated. Carbapenem resistant screening was performed according to CLSI guide line. The antibiotic susceptibility testing was prepared for carbapenem resistant strains. Then, the metallo-beta- lactamase genes detection was also carried out by PCR assay and confirmed by sequencing. Sixty-eight, 12 and 22 carbapenem resistant Acinetobacter baumannii, Klebsiella pneumoniae and Enterobacter spp. were respectively confirmed, respectively. blaVIM in 9% and blaNDM-1  in 4% of isolated A. baumannii were observed. blaNDM-1 was also detected in 18% and 25% of K. pneumoniae and Enterobacter spp. isolates, respectively. This is the first report of NDM-1 producer A. baumannii and Enterobacter pp. in Iran. NDM-1 producing gram-negative bacteria can be resistant to all beta-lactam antibiotics and cause complicated challenges in health care systems.

Keywords

NDM-1 Acinetobacter baumannii Enterobacter spp. Iran.
Armin, S., Fallah, F., Azimi, L., Samadi Kafil, H., Ghazvini, K., Hasanzadeh, S., & Karimi, A. (2018). Warning: spread of NDM-1 in two border towns of Iran. Cellular and Molecular Biology, 64(10), 125–129. https://doi.org/10.14715/cmb/2018.64.10.20
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