Effect of HOTAIR rs12826786 and rs1899663 polymorphisms on lung cancer susceptibility and clinicopathological characteristics in a turkish population: a hospital-based case-control study

Erdoğan Dadaş; Muhsin Aydın

doi:10.14715/cmb/2018.64.7.17

Issue

Vol. 64 No. 7: Issue 7

Issue Published : June 12, 2018

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Effect of HOTAIR rs12826786 and rs1899663 polymorphisms on lung cancer susceptibility and clinicopathological characteristics in a turkish population: a hospital-based case-control study

https://doi.org/10.14715/cmb/2018.64.7.17

Erdoğan Dadaş

Department of Thoracic Surgery, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey

Muhsin Aydın

Department of Biology, Faculty of Science and Letters, Adiyaman University, Adiyaman, Turkey

http://orcid.org/0000-0002-1204-1163

Corresponding Author(s) : Erdoğan Dadaş

erdogandadas@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 7: Issue 7
Article Published : May 30, 2018

Abstract

Overexpression of Hox transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with tumorigenesis and multiple cancer types including lung cancer. In this study, the association between two HOTAIR single nucleotide polymorphisms (SNPs) (rs12826786 and rs1899663) on the risk and clinical characteristics of lung cancer in a Turkish population was investigated. We genotyped HOTAIR rs12826786 and rs1899663 polymorphisms in 180 Turkish people including 87 lung cancer patients (71 males and 16 females) and 93 age-matched healthy controls (67 males and 26 females) by a TaqMan real-time polymerase chain reaction method. The mean age value of the lung cancer patients and control subjects were 59.27 ± 10.55 and 61.77 ± 12.00, respectively. We found that none of the two HOTAIR polymorphisms (rs12826786 T>C, rs1899663A>C) has any significant association with the increased risk of lung cancer in any type of inheritance genetic models. However, our research indicated that carriers of Trs12826786/Crs1899663 (ht3) (P = 0.03) had an increased risk of lung cancer susceptibility.

Keywords

Genetic susceptibility HOTAIR Lung cancer HOTAIR rs12826786 polymorphism HOTAIR rs1899663 polymorphism.

Dadaş, E., & Aydın, M. (2018). Effect of HOTAIR rs12826786 and rs1899663 polymorphisms on lung cancer susceptibility and clinicopathological characteristics in a turkish population: a hospital-based case-control study. Cellular and Molecular Biology, 64(7), 97–102. https://doi.org/10.14715/cmb/2018.64.7.17

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References

Liu W, Yin NC, Liu H, Nan KJ. Cav-1 promote lung cancer cell proliferation and invasion through lncRNA HOTAIR. Gene. 2018; 641: 335–40.
Siegel RL, Naishadham D, Jemal A. Cancer statistics, 201. CA Cancer J Clin. 2012; 62: 10-29.
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA Cancer J Clin. 2016; 66: 7–30.
Popper HH. Progression and metastasis of lung cancer. Cancer Metastasis Rev. 2016; 35: 75–91.
Verdecchia A, Francisci S, Brenner H, Gatta G, Micheli A, Mangone L, Kunkler I, and EUROCARE-4 Working Group. Recent cancer survival in Europe: a 2000-02 period analysis of EUROCARE- 4 data. Lancet Oncol. 2007; 8: 784–96.
Reck M, Heigener DF, Mok T, Soria JC, Rabe KF. Management of non-small-cell lung cancer: recent developments. Lancet. 2013; 382, 709–19.
Chen J, Wang R, Zhang K, Chen L-B. Long non-coding RNAs in non-small cell lung cancer as biomarkers and therapeutic targets. J Cell Mol Med. 2014; 18(12): 2425-36.
Brosnan CA, voinnet O. The long and the short of noncoding RNAs. Curr Opin Cell Biol. 2009; 21: 416-25.
Moazed D. Small RNAs in transcriptional gene silencing and genome defence. Nature 2009; 457: 413-20.
Malone CD, Hannon GJ. Small RNAs as guardians of the genome. Cell. 2009; 136: 656-68.
Zhao W, An Y, Liang Y, Xie XW. Role of HOTAIR long noncoding RNA in metastatic progression of lung cancer. Eur Rev Med Pharmacol Sci. 2014; 18(13): 1930-6.
Gong WJ, Yin JY, Li XP, Fang C, Xiao D, Zhang W, Zhou HH, Li Xi, Liu ZQ. Association of well-characterized lung cancer lncRNA polymorphisms with lung cancer susceptibility and platinum-based chemotherapy response. Tumour Biol. 2016; 37(6): 8349-58.
Tao T, Li G, Zhang X, Guan H, Huang Y, Chen M. Association between LncRNA HOTAIR rs4759314 A > G polymorphism and cancer risk: a meta-analysis based on 5525 cases and 6657 controls in Chinese populations. Int J Clin Exp Med 2016; 9(7): 12780-7.
GeneCards. https://www.genecards.org/cgi-bin/carddisp.pl?gene=HOTAIR (Accessed on March 10, 2018).
Edge SB, Compton CC. The American Joint Committee on Cancer: the 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 2010; 17(6): 1471–4.
Gauderman WJ, Morrison JM. Quanto 1.1: A computer program for power and sample size calculations for genetic-epidemiology studies. 2006. http://hydra.usc.edu/gxe (Accessed on January 20, 2018).
Rodriguez S, Gaunt TR, Day IN. Hardy–Weinberg equilibrium testing of biological ascertainment for Mendelian randomization studies. Am J Epidemiol. 2009; 169(4): 505–14.
Sole´ X, Guino´ E, Valls J, Iniesta R, Moreno V. SNPStats: a web tool for the analysis of association studies. Bioinformatics. 2006; 22(15): 1928–9.
Wapinski O, Chang HY. Long noncoding RNAs and human disease. Trends Cell Biol. 2011; 21: 354-61.
Kim K, Jutooru I, Chadalapaka G, Johnson G, Frank J, Burghardt R, Kim S, Safe S. HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene. 2013; 32: 1616-25.
Geng YJ, Xie SL, Li Q, Ma J, Wang GY. Large intervening non-coding RNA HOTAIR is associated with hepatocellular carcinoma progression. J Int Med Res. 2011; 39: 2119-28.
Niinuma T, Suzuki H, Nojima M, Nosho K, Yamamoto H, Takamaru H, Yamamoto E, Association between LncRNA HOTAIR rs4759314 A > G polymorphism and cancer risk 12787 Int J Clin Exp Med. 2016; 9(7): 12780-7.
Maruyama R, Nobuoka T, Miyazaki Y, Nishida T, Bamba T,Kanda T, Ajioka Y, Taguchi T, Okahara S, Takahashi H, Nishida Y, Hosokawa M, Hasegawa T, Tokino T, Hirata K, Imai K, Toyota M, Shinomura Y. Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors. Cancer Res. 2012; 72: 1126-36.
Hajjari M, Behmanesh M, Sadeghizadeh M, Zeinoddini M. Up-regulation of HOTAIR long non-coding RNA in human gastric adenocarcinoma tissues. Med Oncol. 2013; 30: 670.
Bayram S, Ulger Y, Sumbul AT, Kaya BY, Rencüzoğulları A, Genç A, Sevgiler Y, Bozkurt O, Rencüzoğulları E. A functional HOTAIR rs920778 polymorphism does not contributes to gastric cancer in a Turkish population: a case-control study. Fam Cancer. 2015; 14:561-7.
Li X, Wu Z, Mei Q, Li X, Guo M, Fu X, Han W. Long non-coding RNA HOTAIR, a driver of malignancy, predicts negative prognosis and exhibits oncogenic activity in oesophageal squamous cell carcinoma. Br J Cancer. 2013; 109: 2266-78.
Chen FJ, Sun M, Li SQ, Wu QQ, Ji L, Liu ZL, Zhou GZ, Cao G, Jin L, Xie HW, Wang CM, Lv J, De W, Wu M, Cao XF. Upregulation of the long non-coding RNA HOTAIR promotes esophageal squamous cell carcinoma metastasis and poor prognosis. Mol Carcinog. 2013; 52: 908-15.
Chisholm KM, Wan Y, Li R, Montgomery KD, Chang HY and West RB. Detection of long noncoding RNA in archival tissue: correlation with polycomb protein expression in primary and metastatic breast carcinoma. PLoS One 2012; 7: e47998.
Bayram S, Sumbul AT, Batmaci CY and Genc A. Effect of HOTAIR rs920778 polymorphism on breast cancer susceptibility and clinicopathologic features in a Turkish population. Tumour Biol. 2015; 36: 3863-70.
Kogo R, Shimamura T, Mimori K, Kawahara K, Imoto S, Sudo T, Tanaka F, Shibata K, Suzuki A, Komune S, Miyano S, Mori M. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer Res. 2011; 71: 6320-26.
Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N, Sato I, Takahashi S, Kondo T, Satoh K. Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer. Biochem Biophys Res Commun. 2013; 436: 319-24.
Liu XH, Liu ZL, Sun M, Liu J, Wang ZX and De W. The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer. BMC Cancer. 2013; 13: 464.
Li D, Feng J, Wu T, Wang Y, Sun Y, Ren J, Liu M. Long intergenic noncoding RNA HOTAIR is overexpressed and regulates PTEN methylation in laryngeal squamous cell carcinoma. Am J Pathol 2013; 182: 64-70.
Nie Y, Liu X, Qu S, Song E, Zou H and Gong C. Long non-coding RNA HOTAIR is an independent prognostic marker for nasopharyngeal carcinoma progression and survival. Cancer Sci 2013; 104: 458-64.
Min L, Mu X, Tong A, Qian Y, Ling C, Yi T, Zhao X. The association between HOTAIR polymorphisms and cancer susceptibility: an updated systemic review and meta-analysis. Oncol Targets Ther. 2018; 11: 791-800.
íœlger Y, Dadaş E, Yalinbaş Kaya B, Sümbül AT, Genç A, Bayram S. The analysis of lncRNA HOTAIR rs12826786 C>T polymorphism and gastric cancer susceptibility in a Turkish population: lack of any association in a hospital-based case–control study. Ir J Med Sci. 2017; 186: 859-65.
Bayram S, Sumbul AT, Dadas E. A functional HOTAIR rs12826786 C.T polymorphism is associated with breast cancer susceptibility and poor clinicopathological characteristics in a Turkish population: a hospital-based case-control study. Tumor Biol. 2016; 37(4): 5577–84.
Guo W, Dong Z, Bai Y, Guo Y, Shen S, Kuang G, Xu J. Associations between polymorphisms of HOTAIR and risk of gastric cardia adenocarcinoma in a population of north China. Tumor Biol 2015: 36: 2845-54.
Li J, Cui Z, Li H, Lv X, Gao M, Yang Z, Bi Y, Zhou B, Yin Z. Long non-coding RNA HOTAIR polymorphism and susceptibility to cancer: an updated meta-analysis. Environ Health Prev Med. 2018; 23:8.

References

Liu W, Yin NC, Liu H, Nan KJ. Cav-1 promote lung cancer cell proliferation and invasion through lncRNA HOTAIR. Gene. 2018; 641: 335–40.

Siegel RL, Naishadham D, Jemal A. Cancer statistics, 201. CA Cancer J Clin. 2012; 62: 10-29.

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA Cancer J Clin. 2016; 66: 7–30.

Popper HH. Progression and metastasis of lung cancer. Cancer Metastasis Rev. 2016; 35: 75–91.

Verdecchia A, Francisci S, Brenner H, Gatta G, Micheli A, Mangone L, Kunkler I, and EUROCARE-4 Working Group. Recent cancer survival in Europe: a 2000-02 period analysis of EUROCARE- 4 data. Lancet Oncol. 2007; 8: 784–96.

Reck M, Heigener DF, Mok T, Soria JC, Rabe KF. Management of non-small-cell lung cancer: recent developments. Lancet. 2013; 382, 709–19.

Chen J, Wang R, Zhang K, Chen L-B. Long non-coding RNAs in non-small cell lung cancer as biomarkers and therapeutic targets. J Cell Mol Med. 2014; 18(12): 2425-36.

Brosnan CA, voinnet O. The long and the short of noncoding RNAs. Curr Opin Cell Biol. 2009; 21: 416-25.

Moazed D. Small RNAs in transcriptional gene silencing and genome defence. Nature 2009; 457: 413-20.

Malone CD, Hannon GJ. Small RNAs as guardians of the genome. Cell. 2009; 136: 656-68.

Zhao W, An Y, Liang Y, Xie XW. Role of HOTAIR long noncoding RNA in metastatic progression of lung cancer. Eur Rev Med Pharmacol Sci. 2014; 18(13): 1930-6.

Gong WJ, Yin JY, Li XP, Fang C, Xiao D, Zhang W, Zhou HH, Li Xi, Liu ZQ. Association of well-characterized lung cancer lncRNA polymorphisms with lung cancer susceptibility and platinum-based chemotherapy response. Tumour Biol. 2016; 37(6): 8349-58.

Tao T, Li G, Zhang X, Guan H, Huang Y, Chen M. Association between LncRNA HOTAIR rs4759314 A > G polymorphism and cancer risk: a meta-analysis based on 5525 cases and 6657 controls in Chinese populations. Int J Clin Exp Med 2016; 9(7): 12780-7.

GeneCards. https://www.genecards.org/cgi-bin/carddisp.pl?gene=HOTAIR (Accessed on March 10, 2018).

Edge SB, Compton CC. The American Joint Committee on Cancer: the 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 2010; 17(6): 1471–4.

Gauderman WJ, Morrison JM. Quanto 1.1: A computer program for power and sample size calculations for genetic-epidemiology studies. 2006. http://hydra.usc.edu/gxe (Accessed on January 20, 2018).

Rodriguez S, Gaunt TR, Day IN. Hardy–Weinberg equilibrium testing of biological ascertainment for Mendelian randomization studies. Am J Epidemiol. 2009; 169(4): 505–14.

Sole´ X, Guino´ E, Valls J, Iniesta R, Moreno V. SNPStats: a web tool for the analysis of association studies. Bioinformatics. 2006; 22(15): 1928–9.

Wapinski O, Chang HY. Long noncoding RNAs and human disease. Trends Cell Biol. 2011; 21: 354-61.

Kim K, Jutooru I, Chadalapaka G, Johnson G, Frank J, Burghardt R, Kim S, Safe S. HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene. 2013; 32: 1616-25.

Geng YJ, Xie SL, Li Q, Ma J, Wang GY. Large intervening non-coding RNA HOTAIR is associated with hepatocellular carcinoma progression. J Int Med Res. 2011; 39: 2119-28.

Niinuma T, Suzuki H, Nojima M, Nosho K, Yamamoto H, Takamaru H, Yamamoto E, Association between LncRNA HOTAIR rs4759314 A > G polymorphism and cancer risk 12787 Int J Clin Exp Med. 2016; 9(7): 12780-7.

Maruyama R, Nobuoka T, Miyazaki Y, Nishida T, Bamba T,Kanda T, Ajioka Y, Taguchi T, Okahara S, Takahashi H, Nishida Y, Hosokawa M, Hasegawa T, Tokino T, Hirata K, Imai K, Toyota M, Shinomura Y. Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors. Cancer Res. 2012; 72: 1126-36.

Hajjari M, Behmanesh M, Sadeghizadeh M, Zeinoddini M. Up-regulation of HOTAIR long non-coding RNA in human gastric adenocarcinoma tissues. Med Oncol. 2013; 30: 670.

Bayram S, Ulger Y, Sumbul AT, Kaya BY, Rencüzoğulları A, Genç A, Sevgiler Y, Bozkurt O, Rencüzoğulları E. A functional HOTAIR rs920778 polymorphism does not contributes to gastric cancer in a Turkish population: a case-control study. Fam Cancer. 2015; 14:561-7.

Li X, Wu Z, Mei Q, Li X, Guo M, Fu X, Han W. Long non-coding RNA HOTAIR, a driver of malignancy, predicts negative prognosis and exhibits oncogenic activity in oesophageal squamous cell carcinoma. Br J Cancer. 2013; 109: 2266-78.

Chen FJ, Sun M, Li SQ, Wu QQ, Ji L, Liu ZL, Zhou GZ, Cao G, Jin L, Xie HW, Wang CM, Lv J, De W, Wu M, Cao XF. Upregulation of the long non-coding RNA HOTAIR promotes esophageal squamous cell carcinoma metastasis and poor prognosis. Mol Carcinog. 2013; 52: 908-15.

Chisholm KM, Wan Y, Li R, Montgomery KD, Chang HY and West RB. Detection of long noncoding RNA in archival tissue: correlation with polycomb protein expression in primary and metastatic breast carcinoma. PLoS One 2012; 7: e47998.

Bayram S, Sumbul AT, Batmaci CY and Genc A. Effect of HOTAIR rs920778 polymorphism on breast cancer susceptibility and clinicopathologic features in a Turkish population. Tumour Biol. 2015; 36: 3863-70.

Kogo R, Shimamura T, Mimori K, Kawahara K, Imoto S, Sudo T, Tanaka F, Shibata K, Suzuki A, Komune S, Miyano S, Mori M. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer Res. 2011; 71: 6320-26.

Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N, Sato I, Takahashi S, Kondo T, Satoh K. Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer. Biochem Biophys Res Commun. 2013; 436: 319-24.

Liu XH, Liu ZL, Sun M, Liu J, Wang ZX and De W. The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer. BMC Cancer. 2013; 13: 464.

Li D, Feng J, Wu T, Wang Y, Sun Y, Ren J, Liu M. Long intergenic noncoding RNA HOTAIR is overexpressed and regulates PTEN methylation in laryngeal squamous cell carcinoma. Am J Pathol 2013; 182: 64-70.

Nie Y, Liu X, Qu S, Song E, Zou H and Gong C. Long non-coding RNA HOTAIR is an independent prognostic marker for nasopharyngeal carcinoma progression and survival. Cancer Sci 2013; 104: 458-64.

Min L, Mu X, Tong A, Qian Y, Ling C, Yi T, Zhao X. The association between HOTAIR polymorphisms and cancer susceptibility: an updated systemic review and meta-analysis. Oncol Targets Ther. 2018; 11: 791-800.

íœlger Y, Dadaş E, Yalinbaş Kaya B, Sümbül AT, Genç A, Bayram S. The analysis of lncRNA HOTAIR rs12826786 C>T polymorphism and gastric cancer susceptibility in a Turkish population: lack of any association in a hospital-based case–control study. Ir J Med Sci. 2017; 186: 859-65.

Bayram S, Sumbul AT, Dadas E. A functional HOTAIR rs12826786 C.T polymorphism is associated with breast cancer susceptibility and poor clinicopathological characteristics in a Turkish population: a hospital-based case-control study. Tumor Biol. 2016; 37(4): 5577–84.

Guo W, Dong Z, Bai Y, Guo Y, Shen S, Kuang G, Xu J. Associations between polymorphisms of HOTAIR and risk of gastric cardia adenocarcinoma in a population of north China. Tumor Biol 2015: 36: 2845-54.

Li J, Cui Z, Li H, Lv X, Gao M, Yang Z, Bi Y, Zhou B, Yin Z. Long non-coding RNA HOTAIR polymorphism and susceptibility to cancer: an updated meta-analysis. Environ Health Prev Med. 2018; 23:8.

Author biographies is not available.