Issue

Vol. 64 No. 15: Issue 15

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Neuronal survival of DRG neurons after neurite transection in vitro promotes by nerve growth factor and brain derived neurotrophic factor

https://doi.org/10.14715/cmb/2017.64.15.7
Elif Kaval Oğuz
Department of Science Education, Van Yüzüncü Yıl University, 65080, Van, Turkey

Corresponding Author(s) : Elif Kaval Oğuz

ekoguz@yyu.edu.tr

Cellular and Molecular Biology, Vol. 64 No. 15: Issue 15

Abstract

Neurotrophic factors are growth factors that promote neuronal survival, regulate synaptic function and neurotransmitter release, and promote the plasticity and growth of axons in the peripheral and central nervous systems. This study focused on the roles of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in the survival of adult dorsal root ganglion (DRG) neurons following axotomy. To investigate this, we cultured adult mouse DRG neurons and administered NGF or BDNF to the culture medium at different doses before transection. After determining the optimal doses of NGF and BDNF, these factors were then applied in combination. Axotomy was performed using a precise laser beam and neuronal death was visualized through cell observer microscopy system, by adding propidium iodide to the culture medium. The results demonstrate that the optimal doses of NGF and BDNF for neuronal survival are 150 ng/mL and 50 ng/mL, respectively. The highest level of neuronal survival was observed in the cells treated with a combination of NGF and BDNF. In conclusion, NGF and BDNF have a positive effect, both individually and in combination, on the survival of DRG neurons following neurite transection.

Keywords

Dorsal root ganglion NGF BDNF Neuronal survival Axonal injury.
Kaval Oğuz, E. (2018). Neuronal survival of DRG neurons after neurite transection in vitro promotes by nerve growth factor and brain derived neurotrophic factor. Cellular and Molecular Biology, 64(15), 41–46. https://doi.org/10.14715/cmb/2017.64.15.7
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Aloe L, Rocco ML, Bianchi P, Mani L. Nerve growth factor: from the early discoveries to the potential clinical use. Journal of Translational Medicine 2012; 10; 239.
  2. Barde YA, Edgar D, Thoenen H. Purification of a new neurotrophic factor from mammalian brain. EMBO J 1982; 1; 549-53.
  3. Bibel M. and Barde YA. Neurotrophins: key regulators of cell fate and shape in the vertebrate nervous system. Genes &Development 2000; 14; 2919-37.
  4. Blizzard CA, Haas MA, Vickers JC, Dickson TC. Cellular Dynamics underlying regeneration of damaged axons differs from initial axon development. Eur J Neurosci 2007; 26; 1100–108.
  5. Campenot RB. Development of sympathetic neurons in compartmentalized cultures. I. Local control of neurite growth by nerve growth factor. Dev Biol 1982; 93; 1–12.
  6. Caporali A, Sala-Newby GB, Meloni M, Graiani G, Pani E, Cristofaro B, Newby AC, Madeddu P, Emanueli C. Identification of the prosurvival activity of nerve growth factor on cardiac myocytes. Cell Death Differ 2008; 15; 299–311.
  7. Cengiz N, í–ztürk G, Erdoğan E, Him A, Kaval Oğuz E. Consequences of Neurite Transection In Vitro. Journal of Neurotrauma 2012; 29; 2465-74.
  8. Chen SQ, Cai Q, Shen YY, Cai XY, Lei HY. Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro. Int J Devl Neuroscience 2014; 38; 74-78.
  9. Chu GK. and Tator CH. Calcium influx is necessary for optimal regrowth of transected neurites of rat sympathetic ganglion neurons in vitro. Neuroscience 2001; 102; 945-57.
  10. Chuckowree JA. and Vickers JC. Cytoskeletal and morphological alterations underlying axonal sprouting after localized transection of cortical neuron axons in vitro. J Neurosci 2003; 23; 3715–25.
  11. Cohen S. Purification of a nerve-growth promoting protein from the mouse salivary gland and its neurocytotoxic antiserum. Proc. Natl. Acad. Sci. 1960; 46, 302-11.
  12. Crowley C, Spencer SD, Nishimura MC, Chen, KS, Pitts-Meek S, Armanini MP, Ling LH, McMahon SB, Shelton DL, Levinson AD, Philips H. Mice lacking nerve growth factor display perinatal loss of sensory and sympathetic neurons yet develop basal forebrain cholinergic neurons. Cell 1994; 76; 10001-11.
  13. de Munter JP, Melamed E, Wolters E. Stem cell grafting in parkinsonism–why, how and when. Parkinsonism Relat Disord 2014; 20 (Suppl 1); 150–53.
  14. Dickson TC, Adlard PA, Vickers JC. Sequence of cellular changes following localized axotomy to cortical neurons in glia-free culture. J Neurotrauma 2000; 17; 1095–103.
  15. Durand C, Robin C, Bollerot K, Baron MH, Ottersbach K, Dzierzak E. Embryonic stromal clones reveal developmental regulators of definitive hematopoietic stem cells. Proc Natl Acad Sci 2007; 104; 20838–43
  16. Eddleman CS, Ballinger ML, Smyers ME, Godell CM, Fishman HM, Bittner GD. Repair of plasmalemmal lesions by vesicles. Proc Natl Acad Sci 1997; 94; 4745–50.
  17. Ernfors P, Lee KF, Jaenisch R. Mice lacking brain-derived neurotrophic factor develop with sensory deficits. Nature 1994; 368; 147-50.
  18. Fry EJ, Ho C, David S. A role for Nogo receptor in macrophage clearance from injured peripheral nerve. Neuron 2007; 53; 649-62.
  19. Fu YS. and Gordon T. The cellular and molecular basis of periferal nerve regeneration. Mol Neurobiology 1997; 14 (1); 67-116.
  20. Gallo G. Myosin II activity is required for severing-induced axon retraction in vitro. Exp Neurol 2004; 189; 112–21.
  21. Geddes DM, LaPlaca MC, Cargill RS. Susceptibility of hippocampal neurons to mechanically induced injury. Exp Neurol 2003; 184; 420–27.
  22. Gross GW, Lucas JH, Higgins ML. Laser microbeam surgery: ultrastructural changes associated with neurite transection in culture. J Neurosci 1983; 3; 1979-93.
  23. Hall J, Thomas KL, Everitt BJ. Rapid and selective induction of BDNF expression in hippocampus during contextual learning. Nature Neuroscience 2000; 3; 533-35.
  24. Hashimoto K, Shimizu E, Iyo M. Critical role of brain-derived neurotrophic factor in mood disorders. Brain Research reviews 2004; 45; 104-14.
  25. Heese K, Low JW, Inoue N. Nerve growth factor, neural stem cells and Alzheimer's disease. Neuro-Signals 2006; 15; 1–12
  26. Hendry IA. The response of adrenergic neurons to axotomy and nerve growth factor. Brain Res 1975; 94; 87-97.
  27. Hendry IA. and Campbell J. Morphometric analysis of rat superior cervical ganglion ofter axotomy and nerve growth factor treatment. J Neurocytol 1976; 5; 531-60.
  28. Higgins ML, Smith MN, Gross GW. Selective cell destruction and precise neurite transection in neuroblastoma cultures with pulsed ultraviolet laser microbeam irradiation: an analysis of mechanisms and scanning electron microscopy. J Nerosci Methods 1980; 3; 83-99.
  29. Hu Y, Cui Q, Harvey AR. Interactive effects of C3, cyclic AMP and ciliaryneurotrophic factor on adult retinal ganglion cell survival and axonal regeneration. Mol Cell Neurosci 2007; 34; 88-98.
  30. Huang EJ. and Reichardt LF. Neurotrophins: Roles in development and function. Annu Rev Neurosci 2001; 24; 677-736.
  31. Keefe KM, Sheikh IM. Smith GM. Targetting neurotrophins to specific populations of neurons: NGF, BDNF and NT-3 and their relevance for treatment of spinal cord injury. Int J Mol Sci 2017; 18; 548, doi: 3390/ ijms 18030548.
  32. Kirkpatrick JB, Higgins ML, Lucas JH, Gross GW. In vitro simulation of neural trauma by laser. Exp Neurol 1985; 44; 268-84.
  33. Kirschenbaum B. and Goldman SA. Brain-derived neurotrophic factor promotes the survival of neurons arising from the adult rat forebrain subependymal zone. Neurobiology 1995; 92; 210-14.
  34. Klein R, Smeyne RJ, Wurst W, Long LK, Auerbach BA, Joyner AL, Barbacid M. Targeted disruption of the trkB neurotrophin receptor gene results in nervous system lesions and neonatal death. Cell 1993; 75; 113-22.
  35. Levi-Montalcini R. and Angeletti PV. Nerve growth factor. Physiological Review 1968; 48; 534-69.
  36. Levi-Montalcini R. and Hamburger V. Selective growth-stimulating effects of Mouse sarcomas on the sensory and sympathetic nervous system of chick embryos. J Exp Zool 1951; 116; 321-62.
  37. Lu B. Pang PT, Woo NH. The yin and yang of neurotrophin action. Nat Rev Neurosci 2005; 6; 603-14.
  38. Lucas JH, Gross GW, Emery DG, Gardner CR. Neuronal survival or death after dentrite transection close to the perikaryon: correlation with electrophisiologic, morphologic and ultrastructural changes. Cent Nerv Syst Trauma 1985; 2; 231-55.
  39. Ma YT, Hsieh T, Forbes M.E, Johnson JE, Frost DO. BDNF injected into the superior colliculus reduces developmental retinal ganglion cell death. J Neurosci 1988; 18; 2097-107.
  40. Markus A, Patel TD, Snider WD. Neurotrophic factors and axonal growth. Current Opinion in Neurobiology 2002; 12; 523-31.
  41. Miralles F, Philippe P, Czernichow P, Scharfmann R. Expression of nerve growth factor and its high-affinity receptor Trk-Ain the rat pancreas during embryonic and fetal life. J Endocrinol 1998; 156; 431–39.
  42. Oberg-Welsh C. and Welsh M. Effects of certain growth factors on in vitro maturation of rat fetal islet-like structures. Pancreas 1996; 12; 334–39.
  43. Otto D, Unsicker K, Grothe C. Pharmacological effects of nerve growth factor and fibroblast growth factor applied to the transectioned sciatic nerve on neuron death in adult rat dorsal root ganglia. Neurosci Lett 1987; 83; 156-60.
  44. í–ztürk G. and Erdoğan E. Multidimensional long-term time lapse microscopy of in vitro peripheral nerve regeneration. Microscopy Reseach and Technique 2004; 64; 228-42.
  45. í–ztürk G, Cengiz N, Erdoğan E, Him A, Kaval Oğuz E, Yenidünya E, Ayşit N. Two Distinct Types of Dying Back Axonal Degeneration in vitro. Neuropathology and Applied Neurobiology 2013; 39; 362-76.
  46. Park H. and Poo M. Neurotrophin regulation of neural circuit development and function. Nature Reviews/Neuroscience 2013; 14; 7-23.
  47. Petruska JC, Ichiyama RM, Jindrich DL, Crown ED, Tansey KE, Roy RR, Edgerton VR, Mendell LM. Changes in motoneuron properties and synaptic inputs releated to step training after spinal cord transection in rats. J Neurosci 2007; 27; 4460-71.
  48. Rich KM, Luszczynski JR, Osborne PA, Johnson EMJr. Nerve growth factor protects adult sensory neurons from cell death and atrophy caused by nerve injury. J Neurocytol 1987; 16; 261-68.
  49. Rosser A. and Svendsen CN. Stem cells for cell replacement therapy: a therapeutic strategy for HD?. Mov Disord 2014; 29; 1446–54.
  50. Sato A, Okumura K, Matsumoto S, Hattori K, Hattori S, Shinohara M, Endo F. Isolation, tissue localization, and cellular characterization of progenitors derived from adult human salivary glands. Cloning Stem Cells 2007; 9; 191–205.
  51. Schlaepfer WW. and Bunge RP. Effects of calcium ion concentration on the degeneration of amputated axons in tissue culture. J Cell Biol 1973; 59; 456–70.
  52. Sievers C, Platt N, Perry VH, Coleman MP, Conforti L. Neurites undergoing Wallerian degeneration show an apoptotic-like process with Annexin V positive staining and loss of mitochondrial membrane potential. Neurosci Res 2003; 46; 161–69.
  53. Smeyne RJ, Klein R, Schnapp A, Long LK, Bryant S, Lewin A, Lira SA, Barbacid M. Severe sensory and sympathetic neuropathies in mice carrying a disrupted Trk/NGF receptor gene. Nature 1994; 368; 246-49.
  54. Song XY, Li F, Zhang FH, Zhong JH, Zhou XF. Peripherally-derived BDNF promotes regeneration of ascending sensory neurons after spinal cord injury. Plos One 2008; 3(3); e1707.
  55. Taylor AN, Rahman SU, Tio DL, Sanders MJ, Bando JK, Truong AH, Prolo P. Lasting neuroendocrine-immune effects of traumatic brain injury in rats. J Neurotrauma 2006; 23; 1802-13.
  56. Vejsada R, Tseng JL, Lindsay RM, Acheson A, Aebischer P, Kato AC. Synergistic but transient rescue effects of BDNF and GDNF on axotomized neonatal motoneurons. Neurosci. 1988; 84; 129-39.
  57. Xiao N. and Le QT. Neurotrophic factors and their potential applications in tissue regeneration. Arch Immunol Ther Exp 2015; 64(2); 89-99.
  58. Yawo H. and Kuno M. How a nerve ï¬ber repairs its cut end: involvement of phospholipase A2. Science 1983; 222; 1351–53.
  59. Yip HK, Rich KM, Lampe PA, Johnson EMJr. The effects of nerve growth factor and its antiserum on the postnatal development and survival after injury of sensory neurons in rat dorsal root ganglia. J Neurosci 1984; 4; 2986-92.
  60. Zheng F, Zhou X, Moon C, Wang H. Regulation of brain-derved neurotrphic factor expession in neurons. Int J Physiol Pharmacol 2012; 4;188-200.
  61. Zuccato C. and Cattaneo E. Role of brain-derived neurotrophic factor in Huntington's disease. Progress in neurobiology 2007; 81; 294-330.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 777 Download : 424