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Vol. 64 No. 11: Issue 11

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Screening for novel peptides specifically binding to the surface of ectopic endometrium cells by phage display

https://doi.org/10.14715/cmb/2018.64.11.7
Yan Linzhi
Department of Gynecology and Obstetrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, P.R. China
Cao Dan
Department of Gynecology and Obstetrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, P.R. China
Samuel Kofi Arhin
Department of Gynecology and Obstetrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, P.R. China
Wang Ledan
Department of Gynecology and Obstetrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, P.R. China
Li Wenju
Department of Gynecology and Obstetrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, P.R. China
Lv Jieqiang
Department of Gynecology and Obstetrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, P.R. China

Corresponding Author(s) : Lv Jieqiang

jieqianglu@126.com

Cellular and Molecular Biology, Vol. 64 No. 11: Issue 11

Abstract

A 7-mer phage display library was employed to isolate novel peptides that specifically bind to ectopic endometrium in vitro. Phage display technology with biopanning and rapid analysis of selective interactive ligands between ectopic and eutopic endometrium cells was utilized. After 5 rounds of biopanning, 50 phage clones were randomly selected and analyzed by enzyme-linked immunosorbent assay and DNA sequencing. A peptide-competitive inhibition assay was performed to identify the affinity of positive phages toward ectopic endometrium cells. The most enriched polypeptide RTRLHTR showed higher affinity toward ectopic endometrium cells.The polypeptide RTRLHTR screened by phage display technology may offer a new direction for early diagnosis and treatment of endometriosis.


 

Keywords

Ectopic endometrium cells Phage display technology Phage peptide library Peptides.
Linzhi, Y., Dan, C., Arhin, S. K., Ledan, W., Wenju, L., & Jieqiang, L. (2018). Screening for novel peptides specifically binding to the surface of ectopic endometrium cells by phage display. Cellular and Molecular Biology, 64(11), 36–40. https://doi.org/10.14715/cmb/2018.64.11.7
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