Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis

Zhang Siwei; Wu Zhen; Zhou Zhi; Hu Xuguang; Li Yousheng

doi:10.14715/cmb/2019.65.7.13

Issue

Vol. 65 No. 7: Issue 7

Issue Published : October 14, 2019

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Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis

https://doi.org/10.14715/cmb/2019.65.7.13

Zhang Siwei

Department of Traditional Chinese Medicine, Shenzhen People's Hospital (Second Clinical Medical College of Jinan University, First Affiliated Hospital of Southern University of Science and Technology), NO. 1017 Dongmen North Road, Luohu District, Shenzhen, Guangdong Province, China, 518020

Wu Zhen

School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, NO. 280 Daxuecheng Outer Ring East Road, Guangzhou, Guangdong Province, China, 510006

Zhou Zhi

Microbiology Laboratory, Shenzhen Institute for Drug Control, No. 28 Gaoxin Middle Second Road, Nanshan District, Shenzhen City, Guangdong Province, China, 518057

Hu Xuguang

School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, NO. 280 Daxuecheng Outer Ring East Road, Guangzhou, Guangdong Province, China, 510006

Li Yousheng

Corresponding Author(s) : Li Yousheng

ymdf83@163.com

Cellular and Molecular Biology, Vol. 65 No. 7: Issue 7
Article Published : September 30, 2019

Abstract

This study was aimed to investigate the anti-tumor activity of rubimaillin in vitro, and the mechanism involved. The inhibitory effect of rubimaillin on cell proliferation was determined with MTT assay. Apoptosis was assayed using AV/PI double staining, while the mitochondrial membrane potential of SKOV-3 cells was determined with Rhodamine 123 (Rh123) staining. Western blot assay was used to determine the effect of rubimaillin on the expressions of Bcl-2, Bax, PARP, cleaved PARP, caspase3, cleaved caspase3, and other apoptosis-related proteins in SKOV-3 cells. Rubimaillin inhibited the growth of SKOV-3 cells in a concentration-dependent manner and induced apoptosis of tumor cells through the mitochondrial apoptosis pathway. These results indicate that rubimaillin is a potential anti-ovarian cancer drug.

Keywords

Rubimaillin Ovarian cancer Mitochondria-dependent apoptosis.

Siwei, Z., Zhen, W., Zhi, Z., Xuguang, H., & Yousheng, L. (2019). Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis. Cellular and Molecular Biology, 65(7), 72–76. https://doi.org/10.14715/cmb/2019.65.7.13

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References

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Kehoe S. Screening for ovarian cancer. Maturitas 2015; 81: 423-424.
Bouwman P, Jonkers J. The effects of deregulated DNA damage signalling on cancer chemotherapy response and resistance. Nature reviews. Cancer 2012; 12: 587-598.
Krueger SA, Wilson GD. Flow cytometric DNA analysis of human cancers and cell lines. Methods Mol Med 2011; 731: 359-370.
Jager I, Hafner C, Welsch C, Schneider K, Iznaguen H, Westendorf J. The mutagenic potential of madder root in dyeing processes in the textile industry. Mutat Res 2006; 605: 22-29.
HIDEJI ITOKWA KM, KOICHI TAKEYA. Studies on a novel Anthraquinone and Its Glycosides isolated from Rubia cordifolia and R.akane. Chem Pharm Bull 1982; 31: 2353-2358.
Kuo SC, Chen PR, Lee SW, Chen ZT. Constituents of Roots of Rubia Lanceolata Hayata. J Chin Chem Soc 1995; 42; 869-871.
Itokawa H, Ibraheim ZZ, Qiao YF, Takeya K. Anthraquinones, naphthohydroquinones and naphthohydroquinone dimers from Rubia cordifolia and their cytotoxic activity. Chem Pharm Bull 1993; 41; 1869-1872.
Kawasaki Y, Goda Y, Yoshihira K. The mutagenic constituents of Rubia tinctorum. Chem Pharm Bull 1992; 40; 1504-1509.
Marec F, Kollarova I, Jegorov A. Mutagenicity of natural anthraquinones from Rubia tinctorum in the Drosophila wing spot test. Planta Med 2001; 67; 127-131.
Idhayadhulla A, Xia L, Lee YR, Kim SH, Wee YJ, Lee CS. Synthesis of novel and diverse mollugin analogues and their antibacterial and antioxidant activities. Bioorg Chem 2014; 52: 77-82.
Van MJ, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay. 2011; 88: 237-245.
Baracca A, Sgarbi G, Solaini G, Lenaz G. Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis. Biochim Biophys Acta 2003; 1606: 137-146.
Oztürk N, Korkmaz S, Oztürk Y, Başer KH. Effects of gentiopicroside, sweroside and swertiamarine, secoiridoids from gentian (Gentiana lutea ssp. symphyandra), on cultured chicken embryonic fibroblasts. Planta Med 2006; 72: 289-294.
Kim PK, Annis MG, Dlugosz PJ, Leber B, Andrews DW. During Apoptosis Bcl-2 Changes Membrane Topology at Both the Endoplasmic Reticulum and Mitochondria. Mol Cell 2004; 14: 523-529.
Engel M, Sidwell T, Vasanthakumar A, Grigoriadis G, Banerjee A. Thymic regulatory T cell development: role of signalling pathways and transcription factors. Clin Dev Immunol 2013; 2013: 617595.
Ricci JE, Waterhouse N, Green DR. Mitochondrial functions during cell death, a complex (I-V) dilemma. Cell Death Differ 2003; 10: 488-492.
Gahl RF, Dwivedi P, Tjandra N. Disease, Bcl-2 proteins bid and bax form a network to permeabilize the mitochondria at the onset of apoptosis. Cell Death Dis 2016; 7: 2424.
Lin HI, Lee YJ, Chen BF, Tsai MC, Lu JL, Chou CJ, et al. Involvement of Bcl-2 family, cytochrome c and caspase 3 in induction of apoptosis by beauvericin in human non-small cell lung cancer cells. Cancer Lett 2005; 230; 248-259.
Ly JD, Grubb DR, Lawen A. The mitochondrial membrane potential (Î”Ïˆm) in apoptosis; an update. Apoptosis 2003; 8: 115-128.

References

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018. CA Cancer J Clin 2018; 68: 7-30.

Kehoe S. Screening for ovarian cancer. Maturitas 2015; 81: 423-424.

Bouwman P, Jonkers J. The effects of deregulated DNA damage signalling on cancer chemotherapy response and resistance. Nature reviews. Cancer 2012; 12: 587-598.

Krueger SA, Wilson GD. Flow cytometric DNA analysis of human cancers and cell lines. Methods Mol Med 2011; 731: 359-370.

Jager I, Hafner C, Welsch C, Schneider K, Iznaguen H, Westendorf J. The mutagenic potential of madder root in dyeing processes in the textile industry. Mutat Res 2006; 605: 22-29.

HIDEJI ITOKWA KM, KOICHI TAKEYA. Studies on a novel Anthraquinone and Its Glycosides isolated from Rubia cordifolia and R.akane. Chem Pharm Bull 1982; 31: 2353-2358.

Kuo SC, Chen PR, Lee SW, Chen ZT. Constituents of Roots of Rubia Lanceolata Hayata. J Chin Chem Soc 1995; 42; 869-871.

Itokawa H, Ibraheim ZZ, Qiao YF, Takeya K. Anthraquinones, naphthohydroquinones and naphthohydroquinone dimers from Rubia cordifolia and their cytotoxic activity. Chem Pharm Bull 1993; 41; 1869-1872.

Kawasaki Y, Goda Y, Yoshihira K. The mutagenic constituents of Rubia tinctorum. Chem Pharm Bull 1992; 40; 1504-1509.

Marec F, Kollarova I, Jegorov A. Mutagenicity of natural anthraquinones from Rubia tinctorum in the Drosophila wing spot test. Planta Med 2001; 67; 127-131.

Idhayadhulla A, Xia L, Lee YR, Kim SH, Wee YJ, Lee CS. Synthesis of novel and diverse mollugin analogues and their antibacterial and antioxidant activities. Bioorg Chem 2014; 52: 77-82.

Van MJ, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay. 2011; 88: 237-245.

Baracca A, Sgarbi G, Solaini G, Lenaz G. Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis. Biochim Biophys Acta 2003; 1606: 137-146.

Oztürk N, Korkmaz S, Oztürk Y, Başer KH. Effects of gentiopicroside, sweroside and swertiamarine, secoiridoids from gentian (Gentiana lutea ssp. symphyandra), on cultured chicken embryonic fibroblasts. Planta Med 2006; 72: 289-294.

Kim PK, Annis MG, Dlugosz PJ, Leber B, Andrews DW. During Apoptosis Bcl-2 Changes Membrane Topology at Both the Endoplasmic Reticulum and Mitochondria. Mol Cell 2004; 14: 523-529.

Engel M, Sidwell T, Vasanthakumar A, Grigoriadis G, Banerjee A. Thymic regulatory T cell development: role of signalling pathways and transcription factors. Clin Dev Immunol 2013; 2013: 617595.

Ricci JE, Waterhouse N, Green DR. Mitochondrial functions during cell death, a complex (I-V) dilemma. Cell Death Differ 2003; 10: 488-492.

Gahl RF, Dwivedi P, Tjandra N. Disease, Bcl-2 proteins bid and bax form a network to permeabilize the mitochondria at the onset of apoptosis. Cell Death Dis 2016; 7: 2424.

Lin HI, Lee YJ, Chen BF, Tsai MC, Lu JL, Chou CJ, et al. Involvement of Bcl-2 family, cytochrome c and caspase 3 in induction of apoptosis by beauvericin in human non-small cell lung cancer cells. Cancer Lett 2005; 230; 248-259.

Ly JD, Grubb DR, Lawen A. The mitochondrial membrane potential (Î”Ïˆm) in apoptosis; an update. Apoptosis 2003; 8: 115-128.

Author biographies is not available.