Issue

Vol. 66 No. 1: Issue 1

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Oxidative stress and apoptosis in electromagnetic waves exposed Zebrafish embryos and protective effects of conductive nonwoven fabric

https://doi.org/10.14715/cmb/2019.66.1.12
íœnsal Veli íœstündağ
Department of Biochemistry, Faculty of Medicine, Medipol University, Kavacık, Istanbul, Turkey
Mustafa Sabri í–zen
Department of Textile Engineering, Faculty of Technology, Marmara University, Goztepe, Istanbul, Turkey
ć°smail íœnal
Department of Biochemistry, Faculty of Dentistry, Marmara University, Maltepe, Istanbul, Turkey
Perihan Seda Ateş
Department of Biochemistry, Faculty of Dentistry, Marmara University, Maltepe, Istanbul, Turkey
Ahmet Ata Alturfan
Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
Mehmet Akalın
Department of Textile Engineering, Faculty of Technology, Marmara University, Goztepe, Istanbul, Turkey
Erhan Sancak
Department of Textile Engineering, Faculty of Technology, Marmara University, Goztepe, Istanbul, Turkey
Ebru Emekli-Alturfan
Department of Biochemistry, Faculty of Dentistry, Marmara University, Maltepe, Istanbul, Turkey

Corresponding Author(s) : Ebru Emekli-Alturfan

ebruemekli@yahoo.com

Cellular and Molecular Biology, Vol. 66 No. 1: Issue 1

Abstract

The amount of technological products including television, radio transmitters, and mobile phone that have entered our daily life has increased in recent years. But these devices may cause adverse effects on human health. Electromagnetic shielding fabrics may limit and inhibit electromagnetic waves. Aim of our study was to evaluate electromagnetic wave blocking performance of nonwoven textile surfaces on zebrafish embryos that were exposed to electromagnetic waves at specific frequencies. Oxidant-antioxidant system parameters were evaluated spectrophotometrically. The expressions of tp53 and casp3a were evaluated by RT-PCR. Results showed that electromagnetic shielding fabrics produced as conductive nonwoven textile surfaces improved oxidant-antioxidant status and tp53 expression that were impaired in electromagnetic waves exposed zebrafish embryos. Also, electromagnetic shielding fabrics decreased casp3a expression responsible for the execution phase of apoptosis that increased in electromagnetic waves exposed zebrafish embryos.

 

Keywords

Electromagnetic wave Conductive fibres Zebrafish embryo Apoptosis Oxidant-antioxidant parameters.
íœstündağ, íœnsal V., í–zen, M. S., íœnal, ć°smail, Ateş, P. S., Alturfan, A. A., Akalın, M., Sancak, E., & Emekli-Alturfan, E. (2020). Oxidative stress and apoptosis in electromagnetic waves exposed Zebrafish embryos and protective effects of conductive nonwoven fabric. Cellular and Molecular Biology, 66(1), 70–75. https://doi.org/10.14715/cmb/2019.66.1.12
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. -Duhaini I. The effects of electromagnetic fields on human health. Physica Medica 2006; 32(3): 213.
  2. -Kaplan S. Electromagnetic field exposure and health: Microscopic, radiological and stereological studies. J Microsc Ultrastruct 2017; 5(4):ii-iii.
  3. -Ozen MS, Sancak E, Soin N, Shah TH, Siores E. Investigation of electromagnetic shielding effectiveness of needle punched nonwoven fabric produced from conductive silver coated staple polyamide fibre. Text Inst J 2016; 107(7): 912-922.
  4. -Damiano NW, Yan L, Whisner B, Zhou C. Simulation and Measurement of Through-the-Earth, Extremely Low-Frequency Signals Using Copper-Clad Steel Ground Rods. IEEE Trans Ind Appl 2017; 53(5): 5088.
  5. -Moulder, J.E., Foster, K.R., Erdreich, L.S., McNamee, J.P. Mobile phones, mobile phone base stations and cancer: a review. Int J Radiat Biol 2005; 81(3),189-203.
  6. -Senturk E, Tugrul S, Doğan R, Eren SB, Ozturan O, Koçyiğit A, et al. Effect of Radiofrequency on DNA Damage and Oxidative Status in Patients with Turbinate Hypertrophy. Indian J Otolaryngol Head Neck Surg 2017; 1-6.
  7. -Chen HC, Lee KC, Lin JH. Electromagnetic and electrostatic shielding properties of co-weaving-knitting fabrics reinforced composites. J Comp A Appl Sci Eng 2004;35: 1249-1256.
  8. -Ceken F, Kayacan í–, í–zkurt A, Uğurlu SS. The electromagnetic shielding properties of some conductive knitted fabrics produced on single or double needle bed of a flat knitting machine. J Text I 2012; 9:103.
  9. -Mudbhary R & Sadler KC. Epigenetics, development, and cancer: zebrafish make their mark. Birth Defects Res C Embryo Today 2011; 93(2):194-203.
  10. -Sancak E, Akalin M, Usta M, Yüksek M, í–zen MS. The Effects of Fabric and Conductive Wire Properties on Electromagnetic Shielding Effectiveness and Surface Resistivity of Interlock Knitted Fabrics. Fibers&Polymers 2018; 4: 843-853.
  11. -íœstündağ UV, íœnal ć°, Ateş PS, Alturfan AA, Yiğitbaşı T, Emekli-Alturfan E. Bisphenol A and di(2-ethylhexyl) phthalate exert divergent effects on apoptosis and the Wnt/β-catenin pathway in zebrafish embryos: A possible mechanism of endocrine disrupting chemical action. Toxicol Ind Health 2017; 33(12): 901.
  12. -Westerfield M. The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish, University of Oregon Press, Eugene, OR. 1995.
  13. -Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193;265-275.
  14. -Yagi K. Assay for blood plasma or serum. Method Enzymol, 1984;105, 328-331.
  15. -Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001; 5: 62-71.
  16. -Mylorie AA, Collins H, Umbles C, Kyle J. Erythrocyte superoxide dismutase activity and other parameters of cupper status in rats ingesting lead acetate. Toxicol Appl Pharmacol 1986; 82;512-520A.
  17. -Habig WH, Jacoby WB. Assays for differentation of glutathion- s- transferases. Methods Enzymol 1981; 77:398-405.
  18. -Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 2001; 25:402-408.
  19. -Caballero MV, Candiracci M. Zebrafish as screening model for detecting toxicity and drugs efficacy. J Unexplored Med Data 2018; 3:4.
  20. -Kim YM, Lim HM, Ro HS. Pulsed Electromagnetic Fields Increase Pigmentation through the p-ERK/p-p38 Pathway in Zebrafish (Danio rerio). Int J Mol Sci 2018; 19;3211.
  21. -Harkless R, Al-Quraishi M, Vagula, MC. Radiation hazards of radio frequency waves on the early embryonic development of Zebrafish. Proc. SPIE 2014;9112.
  22. -Fragopoulou AF, Koussoulakos SL, Margaritis LH. Cranial and postcranial skeletal variations induced in mouse embryos by mobile phone radiation. Pathophysiology 2010; 7(3):169-77.
  23. -Kıvrak EG, Yurt KK, Kaplan AA, Alkan I, Altun G. Effects of electromagnetic fields exposure on the antioxidant defense system. J Microsc Ultrastruct 2017; 5(4):167–176.
  24. -Halliwell B. Oxidative stress and cancer: have we moved forward? Biochem J 2007; 1:401(1);1-11.
  25. -Ozguner F, Oktem F, Ayata A, Koyu A, Yılmaz HR. A novel antioxidant agent caffeic acid phenethyl ester prevents long-term mobile phone exposure-induced renal impairment in rat. Prognostic value of malondialdehyde, N-acetyl-beta-D-glucosaminidase and nitric oxide determination. Mol Cell Biochem 2005; 277(1-2):73-80.
  26. -Martí­nez-Sámano J, Torres-Durán PV, Juárez-Oropeza MA, Verdugo-Diaz L. Effect of acute extremely low frequency electromagnetic field exposure on the antioxidant status and lipid levels in rat brain. Arch Med Res 2012; 43(3):183-9.
  27. -Ghanbari AA, Shabani K, Mohammad ND. Protective Effects of Vitamin E Consumption against 3MT Electromagnetic Field Effects on Oxidative Parameters in Substantia Nigra in Rats. Basic Clin Neurosci 2016;7(4):315-322.
  28. -Carr AM. Cell cycle. Piecing together the p53 puzzle. Science 2000; 10;287(5459):1765-6.
  29. -Porter, A.G., & Jänicke, R.U. Emerging roles of caspase-3 in apoptosis. Cell Death Differ 1999; 6(2): 99-104.
  30. -Liu YX, Tai JL, Li GQ, Zhang ZW, Xue JH, Liu H, et al. Exposure to 1950-MHz TD-SCDMA electromagnetic fields affects the apoptosis of astrocytes via caspase-3-dependent pathway. PLoS One 2012; 7: e42332.
  31. -Han Q, Chen R, Wang F, Chen S, Sun X, Guan, X, et al. Pre-exposure to 50 Hz-electromagnetic fields enhanced the antiproliferative efficacy of 5-fluorouracil in breast cancer MCF-7 cells. PLoS ONE 2018; 13(4): e0192888.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 1048 Download : 455