Decursin inhibits the oxidation of low-density lipoprotein and protects human aortic endothelial cells against oxidative damage

Xueting Wang; Jing Yu; Binlong Ji; Yuling Yang

doi:10.14715/cmb/2020.66.3.24

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Vol. 66 No. 3: Issue 3

Issue Published : June 8, 2020

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Decursin inhibits the oxidation of low-density lipoprotein and protects human aortic endothelial cells against oxidative damage

https://doi.org/10.14715/cmb/2020.66.3.24

Xueting Wang

Department of International medical, The First Affiliated Hospital of Harbin Medical University, Harbin, PR China

Jing Yu

Department of International medical, The First Affiliated Hospital of Harbin Medical University, Harbin, PR China

Binlong Ji

Department of Emergency Medicine, the 401st Hospital of PLA, Qingdao, PR China

Yuling Yang

Department of International medical, The First Affiliated Hospital of Harbin Medical University, Harbin, PR China

Corresponding Author(s) : Yuling Yang

tkgv14@163.com

Cellular and Molecular Biology, Vol. 66 No. 3: Issue 3
Article Published : June 5, 2020

Abstract

Atherosclerosis is the pathological basis of cardiovascular diseases (CVDs) which are the leading cause of death worldwide. The pathogenesis of atherosclerosis itself is complex. Low-density lipoprotein (LDL) oxidation has been shown to increase lipid peroxide levels in arterial wall of atherosclerosis lesion site. Decursin is a coumarin with a range of pharmacological effects. The present study investigated the inhibitory effect of decursin on LDL oxidation, and its protective effect against oxidative damage in human aortic endothelial cells (HAECs). Two models of oxidative damage were used in this study: Cu2+-induced LDL oxidative damage and 2,2'-azobis-2-methyl-propanimidyl, dihydrochloride (AAPH)-induced oxidative damage of HAECs. The inhibitory effect of decursin on LDL oxidation, and its protective effect on oxidative damage of HAECs were determined. The results showed that the level of thiobarbituric acid reactive substances (TBARS) was significantly increased by Cu2+, but was significantly and concentration-dependently reduced after treatment with decursin (p < 0.05). There were only a few viable cells in AAPH-treated group, but treatment with decursin led to significant, time- and concentration-dependent increases in their viability (p < 0.05). The AAPH-induced oxidative damage significantly increased the activity of lactate dehydrogenase (LDH) and level of reactive oxygen species (ROS), but significantly reduced the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) (p < 0.05). However, treatment of HAECs with decursin significantly and concentration-dependently reduced the LDH activity and level of ROS, but significantly increased the activities of SOD and GPx, concentration-dependently (p < 0.05). It also significantly and concentration-dependently reduced apoptosis induced by AAPH (p < 0.05). These results show that decursin effectively inhibits LDL oxidation induced by Cu2+, and protects HAECs from oxidative damage caused by AAPH in vitro via a mechanism involving activation of SOD and GPx.

Keywords

Decursin Oxidative damage Low-density lipoprotein Aortic endothelial cells Antioxidant enzyme.

Wang, X., Yu, J., Ji, B., & Yang, Y. (2020). Decursin inhibits the oxidation of low-density lipoprotein and protects human aortic endothelial cells against oxidative damage. Cellular and Molecular Biology, 66(3), 155–158. https://doi.org/10.14715/cmb/2020.66.3.24

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References

Manson JE, Cook NR, Lee IM, Christen W, Bassuk SS, Mora S, et al. Vitamin D supplements and prevention of cancer and cardiovascular disease. N Engl J Med 2019; 380: 33-44.
Manson JE, Cook NR, Lee IM, Christen W, Bassuk SS, Mora S, et al. Marine n-3 fatty acids and prevention of cardiovascular disease and cancer. N Engl J Med 2019; 380: 23-32.
Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 1993; 362: 801.
Kita T, Ishii K, Yokode M, Kume N, Nagano Y, Arai H, et al. The role of oxidized low density lipoprotein in the pathogenesis of atherosclerosis. Eur Heart J 1990; 11: 122-127.
Ahn KS, Sim WS, Kim IH. Decursin: a cytotoxic agent and protein kinase C activator from the root of Angelica gigas. Planta Med 1996; 62: 7-9.
Kang S, Lee K, Sung S, Kim Y. Coumarins isolated from Angelica gigas inhibit acetylcholinesterase: structure-activity relationships. J Nat Prod 2000; 49: 226-227.
Herath W, Reddy N, Khan I. Microbial metabolism. Part 8. The pyranocoumarin, decursin. Chem Pharm Bull (Tokyo) 2007; 55: 1512-1513.
Lee S, Lee YS, Jung SH, Shin KH, Kim BK, Kang SS. Anti-tumor activities of decursinol angelate and decursin from Angelica gigas. Arch Pharm Res 2003; 26: 727-730.
Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15: 7313-7352.
Hwang JT, Kim SH, Hur HJ, Kim HJ, Park JH, Sung MJ, et al. Decursin, an active compound isolated from Angelica gigas, inhibits fat accumulation, reduces adipocytokine secretion and improves glucose tolerance in mice fed a high-fat diet. Phytother Res 2012; 26: 633-638.
Thilakarathna SH, Rupasinghe HV, Needs PW. Apple peel bioactive rich extracts effectively inhibit in vitro human LDL cholesterol oxidation. Food Chem 2013; 138: 463-470.
Liu F, Ng T. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci 2000; 66: 725-735.
Libby PJ. Changing concepts of atherogenesis. J Intern Med 2000; 247: 349-358.
Chait A, Brazg RL, Tribble DL, Krauss RM. Susceptibility of small, dense, low-density lipoproteins to oxidative modification in subjects with the atherogenic lipoprotein phenotype, pattern B. Am J Med 1993; 94: 350-356.
World Health Organization (WHO). World health statistics 2016: monitoring health for the SDGs sustainable development goals. World Health Organization 2016; 41: 293-328.
Pan Y, Guo C, Ma X, Wang J, Liu X, Sun M, et al. The estrogen-like protective effect of ginsenoside Rb3 on oxidative stress and dysfunction of endothelial cells induced by oxidized low-density lipoprotein. Yao Xue Xue Bao 2014; 49: 1406-1412.
Yang TL, Lin FY, Chen YH, Chiu JJ, Shiao MS, Tsai CS, et al. Salvianolic acid B inhibits low-density lipoprotein oxidation and neointimal hyperplasia in endothelium-denuded hypercholesterolaemic rabbits. J Sci Food Agric 2011; 91: 134-141.
Kim BS, Seo H, Kim HJ, Bae SM, Son HN, Lee YJ, et al. Decursin from Angelica gigas Nakai inhibits B16F10 melanoma growth through induction of apoptosis. J Med Food 2015; 18: 1121-1127.
Ighodaro O, Akinloye OJ. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J Med 2018; 54: 287-293.
Fotakis G, Timbrell JA. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 2006; 160: 171-177.

References

Manson JE, Cook NR, Lee IM, Christen W, Bassuk SS, Mora S, et al. Vitamin D supplements and prevention of cancer and cardiovascular disease. N Engl J Med 2019; 380: 33-44.

Manson JE, Cook NR, Lee IM, Christen W, Bassuk SS, Mora S, et al. Marine n-3 fatty acids and prevention of cardiovascular disease and cancer. N Engl J Med 2019; 380: 23-32.

Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 1993; 362: 801.

Kita T, Ishii K, Yokode M, Kume N, Nagano Y, Arai H, et al. The role of oxidized low density lipoprotein in the pathogenesis of atherosclerosis. Eur Heart J 1990; 11: 122-127.

Ahn KS, Sim WS, Kim IH. Decursin: a cytotoxic agent and protein kinase C activator from the root of Angelica gigas. Planta Med 1996; 62: 7-9.

Kang S, Lee K, Sung S, Kim Y. Coumarins isolated from Angelica gigas inhibit acetylcholinesterase: structure-activity relationships. J Nat Prod 2000; 49: 226-227.

Herath W, Reddy N, Khan I. Microbial metabolism. Part 8. The pyranocoumarin, decursin. Chem Pharm Bull (Tokyo) 2007; 55: 1512-1513.

Lee S, Lee YS, Jung SH, Shin KH, Kim BK, Kang SS. Anti-tumor activities of decursinol angelate and decursin from Angelica gigas. Arch Pharm Res 2003; 26: 727-730.

Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15: 7313-7352.

Hwang JT, Kim SH, Hur HJ, Kim HJ, Park JH, Sung MJ, et al. Decursin, an active compound isolated from Angelica gigas, inhibits fat accumulation, reduces adipocytokine secretion and improves glucose tolerance in mice fed a high-fat diet. Phytother Res 2012; 26: 633-638.

Thilakarathna SH, Rupasinghe HV, Needs PW. Apple peel bioactive rich extracts effectively inhibit in vitro human LDL cholesterol oxidation. Food Chem 2013; 138: 463-470.

Liu F, Ng T. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci 2000; 66: 725-735.

Libby PJ. Changing concepts of atherogenesis. J Intern Med 2000; 247: 349-358.

Chait A, Brazg RL, Tribble DL, Krauss RM. Susceptibility of small, dense, low-density lipoproteins to oxidative modification in subjects with the atherogenic lipoprotein phenotype, pattern B. Am J Med 1993; 94: 350-356.

World Health Organization (WHO). World health statistics 2016: monitoring health for the SDGs sustainable development goals. World Health Organization 2016; 41: 293-328.

Pan Y, Guo C, Ma X, Wang J, Liu X, Sun M, et al. The estrogen-like protective effect of ginsenoside Rb3 on oxidative stress and dysfunction of endothelial cells induced by oxidized low-density lipoprotein. Yao Xue Xue Bao 2014; 49: 1406-1412.

Yang TL, Lin FY, Chen YH, Chiu JJ, Shiao MS, Tsai CS, et al. Salvianolic acid B inhibits low-density lipoprotein oxidation and neointimal hyperplasia in endothelium-denuded hypercholesterolaemic rabbits. J Sci Food Agric 2011; 91: 134-141.

Kim BS, Seo H, Kim HJ, Bae SM, Son HN, Lee YJ, et al. Decursin from Angelica gigas Nakai inhibits B16F10 melanoma growth through induction of apoptosis. J Med Food 2015; 18: 1121-1127.

Ighodaro O, Akinloye OJ. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J Med 2018; 54: 287-293.

Fotakis G, Timbrell JA. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 2006; 160: 171-177.

Author biographies is not available.