Analgesic action of Rubimaillin in vitro and in vivo

Wanli Yan; Zhonglei Jiang; Fengyun Fan

doi:10.14715/cmb/2020.66.3.27

Issue

Vol. 66 No. 3: Issue 3

Issue Published : June 8, 2020

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Analgesic action of Rubimaillin in vitro and in vivo

https://doi.org/10.14715/cmb/2020.66.3.27

Wanli Yan

Department of Rehabilitation, Yantai Hospital of Traditional Chinese Medicine, Yantai, PR China

Zhonglei Jiang

Department of Rehabilitation, Yantai Hospital of Traditional Chinese Medicine, Yantai, PR China

Fengyun Fan

Department of Rehabilitation, Yantai Hospital of Traditional Chinese Medicine, Yantai, PR China

Corresponding Author(s) : Fengyun Fan

qhsm14@163.com

Cellular and Molecular Biology, Vol. 66 No. 3: Issue 3
Article Published : June 5, 2020

Abstract

Pain, a common symptom in clinics, is a serious impediment to quality of life. The analgesic drugs presently in use have poor efficacy, and are associated with undesirable side effects. Rubimaillin (Rub) is a naphthoquinone compound extracted from Chinese herbal medicine, and it has various biological activities. In this study, the analgesic effect of Rub, and its mechanism of action were investigated using glacial acetic acid-induced mice writhing model and a mice model of neurogenic and inflammatory bipolar pain. Analgesic effects were measured in different experimental groups. In vitro, RAW 264.7 cells were used to investigate the release of nitric oxide (NO), iNOS and COX-2 protein in RAW 264.7 cells stimulated with lipopolysaccharide (LPS). The results revealed that Rub reduced the number of acetic acid-induced writhing in mice, inhibited formalin-induced biphasic pain response, and suppressed the production of NO in RAW 264.7 cells. The mechanisms involved in the analgesic and anti-inflammatory effects of rub may be related to the inhibition of cyclooxygenase-2 (COX-2), endogenous inflammatory mediators, and reduction in the content of pain-induced mediators.

Keywords

Rubimaillin Analgesic Inflammation PEG2.

Yan, W., Jiang, Z., & Fan, F. (2020). Analgesic action of Rubimaillin in vitro and in vivo. Cellular and Molecular Biology, 66(3), 171–175. https://doi.org/10.14715/cmb/2020.66.3.27

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References

Guildford BJ, Daly-Eichenhardt A, Hill B, Sanderson K, McCracken LM. Analgesic reduction during an interdisciplinary pain management programme: treatment effects and processes of change. Br J Pain 2018;12: 72-86.
Wiese AD, Griffin MR, Schaffner W, Stein CM, Greevy RA, Mitchel EF, et al. Opioid analgesic use and risk for invasive pneumococcal diseases: a nested case–control study. Ann Intern Med 2018; 168: 396-404.
Cairat M, Fournier A, Murphy N, Biessy C, Scalbert A, Rinaldi S, et al. Nonsteroidal anti"inflammatory drug use and breast cancer risk in a European prospective cohort study. Int J Cancer 2018; 143: 1688-1695.
Tan JY, Chen XQ, Kang BJ, Qin ZX, Chen JH, Hu RD, et al. Myricetin protects against lipopolysaccharide-induced disseminated intravascular coagulation by anti-inflammatory and anticoagulation effect. Asian Pac J Trop Med 2018; 11: 255.
Cai Y, Sun M, Xing J, Corke H. Antioxidant phenolic constituents in roots of Rheum officinale and Rubia cordifolia: structureâˆ’ radical scavenging activity relationships. J Agric Food Chem 2004; 52: 7884-7890.
Idhayadhulla A, Xia L, Lee YR, Kim SH, Wee YJ, Lee CS. Synthesis of novel and diverse mollugin analogues and their antibacterial and antioxidant activities. Bioorg Chem 2014; 52: 77-82.
Tse WP, Che CT, Liu K, Lin ZX. Evaluation of the anti-proliferative properties of selected psoriasis-treating Chinese medicines on cultured HaCaT cells. J Ethnopharmacol 2006; 108: 133-141.
Matsuda D, Ohshiro T, Ohba M, Jiang W, Hong B, Si S, et al. The molecular target of rubimaillin in the inhibition of lipid droplet accumulation in macrophages. Biol Pharm Bull 2009; 32: 1317-1320.
Lu Y, Liu R, Sun C, Pan Y. An effective high"speed countercurrent chromatographic method for preparative isolation and purification of mollugin directly from the ethanol extract of the Chinese medicinal plant Rubia cordifolia. J Sep Sci 2007; 30: 1313-1317.
Chen MJ, Liang T, Zhou KY. [Effect of proanthocyanidins on COX-2 enzyme activity and COX-2 mRNA /protein expression in LPS-induced RAW264.7 cells]. Yao Xue Xue Bao 2005; 40: 406.
Peng B, Liu XM, Wang LW. Survey on pharmacological methodology of sedative Chinese materia medica. Chin Tradit Herbal Drugs 2005; 36: 1257-1260.
Cao S, Li Y, Wang L, Cui J, Jia N, Li R, et al. Synergistic analgesic effect of propofol–alfentanil combination through detecting the inhibition of cAMP signal pathway. J Pharm Pharmacol 2016; 68: 1170-1176.
Xu Y, Ku B, Geng X, Yao H, Zhang Y, Han J, et al. Analgesic effects of tetrodotoxin combined with acetylsalicylic acid on acetic acid-induced abdominal constriction and formalin test in mice. J Chin Pharm Sci 2005; 14: 186.
Florentino IF, Silva DP, Galdino PM, Lino RC, Martins JL, Silva DM, et al. Antinociceptive and anti-inflammatory effects of Memora nodosa and allantoin in mice. J Ethnopharmacol 2016; 186: 298-304.
Prasad AS, Beck FW, Grabowski SM, Kaplan J, Mathog RH. Zinc deficiency: changes in cytokine production and T-cell subpopulations in patients with head and neck cancer and in noncancer subjects. Proc Assoc Am Physicians 1997; 109: 68-77.
Arnold RJ, Layton A, Massanar M. Cost impact of monitoring exhaled nitric oxide in asthma management. Allergy Asthma Proc 2018; 39: 338-344.
Jiang YX, Dai YY, Pan YF, Wu XM, Yang Y, Bian K, et al. Total flavonoids from Radix Glycyrrhiza exert anti-inflammatory and Antitumorigenic effects by inactivating iNOS signaling pathways. Evid Based Complement Alternat Med 2018; 2018: 22.
Loynes CA, Lee JA, Robertson AL, Steel MJ, Ellett F, Feng Y, et al. PGE2 production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolution in vivo. Sci Adv 2018; 4: 8320.
Aoki T, Narumiya SJA, Supplements A. Prostaglandins and chronic inflammation. Trends Pharmacol Sci 2012; 33: 304-311.
Streicher JM, Kamei K, Ishikawa T, Herschman H, Wang Y. Compensatory hypertrophy induced by ventricular cardiomyocyte-specific COX-2 expression in mice. J Mol Cell Cardiol 2010; 49: 88-94.

References

Guildford BJ, Daly-Eichenhardt A, Hill B, Sanderson K, McCracken LM. Analgesic reduction during an interdisciplinary pain management programme: treatment effects and processes of change. Br J Pain 2018;12: 72-86.

Wiese AD, Griffin MR, Schaffner W, Stein CM, Greevy RA, Mitchel EF, et al. Opioid analgesic use and risk for invasive pneumococcal diseases: a nested case–control study. Ann Intern Med 2018; 168: 396-404.

Cairat M, Fournier A, Murphy N, Biessy C, Scalbert A, Rinaldi S, et al. Nonsteroidal anti"inflammatory drug use and breast cancer risk in a European prospective cohort study. Int J Cancer 2018; 143: 1688-1695.

Tan JY, Chen XQ, Kang BJ, Qin ZX, Chen JH, Hu RD, et al. Myricetin protects against lipopolysaccharide-induced disseminated intravascular coagulation by anti-inflammatory and anticoagulation effect. Asian Pac J Trop Med 2018; 11: 255.

Cai Y, Sun M, Xing J, Corke H. Antioxidant phenolic constituents in roots of Rheum officinale and Rubia cordifolia: structureâˆ’ radical scavenging activity relationships. J Agric Food Chem 2004; 52: 7884-7890.

Idhayadhulla A, Xia L, Lee YR, Kim SH, Wee YJ, Lee CS. Synthesis of novel and diverse mollugin analogues and their antibacterial and antioxidant activities. Bioorg Chem 2014; 52: 77-82.

Tse WP, Che CT, Liu K, Lin ZX. Evaluation of the anti-proliferative properties of selected psoriasis-treating Chinese medicines on cultured HaCaT cells. J Ethnopharmacol 2006; 108: 133-141.

Matsuda D, Ohshiro T, Ohba M, Jiang W, Hong B, Si S, et al. The molecular target of rubimaillin in the inhibition of lipid droplet accumulation in macrophages. Biol Pharm Bull 2009; 32: 1317-1320.

Lu Y, Liu R, Sun C, Pan Y. An effective high"speed countercurrent chromatographic method for preparative isolation and purification of mollugin directly from the ethanol extract of the Chinese medicinal plant Rubia cordifolia. J Sep Sci 2007; 30: 1313-1317.

Chen MJ, Liang T, Zhou KY. [Effect of proanthocyanidins on COX-2 enzyme activity and COX-2 mRNA /protein expression in LPS-induced RAW264.7 cells]. Yao Xue Xue Bao 2005; 40: 406.

Peng B, Liu XM, Wang LW. Survey on pharmacological methodology of sedative Chinese materia medica. Chin Tradit Herbal Drugs 2005; 36: 1257-1260.

Cao S, Li Y, Wang L, Cui J, Jia N, Li R, et al. Synergistic analgesic effect of propofol–alfentanil combination through detecting the inhibition of cAMP signal pathway. J Pharm Pharmacol 2016; 68: 1170-1176.

Xu Y, Ku B, Geng X, Yao H, Zhang Y, Han J, et al. Analgesic effects of tetrodotoxin combined with acetylsalicylic acid on acetic acid-induced abdominal constriction and formalin test in mice. J Chin Pharm Sci 2005; 14: 186.

Florentino IF, Silva DP, Galdino PM, Lino RC, Martins JL, Silva DM, et al. Antinociceptive and anti-inflammatory effects of Memora nodosa and allantoin in mice. J Ethnopharmacol 2016; 186: 298-304.

Prasad AS, Beck FW, Grabowski SM, Kaplan J, Mathog RH. Zinc deficiency: changes in cytokine production and T-cell subpopulations in patients with head and neck cancer and in noncancer subjects. Proc Assoc Am Physicians 1997; 109: 68-77.

Arnold RJ, Layton A, Massanar M. Cost impact of monitoring exhaled nitric oxide in asthma management. Allergy Asthma Proc 2018; 39: 338-344.

Jiang YX, Dai YY, Pan YF, Wu XM, Yang Y, Bian K, et al. Total flavonoids from Radix Glycyrrhiza exert anti-inflammatory and Antitumorigenic effects by inactivating iNOS signaling pathways. Evid Based Complement Alternat Med 2018; 2018: 22.

Loynes CA, Lee JA, Robertson AL, Steel MJ, Ellett F, Feng Y, et al. PGE2 production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolution in vivo. Sci Adv 2018; 4: 8320.

Aoki T, Narumiya SJA, Supplements A. Prostaglandins and chronic inflammation. Trends Pharmacol Sci 2012; 33: 304-311.

Streicher JM, Kamei K, Ishikawa T, Herschman H, Wang Y. Compensatory hypertrophy induced by ventricular cardiomyocyte-specific COX-2 expression in mice. J Mol Cell Cardiol 2010; 49: 88-94.

Author biographies is not available.