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Vol. 66 No. 3: Issue 3

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Nitric oxide donor andrographolide enhances humoral and cell-mediated immune responses

https://doi.org/10.14715/cmb/2020.66.3.28
Bing Zhang
Department of Clinical Lab, The Central Hospital of Nanchong, Nanchong, PR China
Bo Yang
Department of Clinical Lab, The Central Hospital of Nanchong, Nanchong, PR China
Lijun Du
Department of Clinical Lab, The Central Hospital of Nanchong, Nanchong, PR China
Yunfen Guo
Department of Clinical Lab, The Central Hospital of Nanchong, Nanchong, PR China

Corresponding Author(s) : Bo Yang

xsidi9@163.com

Cellular and Molecular Biology, Vol. 66 No. 3: Issue 3

Abstract

The present study was aimed to investigate the regulatory effect of Nitric oxide  donor andrographolide (Q-1) on cellular immunity in patients with chronic hepatitis B. Peripheral blood mononuclear cells (PBMCs) were isolated from patients with chronic hepatitis B. Cell viability was assessed using 3"‘(4,5"‘dimethyl"‘thiazol"‘2"‘yl)"‘2,5"‘diphenyl"‘2H"‘tetrazolium bromide (MTT) assay. The levels of expression of interferon gamma (IFN-γ), interleukin 4 (IL-4), interleukin 10 (IL-10) and tumor necrosis factor α (TFN-α) in PBMCs of patients with chronic hepatitis B were determined using real-time quantitative polymerase chain reaction (qRT-PCR). Anti-HBV effect of isolated HBV DNA was also assessed in vitro. Q-1 had no significant effect on the viability of Vero and isolated PBMCs (p > 0.05). The expression of IFN-γ in PBMCs of control patients significantly and time-dependently increased after treatment with Q-1, but the expressions of IL-4 and IL-10 in PBMCs of patients with chronic hepatitis B were decreased significantly and time-dependently (p < 0.05). The function of Th1 cells was significantly enhanced by Q-1 treatment (p < 0.05). The mean replication of HBV DNA in HepG2cells at the three concentrations of Q-1 and adefovir were 3.96 í— 106, 4.13 í— 106 and 4.53 í— 106 copies/mL, respectively. There was no significant difference in the expression of HBV DNA among the concentration levels. These results indicate that andrographolide enhances the function of HBV-specific T cells in patients with chronic hepatitis B.

Keywords

Chronic hepatitis B Andrographolide Cytotoxic T lymphocytes Cellular immunity Expression.
Zhang, B., Yang, B., Du, L., & Guo, Y. (2020). Nitric oxide donor andrographolide enhances humoral and cell-mediated immune responses. Cellular and Molecular Biology, 66(3), 176–180. https://doi.org/10.14715/cmb/2020.66.3.28
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