Issue

Vol. 66 No. 2: Issue 2

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Rubimaillin suppresses proliferation, migration and invasion of prostate cancer cells via the Notch-1/MMP signaling pathway

https://doi.org/10.14715/cmb/2020.66.2.21
Fangzhen Cai
Department of Urology Surgery, The Second Attached Hospital of Fujian Medical University, 34 North Zhongshan Road, Quanzhou City, Fujian Province, 362000, China
Shuxin Guo
Department of Urology Surgery, The Second Attached Hospital of Fujian Medical University, 34 North Zhongshan Road, Quanzhou City, Fujian Province, 362000, China
Sihuai Huang
Department of Urology Surgery, The Second Attached Hospital of Fujian Medical University, 34 North Zhongshan Road, Quanzhou City, Fujian Province, 362000, China
Jianwei Li
Department of Urology Surgery, The Second Attached Hospital of Fujian Medical University, 34 North Zhongshan Road, Quanzhou City, Fujian Province, 362000, China
Wenbin Liu
Department of Urology Surgery, The Second Attached Hospital of Fujian Medical University, 34 North Zhongshan Road, Quanzhou City, Fujian Province, 362000, China

Corresponding Author(s) : Wenbin Liu

amnak2@163.com

Cellular and Molecular Biology, Vol. 66 No. 2: Issue 2

Abstract

This study was aimed to explore the effect of Rubimaillin on the survival, migration, and invasion of prostate cancer cell lines DU145 and PC3, and its mechanism. CCK-8 method was used to detect the effects of different concentrations of rubs (0, 5, 10, 20, 40 and 80 μM) on the activity of DU145 cells and PC3 cells. Transwell cell lab test was used to detect the migration and invasion of cells. Western blot was used to detect Notch-1, MMP-2, MMP-9 and Hes-1 protein levels. The CCK-8 assay showed that Rub inhibited the activity of Du145 and PC3 cells in a concentration dependent manner. When the concentration reached 40 μM, the inhibition reached the maximum. After Rub intervention, the migration and invasion ability of Du145 and PC3 cells decreased significantly, while the expression levels of Notch-1, MMP-2, MMP-9 and Hes-1 protein decreased significantly. Rub can inhibit the growth, migration and invasion of prostate cancer cell line DU145 and PC3. The mechanism may be related to the inhibition of Notch-1, MMP-2, MMP-9 and Hes-1 by Rub.

Keywords

Rubimaillin Prostate cancer cells Cell migration Cell invasion Notch-1/MMP signaling pathway.
Cai, F., Guo, S., Huang, S., Li, J., & Liu, W. (2020). Rubimaillin suppresses proliferation, migration and invasion of prostate cancer cells via the Notch-1/MMP signaling pathway. Cellular and Molecular Biology, 66(2), 130–134. https://doi.org/10.14715/cmb/2020.66.2.21
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Ferlay J, Steliarova-Foucher E, Lortet-Tieulent J, Rosso S, Coebergh JW, Comber H, et al. Cancer incidence and mortality patterns in Europe: Estimates for 40 countries in 2012. Eur J Cancer 2013; 49: 1374-1403.
  2. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin 2015; 65: 5-29.
  3. Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, et al. Cancer statistics in China, 2015. CA Cancer J Clin 2016; 66: 115-132.
  4. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin 2019; 69: 7-34.
  5. Huggins C. EFFECT OF ORCHIECTOMY AND IRRADIATION ON CANCER OF THE PROSTATE. Ann Surg 1942; 115: 1192-1200.
  6. Segal RJ, Reid RD, Courneya KS, Malone SC, Parliament MB, Scott CG, et al. Resistance Exercise in Men Receiving Androgen Deprivation Therapy for Prostate Cancer. J Clin Oncol 2003; 21: 1653-1659.
  7. Harris WP, Mostaghel EA, Nelson PS, Montgomery B. Androgen deprivation therapy: progress in understanding mechanisms of resistance and optimizing androgen depletion. Nat Clin Pract Urol 2009; 6: 76-85.
  8. Pienta KJ, Smith DC. Advances in prostate cancer chemotherapy: a new era begins. CA Cancer J Clin 2005; 55: 300-318.
  9. Sweeney C, Chen Y, Carducci MA, Liu G, Jarrard DF, Eisenberger M, et al. Chemohormonal Therapy in Metastatic Hormone-Sensitive Prostate Cancer. N Engl J Med 2015; 373: 737-746.
  10. James ND, Sydes MR, Clarke NW, Mason MD, Dearnaley DP, Spears MR, et al. Addition of docetaxel, zoledronic acid, or both to first-line long-term hormone therapy in prostate cancer (STAMPEDE): survival results from an adaptive, multiarm, multistage, platform randomised controlled trial. Lancet 2016; 387: 1163-1177.
  11. Itokawa H, Mihara K, Takeya K. Studies on a novel anthraquinone and its glycosides isolated from Rubia cordifolia and R. akane. Chem Pharm Bull 1983; 31: 2353-2358.
  12. Kuo SC, Chen PR, Lee SW, Chen ZT. Constituents of roots of Rubia lanceolata Hayata. J Chin Chem Soc 1995; 42: 869-871.
  13. Jäger I, Hafner C, Welsch C, Schneider K, Iznaguen H, Westendorf J. The mutagenic potential of madder root in dyeing processes in the textile industry. Mutat Res 2006; 605: 22-29.
  14. Marec F, Kollárová I, Jegorov A. Mutagenicity of natural anthraquinones from Rubia tinctorum in the Drosophila wing spot test. Planta Med 2001; 67: 127-131.
  15. Idhayadhulla A, Xia L, Lee YR, Kim SH, Wee YJ, Lee CS. Synthesis of novel and diverse mollugin analogues and their antibacterial and antioxidant activities. Bioorg Chem 2014; 52: 77-82.
  16. Siwei Z, Zhen W, Zhi Z, Xuguang H, Yousheng L. Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis. Cell Mol Biol 2019; 65: 72-76.
  17. van Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay. Methods Mol Biol 2011; 88: 237-245.
  18. Wu CP, Ohnuma S, Ambudkar S. Discovering natural product modulators to overcome multidrug resistance in cancer chemotherapy. Curr Pharm Biotechnol 2011; 12: 609-620.
  19. Mann J. Natural products in cancer chemotherapy: past, present and future. Nat Rev Cancer 2002; 2: 143.
  20. Walker RA. The complexities of breast cancer desmoplasia. Breast Cancer Res 2001; 3: 143.
  21. Malemud CJ. Matrix metalloproteinases (MMPs) in health and disease: an overview. Front Biosci 2006; 11: 1696-1701.
  22. Roomi M, Monterrey J, Kalinovsky T, Rath M, Niedzwiecki A. Patterns of MMP-2 and MMP-9 expression in human cancer cell lines. Oncol Rep 2009; 21: 1323-1333.
  23. Bostwick DG, Wollan P, Adlakha K. Collagenous micronodules in prostate cancer. A specific but infrequent diagnostic finding. Arch Pathol Lab Med 1995; 119: 444-447.
  24. Júnior S, Matheus WE, Garcia PV, Stopiglia RM, Billis A, Ferreira U, et al. Characterization of reactive stroma in prostate cancer: involvement of growth factors, metalloproteinase matrix, sexual hormones receptors and prostatic stem cells. Int Braz J Urol 2015; 41: 849-858.
  25. Tomas D, Krušlin B. The potential value of (Myo) fibroblastic stromal reaction in the diagnosis of prostatic adenocarcinoma. Prostate 2004; 61: 324-331.
  26. Cox TR, Erler JT. Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and cancer. Dis Model Mech 2011; 4: 165-178.
  27. Oh J, Takahashi R, Kondo S, Mizoguchi A, Adachi E, Sasahara RM, et al. The membrane-anchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis. Cell 2001; 107: 789-800.
  28. Klein G, Vellenga E, Fraaije M, Kamps W, De Bont E. The possible role of matrix metalloproteinase (MMP)-2 and MMP-9 in cancer, eg acute leukemia. Crit Rev Oncol Hematol 2004; 50: 87-100.
  29. Turpeenniemi-Hujanen T. Gelatinases (MMP-2 and-9) and their natural inhibitors as prognostic indicators in solid cancers. Biochimie 2005; 87: 287-297.
  30. Rojiani MV, Alidina J, Esposito N, Rojiani AM. Expression of MMP-2 correlates with increased angiogenesis in CNS metastasis of lung carcinoma. Int J Clin Exp Pathol 2010; 3: 775.
  31. Brown GT, Murray GI. Current mechanistic insights into the roles of matrix metalloproteinases in tumour invasion and metastasis. J Pathol 2015; 237: 273-281.
  32. Mehlen P, Puisieux A. Metastasis: a question of life or death. Nat Rev Cancer 2006; 6: 449.
  33. Liotta LA. Tumor invasion and metastases: role of the basement membrane. Warner-Lambert Parke-Davis Award lecture. Am J Pathol 1984; 117: 339.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 883 Download : 459

Most read articles by the same author(s)