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Vol. 66 No. 2: Issue 2

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The application of DNA molecular markers in the study of Codonopsis species genetic variation, a review

https://doi.org/10.14715/cmb/2020.66.2.3
Mingjun Yang
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730000, China
Hosham Abdalrahman
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730000, China
Uwimbabazi Sonia
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730000, China
Alaa I Mohammed
Department of Haematology & Immunohaematology, Faculty of Medical Laboratories Sciences, University of Khartoum, Khartoum, Sudan
Uwitugabiye Vestine
School of Chemical and Biological Engineering, University of Lanzhou Jiaotong, Lanzhou 730000, China
Minggang Wang
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730000, China
Abdol Ghaffar Ebadi
Department of Agriculture, Jouybar Branch, Islamic Azad University, Jouybar, Iran
Mohsen Toughani
Department of Fishery, Islamic Azad University Babol Branch, Babol, Iran

Corresponding Author(s) : Mingjun Yang

ymingjun@bk.ru, yangmj@lut.cn

Cellular and Molecular Biology, Vol. 66 No. 2: Issue 2

Abstract

Codonopsis genus is comprised of species that are perennial plants primarily distributed across all east, southeast, and Central Asia. The most famous species of Codonopsis are C. tangshen, C. lanceolate, and C. pilosula. The records showed that they have a long story usage as traditional Chinese medicines, as they were alleged to be able to intensify the spleen and the lung as well as enriching blood and engendering liquid. Certain species have a culinary value in southern China and Southeast Asia, where they are considered as tea, wine, soup, plaster, and porridge. Codonopsis species were shown to be of great importance in medicine, due to their broad biological activity. Therefore, a clear understanding of their genetic diversity is needed.  Adequate distinctions and descriptions of those species are necessary to preserve plant reservoir, investigations of genes associated with desirable traits, and understanding of evolutionary relationships. Subsequently, various molecular marker techniques such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeats (SSR), and Inter Simple Sequence Repeat (ISSR), Single Nucleotide Polymorphism (SNP), internal transcribed spacer (ITS), and Sequence-Characterized Amplified Region (SCAR) have been improved to provide  detailed informations about genomes, that historically were  not possible to obtain based on only phenotypic methods. This review represents the usage of DNA molecular markers for molecular diversity analysis of medically important species belonging to the genus Codonopsis.

Keywords

Codonopsis species DNA Molecular markers Genetic diversity
Yang, M., Abdalrahman, H., Sonia, U., Mohammed, A. I., Vestine, U., Wang, M., Ebadi, A. G., & Toughani, M. (2020). The application of DNA molecular markers in the study of Codonopsis species genetic variation, a review. Cellular and Molecular Biology, 66(2), 23–30. https://doi.org/10.14715/cmb/2020.66.2.3
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References
  1. He JY, Ma N, Zhu S, Komatsu K, Li ZY, Fu WM. The genus Codonopsis (Campanulaceae): a review of phytochemistry, bioactivity and quality control. J Nat Med 2015; 69(1):1-21.
  2. Lin LC, Tsai TH, Kuo CL. Chemical constituents comparison of Codonopsis tangshen, Codonopsis pilosula var. modesta and Codonopsis pilosula. Nat Prod Res 2013; 27 (19):1812-5.
  3. Cosner ME, Raubeson LA, Jansen RK. Chloroplast DNA rearrangements in Campanulaceae: phylogenetic utility of highly rearranged genomes. BMC Evol Biol 2004; 4(1): 1-17.
  4. Wang Q, Ma XT, Hong DY. Phylogenetic analyses reveal three new genera of the Campanulaceae. J Syst Evol 2014; 52(5): 541-50. 5. Wang D, Cao LY, Gao JP. Data mining of simple sequence repeats in Codonopsis pilosula transcriptome. Chin. Tradit. Herbal Drugs. 2014; 45(16): 2390-4.
  5. Gao SM, Liu JS, Wang M, Cao TT, Qi YD, Zhang BG, Sun XB, Liu HT, Xiao PG. Traditional uses, phytochemistry, pharmacology and toxicology of Codonopsis: A review. J Ethnopharmacol 2018; 219: 50-70.
  6. He JY, Ma N, Zhu S, Komatsu K, Li ZY, Fu WM. The genus Codonopsis (Campanulaceae): a review of phytochemistry, bioactivity and quality control. J Nat Med 2015; 69(1):1-21.
  7. Dar AA, Dangroo NA, Raina A, Qayum A, Singh S, Kumar A, Sangwan PL. Biologically active xanthones from Codonopsis ovata.
  8. Phytochem 2016;132:102-8.
  9. Zhang P, Hu L, Bai R, Zheng X, Ma Y, Gao X, Sun B, Hu F. Structural characterization of a pectic polysaccharide from Codonopsis pilosula and its immunomodulatory activities in vivo and in vitro. Int. J. Biol. Macromol 2017;104:1359-69.
  10. Zhao Q, Wu YN, Fan Q, Han QQ, PareÌ PW, Xu R, Wang YQ, Wang SM, Zhang JL. Improved growth and metabolite accumulation in Codonopsis pilosula (Franch.) Nannf. by inoculation of Bacillus amyloliquefaciens GB03. J Agric Food Chem 2016; (64): 8103-8.
  11. He JY, Zhu S, Komatsu K, Goda Y, Cai SQ. Genetic polymorphism of medicinally-used Codonopsis species in an internal transcribed spacer sequence of nuclear ribosomal DNA and its application to authenticate Codonopsis Radix. J Nat Med 2014; 68(1):112-24. 12. Zheng YS, Wu ZS, Ni HB, Ke L, Tong ZH, Li WQ, Li N, Li JS. Codonopsis pilosula polysaccharide attenuates cecal ligation and puncture sepsis via circuiting regulatory T cells in mice. Shock 2014; 41(3): 250-5.
  12. Hu XY, Qin FY, Lu XF, Zhang LS, Cheng YX. Three new polyynes from Codonopsis pilosula and their activities on lipid metabolism. Molecules 2018; 23(4): 2-9.
  13. Fu YP, Feng B, Zhu ZK, Feng X, Chen SF, Li LX, Yin ZQ, Huang C, Chen XF, Zhang BZ, Jia RY. The polysaccharides from Codonopsis pilosula modulates the immunity and intestinal microbiota of cyclophosphamide-treated immunosuppressed mice. Molecules 2018; 23(7):1801.
  14. Shergis JL, Liu S, Chen X, Zhang AL, Guo X, Lu C, Xue CC. Dangshen [Codonopsis pilosula (Franch.) Nannf] herbal formulae for chronic obstructive pulmonary disease: A systematic review and meta-analysis. Phytother Res 2015; 29(2):167-86. 16. Ko PH, Huang CW, Chang HH, Chuang EY, Tsai MH, Lai LC. Identifying the functions and biomarkers of Codonopsis pilosula and Astragalus membranaceus aqueous extracts in hepatic cells.
  15. Chin Med 2019 Dec; 1-11.
  16. Yongxu S, Jicheng L. Structural characterization of a water-soluble polysaccharide from the roots of Codonopsis pilosula and its immunity activity. Int J Biol Macromol 2008; 43(3): 279-82. 18. Zhang Q, Xia Y, Luo H, Huang S, Wang Y, Shentu Y, Mahaman YA, Huang F, Ke D, Wang Q, Liu R. Codonopsis pilosula polysaccharide attenuates Tau hyperphosphorylation and cognitive impairments in hTau infected mice. Front Mol Neurosci 2018; 1-10.
  17. Yilmaz S, Marakli S, Yuzbasioglu G, Gozukirmizi N. Short-term mutagenicity test by using IRAP molecular marker in rice grown under herbicide treatment. Biotechnol Biotechnol Equip 2018; 32(4):
  18. -8.
  19. Yip PY, Chau CF, Mak CY, Kwan HS. DNA methods for identification of Chinese medicinal materials. Chin Med 2007 1;2(1): 1-19. 21. Biswas K, Biswas R. DNA molecular markers based authentication of herbal drugs-A review. Int J Pharm Res Scholars 2014; 3(1): 581-93.
  20. Hao D, Chen S, Xiao P, Peng Y. Authentication of medicinal plants by DNA-based markers and genomics. Chin Herb Med 2010; 2(4): 250-61.
  21. Karlik E, Tombuloğlu H. Molecular Markers and Their Applications. In. B. Plant Omics: Trends and Applications 2016;137-157.
  22. Springer, Cham.
  23. BUT PP, Pang-Chui SH. Identification of herbal medicinal materials using DNA barcodes. J Syst Evol 2011; 3:15.
  24. Joshi K, Chavan P, Warude D, Patwardhan B. Molecular markers in herbal drug technology. Curr Sci 2004; 25:159-65.
  25. Wang C, Guo Q, Wu Y. Genetic diversity of Changium smyrnioides based on SRAP. China
  26. J Chin Materia Med 2009; 34(24): 3180-3.
  27. Hayward AC, Tollenaere R, Dalton-Morgan J, Batley J. Molecular marker applications in plants. Plant Genotyping 2015;13-27. Humana Press, New York, NY.
  28. Nadeem MA, Nawaz MA, Shahid MQ, Doğan Y, Comertpay G, Yıldız M, Hatipoğlu R, Ahmad F, Alsaleh A, Labhane N, í–zkan H. DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnol.
  29. Biotechnol. Equip 2018; 32(2): 261-85.
  30. Al-Samarai FR, Al-Kazaz AA. Molecular markers: An introduction and applications. Eur J Mol Biotechnol 2015(3):118-30.
  31. Jiang G. Molecular Markers. 2017; 207-214.
  32. Lateef DD. DNA marker technologies in plants and applications for crop improvements. J Biosci Med 2015; 3(05): 7-18.
  33. Dhutmal RR, Mehtre SP, Mundhe AG. A Review on Molecular Approaches in Breeding for Abiotic Stress Tolerance. Int. J Curr Microbiol App Sci 2018; 816-825.
  34. McKnabb S, Rupp R, Tedesco JL. Measuring contaminating DNA in bioreactor derived monoclonals. Nat Biotechnol 1989; 7(4): 343-7.
  35. Sarwat M, Nabi G, Das S, Srivastava PS. Molecular markers in medicinal plant biotechnology: past and present. Crit Rev Biotechnol 2012; 32(1): 74-92.
  36. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Sci 1985; 230(4732):1350-4.
  37. Liu Y, Tan H, Qi Y, Ruan X, Liu N. DNA markers in the authentication of Traditional Chinese Medicine. J Med Plant Res 2014; 8(2):121-7.
  38. Um JY, Chung HS, Kim MS, Na HJ, Kwon HJ, Kim JJ, Lee KM, Lee SJ, Lim JP, Hwang WJ, Lyu YS. Molecular authentication of Panax ginseng species by RAPD analysis and PCR-RFLP. Biol Pharm Bull 2001; 24(8): 872-5.
  39. Guo HB, Lu BR, Wu QH, Chen JK, Zhou TS. Abundant genetic diversity in cultivated Codonopsis pilosula populations revealed by RAPD polymorphisms. Genet Resour Crop Ev 2007; 54(5): 917-24.
  40. Hu Y, Xie X, Wang L, Zhang H, Yang J, Li Y. Genetic variation in cultivated Rheum tanguticum populations. Genet Mol Biol 2014; 37(3): 540-8.
  41. Del Serrone P, Attorri L, Gallinella B, Gallo FR, Federici E, Palazzino G. Molecular identification of Panax ginseng CA Meyer in ginseng commercial products. Nat Prod Commun 2006;1(12):1137-
  43. Fu RZ, Wang J, Zhang YB, Wang ZT, But PP, Li N, Shaw PC. Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism. Planta Med 1999 ;65(07): 648-50.
  44. Kaundun SS, Matsumoto S. Identification of Processed Japanese Green Tea Based on Polymorphisms Generated by STS-RFLP Analysis. J Agric Food Chem 2003; 51(7): 1765-70.
  45. Mizukami H, Okabe Y, Kohda H, HIRAOKA N. Identification of the crude drug atractylodes rhizome (Byaku-jutsu) and atractylodes lancea rhizome (So-jutsu) using chloroplast TrnK sequence as a molecular marker. Biol Pharm Bull 2000; 23(5): 589-94. 44. Calonje M, Martí­n-Bravo S, Dobeš C, Gong W, Jordon-Thaden I, Kiefer C, Kiefer M, Paule J, Schmickl R, Koch MA. Non-coding nuclear DNA markers in phylogenetic reconstruction. Plant Syst Evol 2009; 282(3-4): 257-80.
  46. Group CP, Li DZ, Gao LM, Li HT, Wang H, Ge XJ, Liu JQ, Chen ZD, Zhou SL, Chen SL, Yang JB. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. Proc. Natl. Acad. Sci 2011;108(49):19641-6.
  47. Lin T, Hsieh C, Agrawal DC, Kuo C, Chueh F, Tsay H. ITS sequence based phylogenetic relationship of dangshen radix. J Food Drug Anal 2007;15(4): 428-432.
  48. Navajas M, Fenton B. The application of molecular markers in the study of diversity in acarology: a review. Exp Appl Acarol 2000; 24(10-11): 751-74.
  49. López-Flores I, Garrido-Ramos MA. The repetitive DNA content of eukaryotic genomes. Genome Dyn 2012; 7, 1-28. Karger Publishers.
  50. Li ZH, Wen HY, Chen J, Wu GL, Wang YJ. Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf.(Campanulaceae). Conserv Genet 2009;10(3):747-9.
  51. Kim S, Jeong JH, Chung H, Kim JH, Gil J, Yoo J, Um Y, Kim OT, Kim TD, Kim YY, Lee DH. Simple sequence repeat marker development from Codonopsis lanceolata and genetic relation analysis. J Plant Biotechnol 2016; 43(2):181-8.
  52. Tautz D. Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Res 1989; 17(16): 6463-71.
  53. HB G. The Genetic Diversity of Wild and Cultivated Populations of Codonopsis pilosula and Implications for Conservation (Doctoral dissertation, PhD Thesis. Shanghai: Fudan University) 2007. 53. Barth S, Melchinger AE, Lübberstedt TH. Genetic diversity in
  54. Arabidopsis thaliana L. Heynh. investigated by cleaved amplified polymorphic sequence (CAPS) and inter-simple sequence repeat (ISSR) markers. Mol Ecol 2002; 11(3): 495-505.
  55. Nagaoka T, Ogihara Y. Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 1997; 94(5):
  56. -602.
  57. Wang XM. Inter-simple sequence repeats (ISSR) molecular fingerprinting markers for authenticating the genuine species of rhubarb. J Med Plant Res 2011; 5(5): 758-64.
  58. Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990;18(22): 6531-5.
  59. Pourmohammad A. Application of molecular markers in medicinal plant studies. Acta Univ. Sapientiae, Agric Environ 2013; 5(1):80-90.
  60. Sucher NJ, Carles MC. Genome-based approaches to the authentication of medicinal plants. Planta Med 2008;74(06):603-23.
  61. Joshi K, Chavan P, Warude D, Patwardhan B. Molecular markers in herbal drug technology. Curr Sci 2004;159-65.
  62. Yu G, MA YX, Chen JW. Analysis of Genetic Variation in Radix Changii During Planting by RAPD Fingerprinting Method. J Chengdu Univ Tradit Chin Med 2009; (2):32.
  63. Guo WL, Gong L, Ding ZF, Li YD, Li FX, Zhao SP, Liu B. Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook. f., as revealed by ISSR and RAPD markers. Plant Cell Rep 2006; 25(9): 896-906.
  64. Devaiah K, Balasubramani SP, Venkatasubramanian P. Development of randomly amplified polymorphic DNA based SCAR marker for identification of Ipomoea mauritiana Jacq (Convolvulaceae).
  65. Evid Based Complemen Altern Med 2011; (1): 1-6.
  66. Balasubramani SP, Goraya GS, Venkatasubramanian P. Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb. 3 Biotech 2011;1(1):11-19.
  67. Balasubramani SP, Murugan R, Ravikumar K, Venkatasubramanian P. Development of ITS sequence based molecular marker to distinguish, Tribulus terrestris L. (Zygophyllaceae) from its adulterants. Fitoterapia 2010; 81(6):503-8.
  68. Moon BC, Kim WJ, Han KS, Yang S, Kang Y, Park I, Piao R. Differentiating authentic Adenophorae Radix from its adulterants in commercially-processed samples using multiplexed ITS sequencebased SCAR markers. Appl Sci 2017; 7(7):660. 66. Wang DY, Wang Q, Wang YL, Xiang XG, Huang LQ, Jin XH. Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera. PloS one 2017; 1-14. 67. Vos P, Hogers R, Bleeker M, Reijans M, Vande LT, Hornes M, Fritjers A, Pot J, Peleman J, Kuiper M, Zabeau M. A new concept for DNA fingerprinting. Nucl Acids Res 1995; 23:4407-14.
  69. Chester K, Tamboli ET, Paliwal SK, Ahmad S. Significance of molecular markers in pharmacognosy: A modern tool for authentication of herbal drugs. Drug Des. Dev. Ther 2016; 7(2): 96-106. 69. Gu C, Cao LY, Su Q, Guan LJ, Yang J, Gao JP. AFLP and HPLC Fingerprints Analysis of Codonopsis Species from Original Areas and the Same Planting Base. J. Chin. Med. Mater 2016; 39(8):171622.
  70. Marakli S. A Brief Review of Molecular Markers to Analyse Medically Important Plants. Int J. Life Sci. Biotechnol 2018; 29-36.
  71. Wang CM, Liu P, Yi C, Gu K, Sun F, Li L, Lo LC, Liu X, Feng F, Lin G, Cao S. A first generation microsatellite-and SNP-based linkage map of Jatropha. PloS one 2011; 4-11.
  72. Mammadov J, Aggarwal R, Buyyarapu R, Kumpatla S. SNP markers and their impact on plant breeding. Int. J. Plant Genomics 2012; 1-11.
  73. Hwang SG, Kim JH, Moon JC, Kim JH, Jang CS. Comparative analysis of chloroplast DNA sequences of Codonopsis lanceolata and Platycodon grandiflorus and application in development of molecular markers. Appl. Biol. Chem 2017; 60(1):23-31. 74. Jo IH, Sung J, Hong CE, Raveendar S, Bang KH, Chung JW. Development of cleaved amplified polymorphic sequence (CAPS) and high-resolution melting (HRM) markers from the chloroplast genome of Glycyrrhiza species. 3 Biotech 2018; 8(5):220.
  74. Shekhawat JK, Rai MK, Shekhawat NS, Kataria V. Start codon targeted (SCoT) polymorphism for evaluation of genetic diversity of wild population of Maytenus emarginata. Ind. Crops Prod 2018;122:202-8.
  75. Zhang J, Xie W, Wang Y, Zhao X. Potential of start codon targeted (SCoT) markers to estimate genetic diversity and relationships among Chinese Elymus sibiricus accessions. Molecules 2015; 20(4):5987-6001.
  76. Tiwari G, Singh R, Singh N, Choudhury DR, Paliwal R, Kumar A, Gupta V. Study of arbitrarily amplified (RAPD and ISSR) and gene targeted (SCoT and CBDP) markers for genetic diversity and population structure in Kalmegh [Andrographis paniculata (Burm. f.) Nees]. Ind. Crops Prod 2016; 86:1-1.
  77. Luscombe NM, Greenbaum D, Gerstein M. What is bioinformatics? A proposed definition and overview of the field. Methods of information in medicine. 2001;40(04): 346-58.
  78. Harishchander A. A review on application of bioinformatics in medicinal plant research. Bioinf. Proteomics. Open. Access. J 2017;1(1):000104.
  79. Liang Y, Lin Q, Huang P, Wang Y, Li J, Zhang L and Cao J: Rice Bioactive Peptide Binding with TLR4 To Overcome H2O2-Induced Injury in Human Umbilical Vein Endothelial Cells through NF-κB Signaling. J Agri Food Chem 2018; 66(2): 440-448.
  80. Wang L, Lin Q, Yang T, Liang Y, Nie Y, Luo Y and Luo F: Oryzanol modifies high fat diet-induced obesity, liver gene expression profile, and inflammation response in mice. J Agri Food Chem 2017; 65(38): 8374-8385.
  81. Lou Y, Shi J, Guo D, Qureshi AK and Song L: Function of PDL1 in antitumor immunity of glioma cells. Saudi J Biol Sci 2017:
  82. (4): 803-807.
  83. Guo T, Lin Q, Li X, Nie Y, Wang L, Shi L and Luo F. Octacosanol attenuates inflammation in both RAW264. 7 macrophages and a mouse model of colitis. J Agri Food Chem 2017: 65(18): 3647-3658. 84. Li W, Jia MX, Wang JH, Lu JL, Deng J, Tang JX and Liu C: Association of MMP9-1562C/T and MMP13-77A/G polymorphisms with non-small cell lung cancer in southern Chinese population. Biomol 2019; 9(3): 107-119.
  84. Nie Y, Luo F, Wang L, Yang T, Shi L, Li X, Shen J, Xu W, Guo T and Lin Q: Anti-hyperlipidemic effect of rice bran polysaccharide and its potential mechanism in high-fat diet mice. Food Func 2017; 8(11): 4028-4041.
  85. Lou Y, Yang J, Wang L, Chen X, Xin X and Liu Y: The clinical efficacy study of treatment to Chiari malformation type I with syringomyelia under the minimally invasive surgery of resection of Submeningeal cerebellar Tonsillar Herniation and reconstruction of Cisterna magna. Saudi J Biol Sci 2019: 26(8): 1927-1931.
  86. Lou Y, Guo D, Zhang H and Song L: Effectiveness of mesenchymal stems cells cultured by hanging drop vs. conventional culturing on the repair of hypoxic-ischemic-damaged mouse brains, measured by stemness gene expression. Open Life Sci 2016: 11(1): 519-523. 88. Chen X, Xu Y, Meng L, Chen X, Yuan L, Cai Q, Shi W and Huang G: Non-parametric partial least squares–discriminant analysis model based on sum of ranking difference algorithm for tea grade identification using electronic tongue data identify tea grade using e-tongue data. Sens Actuators B Chem 2020; 127924. 89. Nie Y, Luo F and Lin Q: Dietary nutrition and gut microflora: A promising target for treating diseases. Trends Food Sci Technol 2018; 75: 72-80.
  87. Ren Y, Jiao X and Zhang L: Expression level of fibroblast growth factor 5 (FGF5) in the peripheral blood of primary hypertension and its clinical significance. Saudi J Biol Sci 2018; 25(3): 469-473.
  88. Raja HA, Miller AN, Pearce CJ, Oberlies NH. Fungal identification using molecular tools: a primer for the natural products research community. J. Nat. Prod 2017;80(3):756-70.
  89. Gao JP, Wang D, Cao LY, Sun HF. Transcriptome sequencing of Codonopsis pilosula and identification of candidate genes involved in polysaccharide biosynthesis. PloS one 2015; 1-25. 93. Babar MM, Pothineni VR, Ali Z, Faisal S, Hakeem KR, Gul A. Application of bioinformatics and system biology in medicinal plant studies. Plant. Bioinf 2017; 375-393. Springer, Cham.
  90. Gupta S, Bharalee R, Das R, Thakur D. Bioinformatics tools for development of fast and cost effective simple sequence repeat (SSR), and single nucleotide polymorphisms (SNP) markers from expressed sequence tags (ESTs). Afr. J. Biotechnol 2013; 4713-4721.
  91. Cao LY, Li XX, Wang D, Sun HF, Gao JP. Validation of reliable reference genes for accurate normalization in RT-qPCR analysis of Codonopsis pilosula. Chin. Herb. Med 2017; 9(3):226-35.
  92. Rostami-Ahmadvandi H, Cheghamirza K, Kahrizi D, Bahraminejad S. Comparison of morpho-agronomic traits versus RAPD and ISSR markers in order to evaluate genetic diversity among Cuminum cyminum L. accessions. Aust J Crop Sci 2013; 7(3):361-367. 97. Ghafari Azar A, Darvishzadeh R, Aghaali Z, Kahrizi D, Darvishi B. Assessment of genetic diversity and grouping of maize lines (Zea mays L.) using ISSR markers. J Cell Mol Res 2019; 32(2): 194-204. 98. Safari H, Zebarjadi A, Kahrizi D, Jafari AA. The study of interspecific relationships of Bromus genus based on SCoT and ISSR molecular markers. Mol Biol Rep 2019; 46(5): 5209–5223. 99. Asadi N, Rahimi A, Ghaheri M, Kahrizi D, Bagheri Dehbaghi M, Khederzadeh S, Esmaeilkhanian S, Veisi B, Geravandi M, Karim H, Vaziri S, Daneshgar F, Banabazi MH, Zargooshi J. Genetic diversity of the Dwarf honeybee (Apis florea Fabricius, 1787) populations based on microsatellite markers. Cell Mol Biol 2016; 62(2): 51-55. 100. Rahimi A, Mirmoayedi A, Kahrizi D, Zarei L, Jamali S. Genetic diversity of Iranian honey bee (Apis mellifera meda Skorikow, 1829) populations based on ISSR markers. Cell Mol Biol 2016; 62(4): 55-60.
  93. Yari K, Mirmoayedi A, Marami M, Kazemi E, Kahrizi D. Genetic diversity analysis of chrysopidae family (insecta, neuroptera) via molecular markers. Molecular Biology Reports. 2013; 41:62416245.
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