MicroRNA214 expression inhibits HCC cell proliferation through PTK2b/ Pyk2

Cui  Chen; Zhen  Tao; Ya  Li; Jie  Li; Yang  Xu

doi:10.14715/cmb/2022.68.1.4

Issue

Vol. 68 No. 1: Issue 1

Issue Published : May 22, 2022

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MicroRNA214 expression inhibits HCC cell proliferation through PTK2b/ Pyk2

https://doi.org/10.14715/cmb/2022.68.1.4

Cui Chen

Department of Hepatobiliary Surgery, The First College of Clinical Medical Science, Three Gorges University（Yichang Central People’s Hospital), Yichang, Hubei443003, China

Zhen Tao

Department of Emergency, Huangshi Central Hospital（Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei435000, China

Ya Li

Department of Internal Medicine, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi710061, China

Jie Li

Department of Hepatobiliary Surgery, Fuyang People's Hospital in Anhui, Fuyang, Anhui236000, China

Yang Xu

Department of Integrated Traditional Chinese and Western Medicine, Wuhan Third Hospital, Wuhan, Hubei430060, China

Corresponding Author(s) : Yang Xu

xuyangsci2021@163.com

Cellular and Molecular Biology, Vol. 68 No. 1: Issue 1
Article Published : May 22, 2022

Abstract

MicroRNAs (miRNAs/miRs) are crucial regulatory molecules that act as the most significantly downregulated microRNAs in hepatocellular carcinoma (HCC). PTK2b/Pyk2 is a non-receptor protein tyrosine kinase, which plays an important role in the development and metastasis of cancer. In this study, we explored the expression level and functional relationship between MicroRNA-214 (miR-214) and PTK2b/ Pyk2 in liver cancer cells. For this purpose, we analyzed the expression of miR-214 and PTK2b/Pyk2 in 38 cases of HCC and paired non-neoplastic tissue specimens using real-time PCR. MTT, cell cycle and construct recombinant plasmids analysis were used to explore the effects of miR-214 and PTK2b/Pyk2 on liver cancer cell proliferation. Results showed that the expression level of mir-214 in liver cancer tissues and liver cancer cell lines was significantly lower than that in normal tissues and cells, while the expression of PTK2b/Pyk2 was significantly increased. The overexpression of mir-214 or inhibition PTK2b/Pyk2 inhibited the proliferation of HCC cells. This research showed that mir-214 has an inhibitory effect on liver cancer through the expression of PTK2b/Pyk2.

Chen, C. ., Tao, Z. ., Li, Y. ., Li, J. ., & Xu, Y. . (2022). MicroRNA214 expression inhibits HCC cell proliferation through PTK2b/ Pyk2. Cellular and Molecular Biology, 68(1), 20–25. https://doi.org/10.14715/cmb/2022.68.1.4