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Copyright (c) 2023 Awad Algani
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Evaluation of plastid and nuclear DNA markers in barcoding of Aloe saudiarabica, KSA
Corresponding Author(s) : Awad Algarni
Cellular and Molecular Biology,
Vol. 69 No. 2: Issue 2
Abstract
There is great plant diversity in Saudi Arabia. The Asphodelaceae family is within this great diversity, especially the rare species such as the plant, Aloe saudiarabica. Such plants must be preserved in their natural ranges, hence, the need to document them. Genetic markers have become the approved and widely used method for documenting rare plants. The current study deals with the use of three genetic markers to document A. saudiarabica for the first time. The used genetic markers were Maturase-K (matK), Ribulose-bisphosphate-carboxylase (rbcL), and Internal-transcribed-spacer (ITS). The study found that the primers used for the rbcL gene were not effective in achieving identification. Sequencing of the matK and ITS were achieved successfully. The sequences were determined for both markers using two pairs of primers and deposited in the NCBI databases (GenBank). These markers were effective in identifying A. saudiarabica and determining its evolutionary relationship with other Aloe species in various databases. The study showed that A. vera is high similar (>99%) to the other species. In conclusion, the study showed the likelihood of the different genetic markers to document A. saudiarabica, especially the currently investigated matK and ITS.
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