Haseeb A. Khan; N. Rajendra Prasad; Salman H. Alrokayan; Amani A. Alghamdi

doi:10.14715/cmb/2023.69.10.19

Issue

Vol. 69 No. 10: Issue 10

Issue Published : October 31, 2023

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Antiproliferative effect of Solanum nigrum L. water extract on breast can-cer cells: potential roles of apoptosis and oxidative stress

Antiproliferative effect of Solanum nigrum on breast cancer cells

https://doi.org/10.14715/cmb/2023.69.10.19

Haseeb A. Khan

Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

https://orcid.org/0000-0001-6084-8589

N. Rajendra Prasad

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, India

Salman H. Alrokayan

Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Amani A. Alghamdi

Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Corresponding Author(s) : Haseeb A. Khan

khan_haseeb@yahoo.com

Cellular and Molecular Biology, Vol. 69 No. 10: Issue 10
Article Published : October 31, 2023

Abstract

Breast cancer is the most progressive cancer among women worldwide. The currently available chemotherapeutic agents induce severe unacceptable adverse effects in breast cancer patients. In this context, natural medicinal herbs are gaining importance to find non-toxic effective anticancer drugs. Solanum nigrum is one of the major traditional medicinal plants widely used in Ayurveda for the treatment of various diseases. This study investigated the anticancer effect of Solanum nigrum water extract (SNWE) against MCF-7 and triple-negative MDA-MB-231 breast cancer cell lines. SNWE significantly induced oxidative stress-mediated apoptotic cell death in a concentration-dependent manner. Real-time PCR results illustrated the upregulation of proapoptotic genes and downregulation of antiapoptotic genes after SNWE treatment in MCF-7 and MDA-MB-231 cell lines. Immunofluorescence analysis showed increased expressions of apoptotic markers like p53, Caspase3 and BAX by SNWE treatment. In conclusion, the findings of this study indicate the antiproliferative effect and apoptosis-inducing property of SNWE in both cell lines. Further studies are warranted on testing the anticancer activity of S. nigrum L. using animal models of cancer.

Keywords

Solanum nigrum Anticancer activity Breast cancer Cytotoxicity Apoptosis Oxidative stress

Khan, H. A., Prasad, N. R., Alrokayan, S. H., & Alghamdi, A. A. (2023). Antiproliferative effect of Solanum nigrum L. water extract on breast can-cer cells: potential roles of apoptosis and oxidative stress: Antiproliferative effect of Solanum nigrum on breast cancer cells. Cellular and Molecular Biology, 69(10), 136–142. https://doi.org/10.14715/cmb/2023.69.10.19