Jun Huang; Wentao Jiang; Junping Pan; Xuetao Xu

doi:10.14715/cmb/2023.69.10.21

Issue

Vol. 69 No. 10: Issue 10

Issue Published : October 31, 2023

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Correlation of sLOX-1 and CSF1 with the pathological progression of hypoxic-ischemic encephalopathy in neonatal rats

Relationship between sLOX-1, CSF1 and hypoxic-ischemic brain

https://doi.org/10.14715/cmb/2023.69.10.21

Jun Huang

Department of Pediatrics, Chaohu Hospital Affiliated to Anhui Medical University, Hefei, Anhui,238001, China

Wentao Jiang

Functional Laboratory, Anhui Medical College, Hefei, Anhui,238001, China

Junping Pan

Department of Pediatrics, Hefei First People's Hospital, the Third Affiliated Hospital of Anhui Medical University, Hefei, Anhui,238001, China

Xuetao Xu

Department of Pediatrics, Chaohu Hospital Affiliated to Anhui Medical University, Hefei, Anhui,238001, China

Corresponding Author(s) : Jun Huang

hj1460780930@163.com

Cellular and Molecular Biology, Vol. 69 No. 10: Issue 10
Article Published : October 31, 2023

Abstract

To provide clinical evidence for the management of hypoxic-ischemic encephalopathy (HIE) by analyzing the role of soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) and colony-stimulating factor-1 (CSF1) in the disease. We purchased 15 Sprague-Dawley (SD) rat pups and randomized them into five groups (n=3), of which one group was untreated as the control group and the other four were modeled by HIE. After modeling, a group was treated as a model group without any treatment, another group was injected with sLOX-1-silencing lentiviral vector (sLOX-1-si group), and the third and fourth were injected with CSF1-silencing lentiviral vector (CSF1-si group) and an equal amount of normal saline (blank group), respectively. After the corresponding intervention, the rat tissue in each group was obtained to observe the pathological injury by HE and TUNEL staining. In addition, sLOX-1, CSF1, 5-hydroxytryptamine (5-HT), dopamine (DA), and norepinephrine (NE) levels in brain tissue of each group were determined. The model group showed more severe pathological damage of the hippocampus and higher neuronal apoptosis than the control group. Besides, higher sLOX-1 and CSF1 levels and lower 5-HT, DA and NE contents were identified in the model group versus the control group (P<0.05). Compared with the blank group, sLOX-1-si and CSF1-si groups showed significantly alleviated hippocampal damage, inhibited neuronal apoptosis, reduced 5-HT, DA, NE, Bax, and cl-caspase-3, and increased Bcl-2 (P<0.05). Silencing sLOX-1 and CSF1 expression ameliorated the pathological injury of HIE and inhibited neuronal apoptosis.

Keywords

hypoxic-ischemic encephalopathy low-density lipoprotein receptor-1 colony stimulating factor-1 apoptosis of neuronal cells seahorse district injuries

Huang, J., Jiang, W., Pan, J., & Xu, X. (2023). Correlation of sLOX-1 and CSF1 with the pathological progression of hypoxic-ischemic encephalopathy in neonatal rats: Relationship between sLOX-1, CSF1 and hypoxic-ischemic brain. Cellular and Molecular Biology, 69(10), 150–154. https://doi.org/10.14715/cmb/2023.69.10.21