ABM Sharif Hossain; Hassan A. Alrudayni; Faouzi Haouala; Mohammed Saad Aleissa; Danial Kahrizi; Abu Saleh Ahmed

doi:10.14715/cmb/2023.69.11.8

Issue

Vol. 69 No. 11: Issue 11

Issue Published : November 28, 2023

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Callus cell and explants regeneration, glucose, mineral, antioxidant and flavonoid content development using broccoli root tip and leaf cutting in vitro

In vitro broccoli root tip and leaf cutting culture

https://doi.org/10.14715/cmb/2023.69.11.8

ABM Sharif Hossain

Department of Biology, College of Science, Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi Arabia

Hassan A. Alrudayni

Department of Biology, College of Science, Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi Arabia

Faouzi Haouala

Department of Biology, College of Science, Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi Arabia

Mohammed Saad Aleissa

Department of Biology, College of Science, Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi Arabia

Danial Kahrizi

Agricultural Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

Abu Saleh Ahmed

School of Engineering and Technology, University of Technology Sarawak, Malaysia

Corresponding Author(s) : ABM Sharif Hossain

hossainsharif41@gmail.com

Cellular and Molecular Biology, Vol. 69 No. 11: Issue 11
Article Published : November 15, 2023

Abstract

The study was conducted to evaluate the root, shoot and leaf callus cell regeneration and its biochemical properties like antioxidant, carbohydrate, pigment and mineral content from broccoli root, shoot and leaf cutting in vitro. An in vitro factorial experiment was carried out based on a Completely Randomized Design (CRD) with 5 replicates in tissue culture applying different IBA (0.25, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 mg/l) and BAP (1 mg/l) concentrations using broccoli root tip and leaf cutting. The results showed that a higher callus weight was found in the cultured leaf cutting than in root tip cutting in the concentration of 1.0, 1.5 & 2.0 mg/l IBA + 1.0 mg/l BAP combination. The highest callus weight was found in the cultured leaf cutting than root tips cutting at the concentration of 1.5mg/l IBA+1.0 mg/l BAP. Furthermore, the highest inverted sugar and glucose, chlorophyll and nutrient content (K+, NO₃- & Ca++), total phenol, flavonoid and total antioxidant were found in the concentration of 1.5mg/l IBA+1.0 mg/l BAP combination in both broccoli leaf and root cutting. The results seemed that it was best to use the combination of the IBA and BAP in the concentration of 1.0-2.0 mg/l and 1mg/l to regenerate root, leaf and callus cell proliferation of broccoli from the root tip and leaf cutting.

Keywords

Root, leaf, Callus Cell, Broccoli, Antioxidant

Hossain, A. S., Alrudayni, H. A., Haouala, F., Aleissa, M. S., Kahrizi, D., & Ahmed, A. S. (2023). Callus cell and explants regeneration, glucose, mineral, antioxidant and flavonoid content development using broccoli root tip and leaf cutting in vitro: In vitro broccoli root tip and leaf cutting culture. Cellular and Molecular Biology, 69(11), 45–50. https://doi.org/10.14715/cmb/2023.69.11.8