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Copyright (c) 2023 Filiz Tepekoy, Gokhan Akkoyunlu
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.The effects of MEK and PKA inhibition on Spire proteins during oocyte maturation
Corresponding Author(s) : Gokhan Akkoyunlu
Cellular and Molecular Biology,
Vol. 69 No. 13: Issue 13
Abstract
Asymmetric division of oocytes driven by chromosome migration is a crucial step of oocyte maturation. Actin filaments take key roles in chromosome migration in oocytesThe aim of this study was to determine the effects of MEK and PKA inhibition on the levels of Spire-1 and Spire-2 proteins that are known to be related to actin nucleation.MEK inhibitor PD98059 and PKA inhibitor H89 were applied during IVM to the oocytes retrieved from preovulatory ovarian follicles of PMSG induced 3-5 weeks old female BalbC mice. GVBD and PBE rates were determined. Spire-1 and Spire-2 proteins were detected by immunofluorescence and western blot in oocytes at different maturation stages.Though GVBD rates were similar in different groups, PBE rates were lower in the MEK inhibition group. Through immunofluorescence, cortical localizations of Spire-1 and Spire-2 were determined. MEK inhibition resulted in a decrease in cortical Spire-1 and Spire-2 levels in PBE oocytes. PKA inhibition led to an increase in cortical Spire-1 levels in spindle migration stage oocytes, and an increase in cortical and total Spire-2 levels in PBE oocytes. Application of both MEK and PKA inhibition resulted in compensation of the decrease in Spire-1, while Spire-2 levels remained low with no compensation of PKA inhibition.According to the results of this study, chemical inhibition of MEK and PKA during oocyte maturation alters Spire-1 and Spire-2 protein levels.
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