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Copyright (c) 2025 wei liu, Yunfeng NIU, Jie An
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Long non-coding RNA LINC00520 promotes malignant progression of gastric adenocarcinoma through miR-519b-3p/HIF1A axis
Corresponding Author(s) : Wei Liu
Cellular and Molecular Biology,
Vol. 71 No. 2: Issue 2
Abstract
Gastric cancer is a prevalent malignant tumor, characterized by high morbidity and mortality rates globally. Long non-coding RNAs (lncRNAs), a class of transcripts exceeding 200 nucleotides in length, are non-protein-coding molecules that exert crucial regulatory functions in cellular biology. Investigating the regulatory mechanisms of lncRNAs in gastric cancer is essential. This study aimed to elucidate the functional role and molecular mechanisms of LINC00520 in gastric cancer. Initially, the GEO database was screened for differentially expressed genes associated with the malignant progression of gastric cancer. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was utilized to ascertain the LINC00520 expression in gastric cancer tissues. Subsequently, cellular functional assays were conducted to investigate the potential effects of LINC00520 on cellular behavior. The interaction between LINC00520, miR-519b-3p, and HIF1A was examined through bioinformatics analysis, and their binding interactions were confirmed using dual-luciferase reporter gene assays and RNA immunoprecipitation (RIP) assays. Our findings revealed a marked increase in the LINC00520 expression in gastric cancer tissues. Overexpression of LINC00520 was observed to enhance the malignant progression of gastric cancer cells. Through bioinformatics analysis, dual-luciferase reporter assays, and RIP assays, we demonstrated that LINC00520 upregulated HIF1A expression by competitively binding to miR-519b-3p, thereby acting as a molecular sponge. In conclusion, this study indicates that LINC00520, which is highly expressed in gastric cancer, exerts its effects by targeting the miR-519b-3p/HIF1A axis. These insights provide a foundation for developing diagnostic and therapeutic strategies for gastric cancer.
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