Issue
Copyright (c) 2025 Moayad Baazeem, Abdurahman Niazy, Sameh Rabea, Abdulaziz Alomiery
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Inhibitory potential of probiotic strains against pathogens associated with osteomyelitis of the jaws: a quantitative MIC and MBC analysis
Corresponding Author(s) : Moayad Baazeem
Cellular and Molecular Biology,
Vol. 71 No. 6: Issue 6
Abstract
Osteomyelitis of the jaw is a significant health burden, often associated with biofilm formation, antimicrobial resistance, and complex bacterial interactions. This study aimed to evaluate the antibacterial efficacy of two probiotic strains, Lactobacillus reuteri DSM 17938 and ATCC PTA 6475, against three key pathogens: Fusobacterium nucleatum, Actinomyces israelii, and Staphylococcus aureus. The focus was on their potential as adjunctive treatments for osteomyelitis of the jaws. This in-vitro study employed tailored standard protocols to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the probiotic strains against the tested pathogens. Probiotic suspensions were prepared in serial dilutions and bacterial strains were inoculated into brain heart infusion media for exposure. The growth inhibition and eradication were confirmed through subculture techniques and microscopic examination of Gram-stained samples. Both probiotic strains exhibited significant antibacterial activity against Fusobacterium nucleatum and Actinomyces israelii at low concentrations, achieving MIC of 0.625 µl and MBC of 1.25 µl for both pathogens. In contrast, Staphylococcus aureus demonstrated greater resistance, with MIC and MBC recorded at 100 µl and 200 µl, respectively. The differential response underscores the variable susceptibility of bacterial species to probiotics and highlights the potential for selective therapeutic application. Within the scope of this study, two Lactobacillus reuteri strains demonstrated promising antibacterial effects against Fusobacterium nucleatum and Actinomyces israelii, suggesting their potential utility in managing osteomyelitis of the jaws. However, higher concentrations or synergistic approaches may be needed to combat Staphylococcus aureus. Further studies including preclinical and clinical trials are essential to translate these findings into effective therapeutic strategies.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
