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Copyright (c) 2025 Abdul Nasser H. Warwar, Mohammed I. Abdullah, Wesam A. Sami, Waleed Mohammed
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Histological changes in dental pulp tissue with age: a comparative study
Corresponding Author(s) : Abdul Nasser H. Warwar
Cellular and Molecular Biology,
Vol. 71 No. 6: Issue 6
Abstract
The dental pulp undergoes several histological changes with age. These include a reduction in the number of cells and blood vessels, resulting in a decreased capacity for regeneration and repair. Furthermore, there is an increase in collagen fiber density and the formation of secondary dentin, which reduces the volume of the pulp chamber. This study performed a comparative histological analysis to characterize these age-related changes in dental pulp tissues. A cross-sectional comparative analysis was conducted to examine the histological changes in dental pulp tissue from two distinct age groups. Extracted teeth were thoroughly cleaned and immediately fixed in 10% formalin for preservation. Each sample was then decalcified using 10% formic acid for a duration sufficient to allow sectioning without damaging the tissue. After decalcification, the teeth were embedded in paraffin wax, and serial sections, with a thickness of 4-6 μm, were prepared using a microtome. The sections were then stained with Hematoxylin and Eosin (H&E) to visualize general tissue structure. The histological analysis demonstrated notable differences in dental pulp tissue between the two age groups. Group A (young) samples showed a high cellularity, with numerous fibroblasts and odontoblasts and a clearly defined odontoblastic layer. However, Group B (elderly) samples exhibited a marked reduction in cellularity, with fewer odontoblasts present and evidence of increased fibroblast degeneration. The histological changes observed in our study underscore the impact of aging on dental pulp tissue.
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